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Enhancing brain image quality with 3D U-net for stripe removal in light sheet fluorescence microscopy. [PDF]
Brain InformLi C, Li Y, Zhao H, Ding L.
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Light sheet fluorescence microscopy: Advancing biological discovery with more dimensions, higher speed, and lower phototoxicity. [PDF]
Innovation (Camb)Zhou Y, Mao S, Fei P.
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Fluorescence tissue microscopy
Progress in Biophysics and Molecular Biology, 2022The increasing need to investigate large biological specimens like whole tissues in 3D has driven the development of novel optical techniques capable of volumetric imaging with no compromises in terms of field-of-view and spatial resolution. On the other hand, a major challenge in the imaging of bulk biological tissues is their strong scattering which ...
Sacconi, L +3 more
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Nature Methods, 2005
Although fluorescence microscopy permeates all of cell and molecular biology, most biologists have little experience with the underlying photophysical phenomena. Understanding the principles underlying fluorescence microscopy is useful when attempting to solve imaging problems.
Jeff W, Lichtman, José-Angel, Conchello
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Although fluorescence microscopy permeates all of cell and molecular biology, most biologists have little experience with the underlying photophysical phenomena. Understanding the principles underlying fluorescence microscopy is useful when attempting to solve imaging problems.
Jeff W, Lichtman, José-Angel, Conchello
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2019
In this two-part chapter, the background to confocal microscopy and two-photon fluorescence microscopy is first presented, with a detailed description of the optical setup. This is followed by a critical account of the many super-resolution techniques: coordinated stochastic fluorescence microscopy (photoactivation localization microscopy ...
Alberto Diaspro +7 more
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In this two-part chapter, the background to confocal microscopy and two-photon fluorescence microscopy is first presented, with a detailed description of the optical setup. This is followed by a critical account of the many super-resolution techniques: coordinated stochastic fluorescence microscopy (photoactivation localization microscopy ...
Alberto Diaspro +7 more
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2013
Optical microscopy has developed as an indispensable tool for Arabidopsis cell biology. This is due to the high sensitivity, good spatial resolution, minimal invasiveness, and availability of autofluorescent proteins, which can be specifically fused to a distinct protein of interest.
Peter, Sébastien +2 more
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Optical microscopy has developed as an indispensable tool for Arabidopsis cell biology. This is due to the high sensitivity, good spatial resolution, minimal invasiveness, and availability of autofluorescent proteins, which can be specifically fused to a distinct protein of interest.
Peter, Sébastien +2 more
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Current Protocols in Immunology, 1998
AbstractAbsorption of energy as light by some molecules and emission of energy as fluorescence can occur only at certain wavelengths, which are characteristic for a given molecule (fluorophore). Fluorescence microscopes are equipped to observe the fluorescence of one or more specific fluorophores to localize specific molecules and analyzed cellular ...
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AbstractAbsorption of energy as light by some molecules and emission of energy as fluorescence can occur only at certain wavelengths, which are characteristic for a given molecule (fluorophore). Fluorescence microscopes are equipped to observe the fluorescence of one or more specific fluorophores to localize specific molecules and analyzed cellular ...
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Fluorescent Speckle Microscopy
Cold Spring Harbor Protocols, 2011INTRODUCTIONFluorescent speckle microscopy (FSM) is a live imaging and quantitative measurement technique used for analyzing motion and turnover of macromolecular assemblies in vivo and in vitro. It differs from related imaging techniques such as photobleaching and photoactivation in its use of substantially lower concentrations of fluorescently ...
Benjamin R. Houghtaling +2 more
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Fundamentals of Fluorescence and Fluorescence Microscopy
2003This chapter discusses the fundamental physics of fluorescence. The application of fluorescence to microscopy represents an important transition in the development of microscopy, particularly as it applies to biology. It enables quantitating the amounts of specific molecules within a cell, determining whether molecules are complexing on a molecular ...
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Factors in Fluorescence Microscopy
Journal of the Optical Society of America, 1947This paper is concerned with a presentation and an attempted evaluation of the various factors governing successful observations with the microscope using fluorescence technics. The primary considerations are: (1) brightness of the fluorescent image in absolute terms and (2) brightness level, image contrast and resolving power of the eye.
Charles Sheard, D. H. Hamly
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