A novel cassette method for probe evaluation in the designed biochips. [PDF]
PLoS ONE, 2014 A critical step in biochip design is the selection of probes with identical hybridisation characteristics. In this article we describe a novel method for evaluating DNA hybridisation probes, allowing the fine-tuning of biochips, that uses cassettes with ...
Vitaly Zinkevich +7 more
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Comparison of Single-Stranded DNA Probes Conjugated with Magnetic Particles for Trans-Cleavage in Cas12a-Based Biosensors [PDF]
Biosensors, 2023 Biosensors based on endonuclease Cas12 provide high specificity in pathogen detection. Sensitive detection using Cas12-based assays can be achieved using trans-cleaved DNA probes attached to simply separated carriers, such as magnetic particles (MPs ...
Aleksandr V. Ivanov +4 more
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Nanophotonics, 2023 This study proposes and demonstrates a novel label-free DNA biosensor using high quality optical microcavity functionalized by 3D DNA nanostructure probes. To achieve ultra-low limit DNA biosensing, optical sensing interface of the hollow-core, thin wall-
Wan Hongdan +6 more
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Biosensing using hairpin DNA probes [PDF]
Reviews in Analytical Chemistry, 2015 Hairpin DNA probes (HDPs) are specially designed single-stranded DNA and have excellent sensing specificity. The past decade has witnessed the fast development of HDP-based biosensors due to the tremendous applications in biology, medicine, environmental
Huang Jiahao, Wu Jueqi, Li Zhigang
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Hapten-labeled DNA probes can be stored and used in fluorescence in situ hybridization for decades [PDF]
Frontiers in GeneticsIn molecular cytogenetics, fluorescence in situ hybridization (FISH) is the main technique used. In both research and diagnostics, FISH depends on well-defined and mapped DNA probes that produce brilliant signals with minimal background, visible in ...
Thomas Liehr +2 more
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A Feature Selection Algorithm to Compute Gene Centric Methylation from Probe Level Methylation Data. [PDF]
PLoS ONE, 2016 DNA methylation is an important epigenetic event that effects gene expression during development and various diseases such as cancer. Understanding the mechanism of action of DNA methylation is important for downstream analysis.
Brittany Baur, Serdar Bozdag
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Evaluation of dual-labeled fluorescent DNA probe purity versus performance in real-time PCR
BioTechniques, 2004 Real-time PCR technology using dual-labeled fluorescent oligonucleotide probes allows for sensitive, specific, and quantitative determination of mRNA or DNA targets.
Anthony T. Yeung +3 more
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LNA probes substantially improve the detection of bacterial endosymbionts in whole mount of insects by fluorescent
BMC Microbiology, 2012 Background Detection of unculturable bacteria and their localization in the host, by fluorescent in-situ hybridization (FISH), is a powerful technique in the study of host-bacteria interaction.
Priya Natarajan +2 more
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Embryo sexing methods in bovine and its application in animal breed
Journal of Animal Reproduction and Biotechnology, 2022 The ability to determine the sex of bovine embryos before the transfer is advantageous in livestock management, especially in dairy production, where female calves are preferred in milk industry.
Shelema Kelbessa Bora
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Factors Impacting Invader-Mediated Recognition of Double-Stranded DNA
Molecules, 2022 The development of chemically modified oligonucleotides enabling robust, sequence-unrestricted recognition of complementary chromosomal DNA regions has been an aspirational goal for scientists for many decades.
Caroline P. Shepard +3 more
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