Results 21 to 30 of about 473,267 (264)

DNA Probes Using Fluorescence Resonance Energy Transfer (FRET): Designs and Applications

open access: yesBioTechniques, 2001
Fluorescence resonance energy transfer (FRET) is widely used in biomedical research as a reporter method. Oligonucleotides with a DNA backbone and one or several chromophore tags have found multiple applications as FRET probes.
Vladimir V. Didenko
doaj   +1 more source

Biosensing using hairpin DNA probes

open access: yesReviews in Analytical Chemistry, 2015
Hairpin DNA probes (HDPs) are specially designed single-stranded DNA and have excellent sensing specificity. The past decade has witnessed the fast development of HDP-based biosensors due to the tremendous applications in biology, medicine, environmental
Huang Jiahao, Wu Jueqi, Li Zhigang
doaj   +1 more source

Single-Labeled Oligonucleotides Showing Fluorescence Changes upon Hybridization with Target Nucleic Acids

open access: yesMolecules, 2018
Sequence-specific detection of nucleic acids has been intensively studied in the field of molecular diagnostics. In particular, the detection and analysis of single-nucleotide polymorphisms (SNPs) is crucial for the identification of disease-causing ...
Gil Tae Hwang
doaj   +1 more source

An inexpensive and simple method for thermally stable immobilization of DNA on an unmodified glass surface: UV linking of poly(T)10-poly(C)10–tagged DNA probes

open access: yesBioTechniques, 2008
Microarrays printed on glass slides are often constructed by covalently linking modified oligonucleotide probes to a derivatized surface at considerable expense.
Haukur Gudnason   +3 more
doaj   +1 more source

Evaluation of dual-labeled fluorescent DNA probe purity versus performance in real-time PCR

open access: yesBioTechniques, 2004
Real-time PCR technology using dual-labeled fluorescent oligonucleotide probes allows for sensitive, specific, and quantitative determination of mRNA or DNA targets.
Anthony T. Yeung   +3 more
doaj   +1 more source

Long Oligonucleotide Arrays on Nylon for Large-Scale Gene Expression Analysis

open access: yesBioTechniques, 2002
To date, most studies of multigenic expression patterns by long DNA array have used DNA fragments as probes. These probes are usually obtained as PCR products, and this represents a time-consuming and error-prone approach, requiring strict quality ...
M. El Atifi   +5 more
doaj   +1 more source

Probing for DNA methylation with a voltammetric DNA detector [PDF]

open access: yesThe Analyst, 2014
Label-free electrochemical method for the detection of prostate cancer using a DNA biomarker.
Amir, Saheb   +2 more
openaire   +2 more sources

A Bibliometric Analysis of Publications in Uremic Toxins From 1991 to 2024

open access: yesTherapeutic Apheresis and Dialysis, EarlyView.
ABSTRACT Background Uremic toxins are a growing area of research in nephrology, with significant implications in the progression and treatment of chronic kidney disease (CKD) and the management of end‐stage kidney disease (ESKD). This bibliometric analysis aims to evaluate the global research trends, key contributors, and the impact of publications in ...
Yuh‐Shan Ho   +7 more
wiley   +1 more source

Use of a Multiplexed CMOS Microarray to Optimize and Compare Oligonucleotide Binding to DNA Probes Synthesized or Immobilized on Individual Electrodes

open access: yesSensors, 2010
The CombiMatrix microarray with 12,544 electrodes supports in situ electrochemical synthesis of user-defined DNA probes. As an alternative, we immobilized commercially synthesized DNA probes on individual electrodes coated with electropolymerized ...
David Danley   +5 more
doaj   +1 more source

DNA Stable-Isotope Probing (DNA-SIP) [PDF]

open access: yesJournal of Visualized Experiments, 2010
DNA stable-isotope probing (DNA-SIP) is a powerful technique for identifying active microorganisms that assimilate particular carbon substrates and nutrients into cellular biomass. As such, this cultivation-independent technique has been an important methodology for assigning metabolic function to the diverse communities inhabiting a wide range of ...
Eric A, Dunford, Josh D, Neufeld
openaire   +2 more sources

Home - About - Disclaimer - Privacy