The analysis of fluorophore orientation by multiphoton fluorescence microscopy [PDF]
, 2010 The accessibility of tunable, ultrafast laser sources has spurred the development and wide application of specialized microscopy techniques based on chromophore fluorescence following two- and three-photon absorption.
Andrews, D.L., Leeder, J.M.
core +1 more source
Three dimensional blind image deconvolution for fluorescence microscopy using generative adversarial networks [PDF]
, 2019 Due to image blurring image deconvolution is often used for studying biological structures in fluorescence microscopy. Fluorescence microscopy image volumes inherently suffer from intensity inhomogeneity, blur, and are corrupted by various types of noise which exacerbate image quality at deeper tissue depth.
arxiv +1 more source
Simultaneous spatiotemporal super-resolution and multi-parametric fluorescence microscopy
Nature Communications, 2021 Super-resolution microscopy and single molecule fluorescence spectroscopy require optimisation of the temporal or spatial resolution, which are usually mutually exclusive.
Jagadish Sankaran +5 more
doaj +1 more source
Developing Digital Photomicroscopy
Cells, 2022 (1) The need for efficient ways of recording and presenting multicolour immunohistochemistry images in a pioneering laboratory developing new techniques motivated a move away from photography to electronic and ultimately digital photomicroscopy.
Kingsley Micklem
doaj +1 more source
Fluorescence confocal microscopy for pathologists [PDF]
Modern Pathology, 2014 Confocal microscopy is a non-invasive method of optical imaging that may provide microscopic images of untreated tissue that correspond almost perfectly to hematoxylin- and eosin-stained slides. Nowadays, following two confocal imaging systems are available: (1) reflectance confocal microscopy, based on the natural differences in refractive indices of ...
Ragazzi M. +7 more
openaire +5 more sources
In vivo endoscopic autofluorescence microspectro-imaging of bronchi and alveoli [PDF]
, 2008 Fibered confocal fluorescence microscopy (FCFM) is a new technique that can be used during a bronchoscopy to analyze the nature of the human bronchial and alveolar mucosa fluorescence microstructure. An endoscopic fibered confocal fluorescence microscopy
Bourg-Heckly, G. +3 more
core +2 more sources
Single-Molecule Clustering for Super-Resolution Optical Fluorescence Microscopy
Photonics, 2021 Molecular assembly in a complex cellular environment is vital for understanding underlying biological mechanisms. Biophysical parameters (such as single-molecule cluster density, cluster-area, pairwise distance, and number of molecules per cluster ...
Prakash Joshi, Partha Pratim Mondal
doaj +1 more source
Wavefront shaping of a Bessel light field enhances light sheet microscopy with scattered light [PDF]
, 2014 The project was supported by the UK Engineering and Physical Sciences Research Council, RS MacDonald Charitable Trust, SULSA, and the St. Andrews 600th anniversary BRAINS appeal. K. D.
Dholakia, K. +4 more
core +2 more sources
Likelihood inference for particle location in fluorescence microscopy
, 2010 We introduce a procedure to automatically count and locate the fluorescent particles in a microscopy image. Our procedure employs an approximate likelihood estimator derived from a Poisson random field model for photon emission.
Fricks, John +2 more
core +1 more source
Microfabricated rubber microscope using soft solid immersion lenses [PDF]
, 2006 We show here a technique of soft lithography to microfabricate efficient solid immersion lenses (SIL) out of rubber elastomers. The light collection efficiency of a lens system is described by its numerical aperture (NA), and is critical for applications
Gambin, Yann +2 more
core +1 more source