Results 1 to 10 of about 473,267 (264)

Comparison of Single-Stranded DNA Probes Conjugated with Magnetic Particles for Trans-Cleavage in Cas12a-Based Biosensors [PDF]

open access: yesBiosensors, 2023
Biosensors based on endonuclease Cas12 provide high specificity in pathogen detection. Sensitive detection using Cas12-based assays can be achieved using trans-cleaved DNA probes attached to simply separated carriers, such as magnetic particles (MPs ...
Aleksandr V. Ivanov   +4 more
doaj   +2 more sources

Label-free, ultra-low detection limit DNA biosensor using high quality optical microcavity functionalized by DNA tetrahedral nanostructure probes

open access: yesNanophotonics, 2023
This study proposes and demonstrates a novel label-free DNA biosensor using high quality optical microcavity functionalized by 3D DNA nanostructure probes. To achieve ultra-low limit DNA biosensing, optical sensing interface of the hollow-core, thin wall-
Wan Hongdan   +6 more
doaj   +2 more sources

Hapten-labeled DNA probes can be stored and used in fluorescence in situ hybridization for decades [PDF]

open access: yesFrontiers in Genetics
In molecular cytogenetics, fluorescence in situ hybridization (FISH) is the main technique used. In both research and diagnostics, FISH depends on well-defined and mapped DNA probes that produce brilliant signals with minimal background, visible in ...
Thomas Liehr   +2 more
doaj   +2 more sources

Embryo sexing methods in bovine and its application in animal breed

open access: yesJournal of Animal Reproduction and Biotechnology, 2022
The ability to determine the sex of bovine embryos before the transfer is advantageous in livestock management, especially in dairy production, where female calves are preferred in milk industry.
Shelema Kelbessa Bora
doaj   +1 more source

Factors Impacting Invader-Mediated Recognition of Double-Stranded DNA

open access: yesMolecules, 2022
The development of chemically modified oligonucleotides enabling robust, sequence-unrestricted recognition of complementary chromosomal DNA regions has been an aspirational goal for scientists for many decades.
Caroline P. Shepard   +3 more
doaj   +1 more source

Probing the Mechanical Unzipping of DNA [PDF]

open access: yesPhysical Review Letters, 2006
A study of the micromechanical unzipping of DNA in the framework of the Peyrard-Bishop-Dauxois model is presented. We introduce a Monte Carlo technique that allows accurate determination of the dependence of the unzipping forces on unzipping speed and temperature. Our findings agree quantitatively with experimental results for homogeneous DNA, and for $
Voulgarakis, N. K.   +3 more
openaire   +3 more sources

Probing Allostery Through DNA [PDF]

open access: yesScience, 2013
Allostery Across DNA Proteins, such as transcription factors and RNA polymerase, bind close to each other on DNA and their function is coordinated. Kim et al. (p.
Kim, Sangjin   +12 more
openaire   +4 more sources

Regions enriched for DNA repeats in chromosomes of Macrostomum mirumnovem, a species with a recent Whole Genome Duplication

open access: yesВавиловский журнал генетики и селекции, 2020
The free-living flatworm Macrostomum mirumnovem is a neopolyploid species whose genome underwent a recent Whole Genome Duplication (WGD). In the result of chromosome fusions of the ancient haploid chromosome set, large metacentric chromosomes were formed.
K. S. Zadesenets, N. B. Rubtsov
doaj   +1 more source

A Bioorthogonal Double Fluorogenic Probe to Visualize Protein–DNA Interaction

open access: yesChemosensors, 2022
Two sets of bioorthogonally applicable, double fluorogenic probes, capable of sensing DNA–protein interactions, were prepared by installing an azide or tetrazine motif onto structurally fluorogenic, DNA sensitive frames.
Attila Kormos   +7 more
doaj   +1 more source

Discovered Key CpG Sites by Analyzing DNA Methylation and Gene Expression in Breast Cancer Samples

open access: yesFrontiers in Cell and Developmental Biology, 2022
Breast cancer is the most common cancer in the world, and DNA methylation plays a key role in the occurrence and development of breast cancer. However, the effect of DNA methylation in different gene functional regions on gene expression and the effect ...
Yan-Ni Cao   +3 more
doaj   +1 more source

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