Results 1 to 10 of about 2,143,309 (303)

Thermodynamics and kinetics guided probe design for uniformly sensitive and specific DNA hybridization without optimization

Nature Communications, 2019
Optimisation of nucleic acid probes and blocker strands can be laborious. Here the authors construct a theoretical model of competitive DNA hybridisation to design DNA probes for optimisation-free mutation detection.
Xin Chen, Na Liu, Liquan Liu, Wei Chen, Na Chen, Meng Lin, Jiaju Xu, Xing Zhou, Hongbo Wang, Meiping Zhao, Xianjin Xiao   +10 more
doaj   +1 more source

Changes in the subgingival biofilm composition after coronally positioned flap

Journal of Applied Oral Science, 2011
OBJECTIVES: This study evaluated the effects of coronally positioned flap (CPF) on the subgingival biofilm composition. MATERIAL AND METHODS: Twenty-two subjects with gingival recessions were treated with CPF. Clinical parameters were assessed before and
Jadson Almeida Lima, Vanessa Renata Santos, Magda Feres, Luciene Cristina de Figueiredo, Poliana Mendes Duarte   +4 more
doaj   +1 more source

A Feature Selection Algorithm to Compute Gene Centric Methylation from Probe Level Methylation Data. [PDF]

PLoS ONE, 2016
DNA methylation is an important epigenetic event that effects gene expression during development and various diseases such as cancer. Understanding the mechanism of action of DNA methylation is important for downstream analysis.
Brittany Baur, Serdar Bozdag
doaj   +1 more source

DNA Stable-Isotope Probing (DNA-SIP) [PDF]

Journal of Visualized Experiments, 2010
DNA stable-isotope probing (DNA-SIP) is a powerful technique for identifying active microorganisms that assimilate particular carbon substrates and nutrients into cellular biomass. As such, this cultivation-independent technique has been an important methodology for assigning metabolic function to the diverse communities inhabiting a wide range of ...
Eric A, Dunford, Josh D, Neufeld
openaire   +2 more sources

Imaging Unique DNA Sequences in Individual Cells Using a CRISPR-Cas9-Based, Split Luciferase Biosensor

Frontiers in Genome Editing, 2022
An extensive arsenal of biosensing tools has been developed based on the clustered regularly interspaced short palindromic repeat (CRISPR) platform, including those that detect specific DNA sequences both in vitro and in live cells.
Nicholas G. Heath, Nicholas G. Heath, Nicholas G. Heath, Henriette O’Geen, Henriette O’Geen, Nicole B. Halmai, Nicole B. Halmai, Jacob E. Corn, Jacob E. Corn, David J. Segal, David J. Segal, David J. Segal   +11 more
doaj   +1 more source

Real-Time PCR Detection of Bacillus anthracis by Lambda_Ba03 Prophage Genes

Проблемы особо опасных инфекций, 2022
The aim of the study was to develop a set of primers and fluorescent probes for the detection of two chromosomal targets of Bacillus anthracis using real-time PCR based on the lambda_Ba03 prophage genes.Materials and methods.
A. S. Nizkorodova, E. R. Mal’tseva, Zh. A. Berdygulova, D. A. Naizabaeva, S. A. Kuatbekova, A. V. Zhigailov, N. Abdolla, A. S. Mashzhan, I. A. Akhmetollaev, Yu. A. Skiba, S. M. Mamadaliev   +10 more
doaj   +1 more source

Probing for DNA methylation with a voltammetric DNA detector [PDF]

The Analyst, 2014
Label-free electrochemical method for the detection of prostate cancer using a DNA biomarker.
Amir, Saheb, Stephanie, Patterson, Mira, Josowicz   +2 more
openaire   +2 more sources

Comparative evaluation of DNA and RNA probes for capture-based mitochondrial DNA next-generation sequencing

BMC Biology
Background Probe-based liquid-phase hybridization capture is a powerful and commonly used approach for next-generation sequencing (NGS) of mitochondrial DNA (mtDNA).
Tianlei Sun, Shengjing Li, Yang Liu, Kaixiang Zhou, Jiamin Wang, Zhangwen Lei, Xu Guo, Jinliang Xing, Wenjie Guo   +8 more
doaj   +1 more source

Protocols for 16S rDNA Array Analyses of Microbial Communities by Sequence-Specific Labeling of DNA Probes

The Scientific World Journal, 2003
Analyses of complex microbial communities are becoming increasingly important. Bottlenecks in these analyses, however, are the tools to actually describe the biodiversity.
Knut Rudi, Janneke Treimo, Hilde Nissen, Gerd Vegarud   +3 more
doaj   +1 more source

Quantitative assessment of LASSO probe assembly and long-read multiplexed cloning

BMC Biotechnology, 2019
Background Long Adapter Single-Stranded Oligonucleotide (LASSO) probes were developed as a novel tool for massively parallel cloning of kilobase-long genomic DNA sequences. LASSO dramatically improves the capture length limit of current DNA padlock probe
Syukri Shukor, Alfred Tamayo, Lorenzo Tosi, H. Benjamin Larman, Biju Parekkadan   +4 more
doaj   +1 more source