Results 81 to 90 of about 1,049,609 (265)

Bayesian-based deconvolution fluorescence microscopy using dynamically updated nonparametric nonstationary expectation estimates [PDF]

open access: yesarXiv, 2015
Fluorescence microscopy is widely used for the study of biological specimens. Deconvolution can significantly improve the resolution and contrast of images produced using fluorescence microscopy; in particular, Bayesian-based methods have become very popular in deconvolution fluorescence microscopy.
arxiv  

Inducible fluorescent speckle microscopy [PDF]

open access: yesJournal of Cell Biology, 2016
The understanding of cytoskeleton dynamics has benefited from the capacity to generate fluorescent fiducial marks on cytoskeleton components. Here we show that light-induced imprinting of three-dimensional (3D) fluorescent speckles significantly improves speckle signal and contrast relative to classic (random) fluorescent speckle microscopy. We predict
Helder Maiato   +5 more
openaire   +4 more sources

Resolution and enhancement in nanoantenna-based fluorescence microscopy

open access: yes, 2009
Single gold nanoparticles can act as nanoantennas for enhancing the fluorescence of emitters in their near-fields. Here we present experimental and theoretical studies of scanning antenna-based fluorescence microscopy as a function of the diameter of the
Anger P.   +26 more
core   +1 more source

Intramolecular fluorescence correlation spectroscopy in a feedback tracking microscope [PDF]

open access: yes, 2010
We derive the statistics of the signals generated by shape fluctuations of large molecules studied by feedback tracking microscopy. We account for the influence of intramolecular dynamics on the response of the tracking system, and derive a general ...
Andersson   +31 more
core   +3 more sources

Structured illumination microscopy for dual-modality 3D sub-diffraction resolution fluorescence and refractive-index reconstruction [PDF]

open access: yes, 2017
Though structured illumination (SI) microscopy is a popular imaging technique conventionally associated with fluorescent super-resolution, recent works have suggested its applicability towards sub-diffraction coherent imaging with quantitative endogenous biological contrast.
arxiv   +1 more source

Fluorescence Microscopy: a statistics-optics perspective [PDF]

open access: yesarXiv, 2023
Fundamental properties of light unavoidably impose features on images collected using fluorescence microscopes. Modeling these features is ever more important in quantitatively interpreting microscopy images collected at scales on par or smaller than light's wavelength.
arxiv  

Q&A: Array tomography

open access: yesBMC Biology, 2018
Array tomography encompasses light and electron microscopy modalities that offer unparalleled opportunities to explore three-dimensional cellular architectures in extremely fine structural and molecular detail. Fluorescence array tomography achieves much
Stephen J Smith
doaj   +1 more source

CFP and YFP photostabilities are differentially affected by common mounting fluids [PDF]

open access: yes, 2010
The use of spectrally distinct variants of green fluorescent protein (GFP) such as cyan or yellow mutants (CFP and YFP, respectively) is very common in all different fields of life sciences, e.g.
Johannes A. Schmid
core   +1 more source

Adaptive Optimisation of Illumination Beam Profiles in Fluorescence Microscopy [PDF]

open access: yes, 2015
Wide-field fluorescence microscope techniques such as single/selective plane illumination microscope (SPIM) are typically configured to image large regions of a sample at once.
Girkin, J.M.   +4 more
core   +1 more source

High-Contrast Fluorescence Microscopy for a Biomolecular Analysis Based on Polarization Techniques Using an Optical Interference Mirror Slide

open access: yesBiosensors, 2014
Fluorescence microscopy with an improved contrast for fluorescence images is developed using an optical interference mirror (OIM) slide, which can enhance the fluorescence from a fluorophore as a result of the double interference of the excitation light ...
Mitsuru Yasuda, Takuo Akimoto
doaj   +1 more source

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