Results 31 to 40 of about 1,076,396 (297)

Fluorescence polarization immunoassay for cortisol

Clinica Chimica Acta, 1979
A fluorescence polarization immunoassay for serum cortisol was established using cortisol 21-amine which was readily coupled with fluorescein isothiocyanate. The proposed method is sufficiently sensitive, reliable, specific and simple for routine determination of serum cortisol. The assay is rapid, without separation of antibody-bound and free ligands.
MIYAI, Kiyoshi, KOBAYASHI, Yoshiharu, TSUBOTA, Noriko, WATANABE, Fukuko   +3 more
openaire   +2 more sources

Dynamics of Fluorescence Polarization in Macromolecules [PDF]

Proceedings of the National Academy of Sciences, 1972
Reexamination of the theory of fluorescence time dependence owing to rotational diffusion of rigid macromolecules reveals deficiencies or hidden restrictions in each of the previous treatments. The correct master equation has five exponential decay terms, with preexponential factors that depend upon the diffusion constants and, in a completely ...
G G, Belford, R L, Belford, G, Weber
openaire   +2 more sources

Study on the Fluorescence Spectra Characteristics of Vinegar-Water Solutions

Journal of Spectroscopy, 2016
Fluorescence spectra and polarization spectra of vinegar-water solutions with different concentration of CH3COOH have been studied. The characteristics and mechanism of fluorescence spectra are discussed, and polarization degree is calculated.
Dan Li, Xuan Wang, Caiqin Han
doaj   +1 more source

Probing the Protein-Protein Interaction Between the ATRX_ADD Domain and the Histone H3 Tail

Molecules, 2020
While loss-of-function mutations in the ATRX gene have been implicated as a driving force for a variety of pediatric brain tumors, as well as pancreatic neuroendocrine tumors, the role of ATRX in gene regulation and oncogenic development is not well ...
Angela M. Zaino, Radha Charan Dash, M. Kyle Hadden   +2 more
doaj   +1 more source

Fluorescence polarization immunoassay of colchicine [PDF]

Journal of Pharmaceutical and Biomedical Analysis, 2018
In this study, a fluorescence polarization immunoassay (FPIA) technique was developed to determine colchicine (COL), an alkaloid of noxious plants of the order Liliales that is used in a number of medications to treat gout. An optimal combination of the polyclonal antibody and the antigen labelled with fluorescein isothiocyanate (FITC) was selected ...
Elena A. Zvereva, Anatoly V. Zherdev, Andey A. Formanovsky, Ramadan A. Abuknesha, Sergei A. Eremin, Boris B. Dzantiev   +5 more
openaire   +4 more sources

Picosecond time-resolved fluorescence of phycobiliproteins [PDF]

, 1990
The α- and β-subunits of C-phycocyanin from Mastigocladus laminosus were prepared according to revised procedures. Both subunits are isolated as dimers, which can be dissociated into monomers with detergent mixtures.
Geiselhart, P., S. Schneider, Scheer, Hugo, Fischer, R., Schneider, Siegfried, R. Fischer, H. Scheer, J. Gottstein, Gottstein, J., P. Geiselhart   +9 more
core   +1 more source

Detection of Anatoxin-a and Three Analogs in Anabaena spp. Cultures: New Fluorescence Polarization Assay and Toxin Profile by LC-MS/MS

Toxins, 2014
Anatoxin-a (ATX) is a potent neurotoxin produced by several species of Anabaena spp. Cyanobacteria blooms around the world have been increasing in recent years; therefore, it is urgent to develop sensitive techniques that unequivocally confirm the ...
Jon A. Sanchez, Paz Otero, Amparo Alfonso, Vitor Ramos, Vitor Vasconcelos, Romulo Aráoz, Jordi Molgó, Mercedes R. Vieytes, Luis M. Botana   +8 more
doaj   +1 more source

Fluorescence Anisotropy as a Temperature-Sensing Molecular Probe Using Fluorescein

Micromachines, 2021
Fluorescence anisotropy, a technique to study the folding state of proteins or affinity of ligands, is used in this present work as a temperature sensor, to measure the microfluidic temperature field, by adding fluorophore in the liquid.
Puneet Jain, Takuya Aida, Masahiro Motosuke   +2 more
doaj   +1 more source

Study on Bioagents or Bioaerosols standoff detection by lidar

MATEC Web of Conferences, 2016
Scattering, fluorescence and polarization are the important data source for bioagent or bioaerosol time-space observation and identification. This paper stated and discussed the theory and data inversion principles for Mie scattering, laser induced ...
Yang Hui, Sun Yan Fei, Wang Tie Dong, Zhao Xue Song, Dong Yan, Qing Feng   +5 more
doaj   +1 more source

Tracking multiple conformations occurring on angstrom-and-millisecond scales in single amino-acid-transporter molecules

eLife, 2023
Most membrane protein molecules undergo conformational changes as they transition from one functional state to another one. An understanding of the mechanism underlying these changes requires the ability to resolve individual conformational states, whose
Yufeng Zhou, John H Lewis, Zhe Lu
doaj   +1 more source