Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification [PDF]
, 1992 Alu-PCR protocols were optimized for the generation of human DNA probes from yeast strains containing yeast artificial chromosomes (YACs) with human inserts between 100 and 800 kb in size.
Brooks-Wilson +15 more
core +1 more source
Electrically Guided DNA Immobilization and Multiplexed DNA Detection with Nanoporous Gold Electrodes. [PDF]
, 2018 Molecular diagnostics have significantly advanced the early detection of diseases, where the electrochemical sensing of biomarkers (e.g., DNA, RNA, proteins) using multiple electrode arrays (MEAs) has shown considerable promise.
Daggumati, Pallavi +3 more
core +3 more sources
Detection of fluorescence in situ hybridization on human metaphase chromosomes by near-field scanning optical microscopy [PDF]
, 1995 Fluorescence in situ hybridization signals on human metaphase chromosomes are detected by a near-field scanning optical microscope. This makes it possible to localize and identify several fluorescently labeled genomic DNA fragments on a single ...
Jalocha, A. +5 more
core +8 more sources
Electrochemical detection of the toxic dinoflagellate Alexandrium ostenfeldii with a DNA-biosensor [PDF]
, 2005 The steady rise of observations of harmful or toxic algal blooms throughout the world in the past decades constitute a menace for coastal ecosystems and human interests.
Huljic, S. +3 more
core +1 more source
Furan-PNA : a mildly inducible irreversible interstrand crosslinking system targeting single and double stranded DNA [PDF]
, 2016 We here report on the design and synthesis of tailor-made furan-modified peptide nucleic acid (PNA) probes for covalent targeting of single stranded DNA through a crosslinking strategy.
Corradini, Roberto +3 more
core +2 more sources
Molecules, 2015 Development of probes that allow for sequence-unrestricted recognition of double-stranded DNA (dsDNA) continues to attract much attention due to the prospect for molecular tools that enable detection, regulation, and manipulation of genes.
Brooke A. Anderson +2 more
doaj +1 more source
A cellulose-based bioassay for the colorimetric detection of pathogen DNA [PDF]
, 2014 Cellulose-paper-based colorimetric bioassays may be used at the point of sampling without sophisticated equipment. This study reports the development of a colorimetric bioassay based on cellulose that can detect pathogen DNA.
Goyal, Madhu +3 more
core +1 more source
Fluorescent Probes of DNA Repair [PDF]
ACS Chemical Biology, 2017 DNA repair is now understood to play a key role in a variety of disease states, most notably cancer. Tools for studying DNA have typically relied on traditional biochemical methods which are often laborious and indirect. Efforts to study the biology and therapeutic relevance of DNA repair pathways can be limited by such methods.
David L. Wilson, Eric T. Kool
openaire +2 more sources
Sensors, 2009 In the present study we describe sandwich design hybridization probes consisting of magnetic particles (MP) and quantum dots (QD) with target DNA, and their application in the detection of avian influenza virus (H5N1) sequences. Hybridization of 25-, 40-,
Alexey Dan Chin Yu +4 more
doaj +1 more source
Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
BioTechniques, 2004 Fluorescent nucleic acid hybridization probes traditionally have been generated by enzymatic incorporation of dye-labeled nucleotides, even though incorporation efficiency is low and variable from dye to dye.
W. Gregory Cox, Victoria L. Singer
doaj +1 more source