Results 11 to 20 of about 1,076,396 (297)

Polarization effects on fluorescence measurements [PDF]

open access: yesJournal of Research of the National Bureau of Standards Section A: Physics and Chemistry, 1975
Polarization effects on fluorescence measurements are a function of four independent variables. The first is F, the polarization ratio of the exciting light which reaches the sample. The second is r, the emission anisotropy of the sample, which is the polarization "response" of the sample to plane polarized exciting light.
E D, Cehelnik   +2 more
openaire   +3 more sources

Fluorescence Polarization for Monitoring Ribozyme Reactions in Real Time

open access: yesBioTechniques, 2000
Fluorescence polarization has been used recently to monitor diverse macromolecular interactions. In this report, the application of fluorescence polarization has been extended to monitor ribozyme reactions in real time.
K.K. Singh   +4 more
doaj   +2 more sources

Heterogeneous transition metal–based fluorescence polarization (HTFP) assay for probing protein interactions

open access: yesBioTechniques, 2009
Analyses of protein interactions are fundamental for the investigation of molecular mechanisms responsible for cellular processes and diseases, as well as for drug discovery in the pharmaceutical industry.
Alexander Riechers   +3 more
doaj   +2 more sources

Fluorescence-Polarization-Based Assaying of Lysozyme with Chitooligosaccharide Tracers [PDF]

open access: yesBiomolecules
Lysozyme is a well-known enzyme found in many biological fluids which plays an important role in the antibacterial protection of humans and animals. Lysozyme assays are used for the diagnosis of a number of diseases and utilized in immunohistochemistry ...
Liliya I. Mukhametova   +7 more
doaj   +2 more sources

Nanomaterials Used in Fluorescence Polarization Based Biosensors [PDF]

open access: yesInternational Journal of Molecular Sciences, 2022
Jin Zhang, Yingqi Zhang
exaly   +2 more sources

Fluorescence polarization of green fluorescence protein [PDF]

open access: yesProceedings of the National Academy of Sciences, 2002
We report here the striking anisotropy of fluorescence exhibited by crystals of native green fluorescence protein (GFP). The crystals were generated by water dialysis of highly purified GFP obtained from the jellyfish Aequorea .
Shinya, Inoué   +4 more
openaire   +3 more sources

Hyperdimensional Imaging Contrast Using an Optical Fiber

open access: yesSensors, 2021
Fluorescence properties of a molecule can be used to study the structural and functional nature of biological processes. Physical properties, including fluorescence lifetime, emission spectrum, emission polarization, and others, help researchers probe a ...
Jenu V. Chacko   +4 more
doaj   +1 more source

Advances of super-resolution fluorescence polarization microscopy and its applications in life sciences

open access: yesComputational and Structural Biotechnology Journal, 2020
Fluorescence polarization microscopy (FPM) analyzes both intensity and orientation of fluorescence dipole, and reflects the structural specificity of target molecules. It has become an important tool for studying protein organization, orientational order,
Long Chen   +6 more
doaj   +1 more source

Identification of single-point mutations in mycobacterial 16S rRNA sequences by confocal single-molecule fluorescence spectroscopy [PDF]

open access: yes, 2006
Marme N, Friedrich A, Müller M, et al. Identification of single-point mutations in mycobacterial 16S rRNA sequences by confocal single-molecule fluorescence spectroscopy. Nucleic Acids Research.
Friedrich, Achim   +7 more
core   +1 more source

Temperature measurement in microchannel liquid flow by measuring fluorescence polarization

open access: yesNihon Kikai Gakkai ronbunshu, 2017
A fluid temperature measurement technique based on fluorescence polarization is developed and applied to measure the two-dimensional temperature distributions in microchannel.
Atsushi SUZUKI   +4 more
doaj   +1 more source

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