Results 31 to 40 of about 2,143,309 (303)

Robust detection and identification of multiple oomycetes and fungi in environmental samples by using a novel cleavable padlock probe-based ligation-detection assay [PDF]

open access: yes, 2009
Simultaneous detection and identification of multiple pathogenic microorganisms in complex environmental samples are required in numerous diagnostic fields.
Dullemans, A.M.   +10 more
core   +2 more sources

Mixed-Sequence Recognition of Double-Stranded DNA Using Enzymatically Stable Phosphorothioate Invader Probes

open access: yesMolecules, 2015
Development of probes that allow for sequence-unrestricted recognition of double-stranded DNA (dsDNA) continues to attract much attention due to the prospect for molecular tools that enable detection, regulation, and manipulation of genes.
Brooke A. Anderson   +2 more
doaj   +1 more source

Quantitative Analysis of Nucleic Acid Hybridization on Magnetic Particles and Quantum Dot-Based Probes

open access: yesSensors, 2009
In the present study we describe sandwich design hybridization probes consisting of magnetic particles (MP) and quantum dots (QD) with target DNA, and their application in the detection of avian influenza virus (H5N1) sequences. Hybridization of 25-, 40-,
Alexey Dan Chin Yu   +4 more
doaj   +1 more source

Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling

open access: yesBioTechniques, 2004
Fluorescent nucleic acid hybridization probes traditionally have been generated by enzymatic incorporation of dye-labeled nucleotides, even though incorporation efficiency is low and variable from dye to dye.
W. Gregory Cox, Victoria L. Singer
doaj   +1 more source

DNA probe for Aeromonas salmonicida [PDF]

open access: yesApplied and Environmental Microbiology, 1992
A DNA fragment that is specific to Aeromonas salmonicida has been isolated from a genomic DNA library by differential hybridization. The specificity of this fragment as a DNA probe for A. salmonicida was shown by hybridization against reference strains and clinical isolates of A. salmonicida, related aeromonads, and species from several other bacterial
M, Hiney   +5 more
openaire   +2 more sources

DNA-Templated Timer Probes for Multiplexed Sensing

open access: yes, 2020
Simultaneous analysis based on encoded fluorophores suffers from potential crosstalk between fluorophores and the limited number of colors that can be practically resolved.
Qianqian Han (371914)   +11 more
core   +1 more source

DNA tetrahedron for fluorescence anisotropy of intercalators

open access: yesHe jishu, 2021
BackgroundThe fluorescence anisotropy of intercalative dye plays important roles in nucleic acid analysis, the construction of fluorescent probes, and biosensing.
HUANG Qiuling   +4 more
doaj   +1 more source

Single-Labeled Oligonucleotides Showing Fluorescence Changes upon Hybridization with Target Nucleic Acids

open access: yesMolecules, 2018
Sequence-specific detection of nucleic acids has been intensively studied in the field of molecular diagnostics. In particular, the detection and analysis of single-nucleotide polymorphisms (SNPs) is crucial for the identification of disease-causing ...
Gil Tae Hwang
doaj   +1 more source

DNA Probes Using Fluorescence Resonance Energy Transfer (FRET): Designs and Applications

open access: yesBioTechniques, 2001
Fluorescence resonance energy transfer (FRET) is widely used in biomedical research as a reporter method. Oligonucleotides with a DNA backbone and one or several chromophore tags have found multiple applications as FRET probes.
Vladimir V. Didenko
doaj   +1 more source

Photoactive metal complexes that bind DNA and other biomolecules as cell probes, therapeutics, and theranostics [PDF]

open access: yes, 2020
This review discusses the advantages of using luminescent d6-transition-metal complexes as cell probes for optical microscopy. In particular it focusses on the Thomas group's use of specific complexes as “building blocks” toward the construction of ...
Sreedharan, S.   +5 more
core   +1 more source

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