Results 81 to 90 of about 769,414 (303)

Real-Time analysis and visualization for single-molecule based super-resolution microscopy

, 2013
Accurate multidimensional localization of isolated fluorescent emitters is a time consuming process in single-molecule based super-resolution microscopy.
Jean-Baptiste Sibarita, Kechkar, Adel, Choquet, Daniel, Deepak Nair, Mike Heilemann, Nair, Deepak, Daniel Choquet, Sibarita, Jean-Baptiste, Adel Kechkar, Heilemann, Mike   +9 more
core   +1 more source

Engineered extracellular vesicles enriched with the miR‐214/199a cluster enhance the efficacy of chemotherapy in ovarian cancer

Molecular Oncology, EarlyView.
Loss of the miR‐214/199a cluster is associated with recurrence in ovarian cancer. Engineered small extracellular vesicles (m214‐sEVs) elevate miR‐214‐3p/miR‐199a‐5p in tumor cells, suppress β‐catenin, TLR4, and YKT6 signaling, reprogram tumor‐derived sEV cargo, reduce chemoresistance and migration, and enhance carboplatin efficacy and survival in ...
Weida Wang, Ayesha Alvero, Yi Qin, Mingjin Wang, Alexandra Fox, Yanfeng Li, Michael Millman, Amy Kemper, Gil Mor, Xian Shuang Liu, Michael Chopp, Zheng Gang Zhang, Yi Zhang   +12 more
wiley   +1 more source

Gold nanoparticle printed coverslips to facilitate fluorescence-TEM correlative microscopy.

, 2022
Correlative light and electron microscopy (CLEM) allows combining the advantages of fluorescence microscopy and electron microscopy for cell imaging. Rare phenomenon expressing cells can be studied by specifically tagged fluorophores with fluorescence ...
Anni Määttänen, Pekka Hänninen, Jessica M Rosenholm, Markus Peurla, Neeraj Prabhakar, Jouko Peltonen   +5 more
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Establishment of a humanized patient‐derived xenograft mouse model of high‐grade serous ovarian cancer for preclinical evaluation of combination immunotherapy

Molecular Oncology, EarlyView.
We have established a humanized orthotopic patient‐derived xenograft (Hu‐oPDX) mouse model of high‐grade serous ovarian cancer (HGSOC) that recapitulates human tumor–immune interactions. Using combined anti‐PD‐L1/anti‐CD73 immunotherapy, we demonstrate the model's improved biological relevance and enhanced translational value for preclinical ...
Luka Tandaric, Line Bjørge, Martine Rott Lode, Cecilie Fredvik Torkildsen, Pia Aehnlich, Rammah Elnour, Daniela Elena Costea, Lars Andreas Akslen, Liv Cecilie Vestrheim Thomsen, Emmet McCormack, Katrin Kleinmanns   +10 more
wiley   +1 more source

Nanometric depth resolution from multi-focal images in microscopy [PDF]

, 2011
We describe a method for tracking the position of small features in three dimensions from images recorded on a standard microscope with an inexpensive attachment between the microscope and the camera.
Dalgarno, Heather I. C., Dalgarno, Heather I.C., Dada, Adetunmise C., Greenaway, AH, Parton, Richard M., Dalgarno, H. I C, Dada, Adetunmise C, Towers, Catherine E., Gavin J. Gibson, Dalgarno, Paul A., Gibson, GJ, Paul A. Dalgarno, Adetunmise C. Dada, Richard J. Warburton, Parton, Richard M, Alan H. Greenaway, Gibson, Gavin J, Gibson, Gavin J., Dalgarno, Paul A, Warburton, RJ, Catherine E. Towers, Dalgarno, HI, Ilan Davis, Greenaway, Alan H, Davis, Ilan, Greenaway, Alan H., Parton, RM, Towers, Catherine E, Dada, AC, Dalgarno, Heather I C, Heather I. C. Dalgarno, Warburton, Richard J., Warburton, Richard J, Richard M. Parton, Dalgarno, PA, Davis, I, Towers, CE, Dalgarno, Paul A.; id_orcid   +37 more
core   +1 more source

CCDC80 suppresses high‐grade serous ovarian cancer migration via negative regulation of B7‐H3

Molecular Oncology, EarlyView.
PAX8 is a lineage‐specific master regulator of transcription in high‐grade serous ovarian cancer (HGSC) progression. We show for the first time that PAX8 facilitates proliferation and metastasis by repressing the cell autonomous tumor suppressor CCDC80 and inducing B7‐H3 expression.
Aya Saleh, Nitzan Medina‐Itzhaki, Milena Chekov, Eden Gal‐Swisa, Roba Gabesh‐Wahabi, Inbar Savyon, Inna Naroditsky, Hilary A. Kenny, Basem Fares, Lina Korsensky, Einav Girsh, Liron Berger, Ruth Perets   +12 more
wiley   +1 more source

Fluorescence-microscopy visualization of candidate polar proteins.

, 2022
Representative fluorescent microscopy images of C-terminal GFP fusions of candidate polar proteins in WT V. cholerae. p, plasmid-based expression. (A) Protein fusions showing diffuse distribution.
Lucía Torres-Sánchez (11938939), Alexis Carreaux (11938936), Ipek Altinoglu (11938930), Mickaël Poidevin (5655637), Yoshiharu Yamaichi (212855), Petya Violinova Krasteva (11938942), Guillaume Abriat (11938933)   +6 more
core   +1 more source

E2A selectively regulates TGF‐β–induced apoptosis in KRAS‐mutant non‐small cell lung cancer

Molecular Oncology, EarlyView.
Ability to induce apoptosis by TGF‐β is frequently lost in advanced lung adenocarcinoma despite intact TGF‐β signaling. We identify E2A as a mutant KRAS–dependent mediator of resistance to TGF‐β–induced apoptosis. TGF‐β induces E2A via SMAD3 in mutant KRAS cells, and E2A silencing restores apoptosis and enhances radiation response in cell lines ...
Sergei Chuikov, Shiva Krishna Katkam, Zhefan Wang, Venkateshwar G. Keshamouni   +3 more
wiley   +1 more source

Interaction of HS1BP3 with cortactin modulates TKS5 localisation, cell secretion and cancer malignancy

Molecular Oncology, EarlyView.
Here, we demonstrate that HS1BP3 interacts with Cortactin through a proline‐rich region (PRR3.1) and show that this interaction, and HS1BP3 itself, promote cancer cell proliferation and invasion. Inhibition of this interaction leads to build‐up of TKS5 in multivesicular endosomes and altered secretion of CD63 and CD9, providing an explanation for the ...
Arja Arnesen Løchen, Kristiane Søreng, Chiara Veroni, Laura Trachsel‐Moncho, Nagham Asp, Robin Gaupset, Lars Gustav Lyckander, Helene Knævelsrud, Lars Eftang, Anne Simonsen   +9 more
wiley   +1 more source

Laser-based fluorescence microscopy

, 2002
[Introduction]:Many microbiologists will be familiar with transmission fluorescence microscopy as well the improved epifluorescence microscopy. However, microbiologists are generally less familiar with confocal laser scanning microscopy (CLSM), despite ...
Learmonth, Robert P.
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