Results 21 to 30 of about 283,742 (346)

Fluorescence In Situ Hybridization [PDF]

Journal of Investigative Dermatology, 2013
Fluorescence in situ hybridization (FISH) is a cytogenetic technique used to detect the presence or absence and location of specific gene sequences. It can visualize specific cytogenetic abnormalities (copy number aberrations) such as chromosomal deletion, amplification, and translocation. FISH has been used in prenatal diagnosis and has served both as
Chen, Amy Y.-Y., Chen, Andrew
openaire   +2 more sources

Correlation of HER2, MDM2, c-MYC, c-MET, and TP53 Copy Number Alterations in Circulating Tumor Cells with Tissue in Gastric Cancer Patients: A Pilot Study [PDF]

Iranian Biomedical Journal, 2020
Background: The analysis of the gene copy number alterations in tumor samples are increasingly used for diagnostic and prognostic purposes in patients with GC. However, these procedures are not always applicable due to their invasive nature.
Fatemeh Nevisi, Marjan Yaghmaie, Hossein Pashaiefar, Kamran Alimoghaddam, Masoud Iravani, Gholamreza Javadi, Ardeshir Ghavamzadeh   +6 more
doaj   +1 more source

Karyotype Differentiation in Cultivated Chickpea Revealed by Oligopainting Fluorescence in situ Hybridization

Frontiers in Plant Science, 2022
Chickpea (Cicer arietinum L.) is one of the main sources of plant proteins in the Indian subcontinent and West Asia, where two different morphotypes, desi and kabuli, are grown.
Alžběta Doležalová, Lucia Sládeková, D. Šimoníková, K. Holušová, M. Karafiátová, R. Varshney, J. Doležel, E. Hřibová   +7 more
semanticscholar   +1 more source

Detection of fluorescence in situ hybridization on human metaphase chromosomes by near-field scanning optical microscopy [PDF]

, 1995
Fluorescence in situ hybridization signals o­n human metaphase chromosomes are detected by a near-field scanning optical microscope. This makes it possible to localize and identify several fluorescently labeled genomic DNA fragments o­n a single ...
Jalocha, A., Jalocha, A., Moers, M.H.P., Moers, M.H.P., Ruiter, A.G.T., van Hulst, N.F.   +5 more
core   +6 more sources

Multiple fluorescence in situ hybridization [PDF]

Cytometry, 1990
AbstractA method for multiple fluorescence in situ hybridization is described allowing the simultaneous detection of more than three target sequences with only three fluorescent dyes (FITC, TRITC, AMCA), respectively emitting in the green, red, and blue.This procedure is based on the labeling of (DNA) probes with more than one hapten and visualisation ...
P M, Nederlof, S, van der Flier, J, Wiegant, A K, Raap, H J, Tanke, J S, Ploem, M, van der Ploeg   +6 more
openaire   +2 more sources

In situ visualization of bacterial populations in coral tissues: pitfalls and solutions [PDF]

PeerJ, 2016
In situ visualization of microbial communities within their natural habitats provides a powerful approach to explore complex interactions between microorganisms and their macroscopic hosts.
Naohisa Wada, Frederic J. Pollock, Bette L. Willis, Tracy Ainsworth, Nobuhiro Mano, David G. Bourne   +5 more
doaj   +2 more sources

Use of Fluorescence In Situ Hybridization (FISH) in Diagnosis and Tailored Therapies in Solid Tumors

Molecules, 2020
Fluorescence in situ hybridization (FISH) is a standard technique used in routine diagnostics of genetic aberrations. Thanks to simple FISH procedure is possible to recognize tumor-specific abnormality. Its applications are limited to designed probe type.
Natalia Magdalena Chrzanowska, J. Kowalewski, M. Lewandowska   +2 more
semanticscholar   +1 more source

Detection of HPV and Human Chromosome Sites by Dual-Color Fluorescence In Situ Hybridization Reveals Recurrent HPV Integration Sites and Heterogeneity in Cervical Cancer

Frontiers in Oncology, 2021
Human papillomavirus (HPV) integration in the human genome is suggested to be an important cause of cervical cancer. With the development of sequencing technologies, an increasing number of integration “hotspots” have been identified.
Jinfeng Xiong, Jing Cheng, Hui Shen, Ci Ren, Liming Wang, C. Gao, T. Zhu, Xiaomin Li, W. Ding, Da Zhu, Hui Wang   +10 more
semanticscholar   +1 more source

A technical review and guide to RNA fluorescence in situ hybridization

PeerJ, 2020
RNA-fluorescence in situ hybridization (FISH) is a powerful tool to visualize target messenger RNA transcripts in cultured cells, tissue sections or whole-mount preparations.
Alexander P. Young, D. Jackson, R. Wyeth
semanticscholar   +1 more source

Fluorescence In Situ Hybridization [PDF]

Nature Methods, 2005
Dr. Seuss’s eloquent “One FISH, two FISH, red FISH, blue FISH” (1) could have been describing one of the most significant advancements in clinical cytogenetics, fluorescence in situ hybridization (FISH). The process, as described by Pinkel et al. in 1988 (2), involved fluorescent detection of probe DNA hybridized to chromosomal target sequences.
Daynna J. Wolff, Stuart Schwartz
  +4 more sources