Results 71 to 80 of about 2,275,455 (289)

Real-Time analysis and visualization for single-molecule based super-resolution microscopy [PDF]

, 2013
Accurate multidimensional localization of isolated fluorescent emitters is a time consuming process in single-molecule based super-resolution microscopy.
Jean-Baptiste Sibarita, Kechkar, Adel, Choquet, Daniel, Deepak Nair, Mike Heilemann, Nair, Deepak, Daniel Choquet, Sibarita, Jean-Baptiste, Adel Kechkar, Heilemann, Mike   +9 more
core   +1 more source

Viral particle imaging by super-resolution fluorescence microscopy

Chemical Physics Impact, 2021
Fluorescence microscopy provides a facile imaging methodology and potentials for imaging viruses in vivo. However, it is limited by the diffraction limit of light microscopy which is above the size of the virus particles.
Stefania Castelletto, Alberto Boretti
doaj   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

Laser-based fluorescence microscopy

, 2002
[Introduction]:Many microbiologists will be familiar with transmission fluorescence microscopy as well the improved epifluorescence microscopy. However, microbiologists are generally less familiar with confocal laser scanning microscopy (CLSM), despite ...
Learmonth, Robert P.
core   +1 more source

Advances in Lensless Fluorescence Microscopy Design

Photonics
Lensless fluorescence microscopy (LLFM) has emerged as a promising approach for biological imaging, offering a simplified, high-throughput, portable, and cost-effective substitute for conventional microscopy techniques by removing lenses in favor of ...
Somaiyeh Khoubafarin, Edmond Kwesi Dadson, Aniruddha Ray   +2 more
doaj   +1 more source

Discerning protein pools by selective staining with self‐labeling tags

FEBS Letters, EarlyView.
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley   +1 more source

Applying two-photon excitation fluorescence lifetime imaging microscopy to study photosynthesis in plant leaves [PDF]

, 2009
This study investigates to which extent two-photon excitation (TPE) fluorescence lifetime imaging microscopy can be applied to study picosecond fluorescence kinetics of individual chloroplasts in leaves.
van Amerongen, H., Koen Broess, Amerongen, H., van, Jan Willem Borst, Borst, J.W., Broess, K., Herbert van Amerongen   +6 more
core   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

Extracting fluorescent reporter time courses of cell lineages from high-throughput microscopy at low temporal resolution [PDF]

, 2011
The extraction of fluorescence time course data is a major bottleneck in high-throughput live-cell microscopy. Here we present an extendible framework based on the open-source image analysis software ImageJ, which aims in particular at analyzing the ...
Ott Sascha, Georgy Koentges, Mike J Downey, Jeziorska, DM, Tamai T. Katherine, Downey, Mike J., Downey Mike J., T. Katherine Tamai, Keith W, Bretschneider, T, Tamai, T Katherine, Downey, Mike J, Vance, KW, Ott, S, Mike J. Downey, Koentges, Georgy, Downey, MJ, Keith W Vance, Danuta M. Jeziorska, Bretschneider Till, Vance, Keith W., Jeziorska Danuta M., Till Bretschneider, Danuta M Jeziorska, Koentges Georgy, Sascha Ott, Vance Keith W., Koentges, G, T Katherine Tamai, Jeziorska, Danuta M, Bretschneider, Till, Jeziorska, Danuta M., Vance, Keith W, Ott, Sascha, Tamai, T. Katherine, Tamai, TK   +35 more
core   +1 more source

High-Contrast Fluorescence Microscopy for a Biomolecular Analysis Based on Polarization Techniques Using an Optical Interference Mirror Slide

Biosensors, 2014
Fluorescence microscopy with an improved contrast for fluorescence images is developed using an optical interference mirror (OIM) slide, which can enhance the fluorescence from a fluorophore as a result of the double interference of the excitation light ...
Mitsuru Yasuda, Takuo Akimoto
doaj   +1 more source