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Zinc‐Containing Bioactive Glass Programs Macrophage Polarization through Extracellular Traps Regulation for Enhanced Diabetic Wound Healing

Advanced Healthcare Materials, EarlyView.
Zinc‐containing bioactive glass (ZnBG) promotes diabetic wound healing by regulating macrophage extracellular traps (METs). Specifically, ZnBG reduces oxidative stress and inhibits the PAD4 and NLRP3/caspase‐1/GSDMD signaling pathways, thereby suppressing MET formation.
RuiYang Sun, XueBo Wei, ZhuoYang Song, JunJun Luo, JingYi Ye, YinNan Zhang, LiFei Zhu, Jie Gao, Hongyu Zhang, Hong Zhu, Xin Wang, Ke Xu   +11 more
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Fluorescence polarization immunoassay of ractopamine

Applied Biochemistry and Microbiology, 2016
A technique was developed for fluorescence polarization immunoassay (FPIA) of ractopamine, a toxic low molecular weight nonsteroidal growth regulator belonging to the most controlled contaminants of food products of animal origin. The assay is based on the competition between a sample containing ractopamine and ractopamine–fluorophore conjugate for ...
E A, Zvereva, N A, Shpakova, A V, Zherdev, L, Kiu, C, Xu, S A, Eremin, B B, Dzantiev   +6 more
openaire   +2 more sources

Fluorescence Polarization Anisotropy in Microdroplets

The Journal of Physical Chemistry Letters, 2018
Chemical reactions can be greatly accelerated in microdroplets, but the factors that lead to acceleration are still being elucidated. Using rhodamine 6G (R6G) as a model compound, we studied the density distribution and fluorescence polarization anisotropy of this dye in water-in-oil microdroplets.
Zhenpeng Zhou, Xin Yan, Yin-Hung Lai, Richard N. Zare   +3 more
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Fluorescence polarization immunoassay for cortisol

Clinica Chimica Acta, 1979
A fluorescence polarization immunoassay for serum cortisol was established using cortisol 21-amine which was readily coupled with fluorescein isothiocyanate. The proposed method is sufficiently sensitive, reliable, specific and simple for routine determination of serum cortisol. The assay is rapid, without separation of antibody-bound and free ligands.
Y, Kobayashi, K, Amitani, F, Watanabe, K, Miyai   +3 more
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FRET by Fluorescence Polarization Microscopy

2008
The widespread success in using genetically encoded fluorescent proteins (FPs) to track protein motion in living cells has led to extensive interest in measuring Förster resonance energy transfer (FRET) between two FPs of different colors. FRET occurs over distances less than 10-nm and can thus be used to detect protein-protein interactions and changes
David W, Piston, Mark A, Rizzo
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Stopped-flow Fluorescence Polarization Immunoassay

Combinatorial Chemistry & High Throughput Screening, 2003
A general survey of the analytical application of kinetic methodology in fluorescence polarization immunoassay (FPIA) is presented. Stopped-flow mixing technique (SF) allows the initial rate of the immunochemical reaction between the tracer and the antibody to be obtained, which is used as the analytical parameter instead of the equilibrium signal used
Agustina, Gómez-Hens, M Paz, Aguilar-Caballos   +1 more
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Biochemistry laboratory experiment: Polarized fluorescence

Journal of Chemical Education, 1968
In this experimental procedure, pyridoxamine is used to investigate some of the enzyme complexes formed by glucose dehydrogenase.
M A, Carper, W R, Carper
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Fluorescence Polarization Immunoassays for Pesticides

Combinatorial Chemistry & High Throughput Screening, 2003
Fluorescence polarization immunoassay methods for the detection of pesticides and their metabolites or degradation products are reviewed. Advantages and limitations for application to pesticide detection in environmental and food samples are discussed.
Sergei A, Eremin, David S, Smith
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High-throughput fluorescence polarization immunoassay by using a portable fluorescence polarization imaging analyzer

Lab on a Chip, 2019
A portable fluorescence polarization imaging analyzer was developed for high-throughput fluorescence polarization immunoassay.
Osamu Wakao, Ken Satou, Ayano Nakamura, Polina A. Galkina, Keine Nishiyama, Ken Sumiyoshi, Fumio Kurosawa, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Mikhail A. Proskurnin, Koji Shigemura, Akihide Hibara, Manabu Tokeshi   +13 more
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Fluorescence polarization for mycotoxin determination

Mycotoxin Research, 2006
Over the last few years several laboratories have reported fluorescence polarization (FP) immunoassays for mycotoxins. These have included assays for fumonisins, deoxynivalenol and acetylated derivatives, aflatoxins, ochratoxin A, and zearalenone and related metabolites. Sensitivity in the FP assays may change dramatically over time, depending upon the
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