Results 51 to 60 of about 121,227 (315)
Saudi Pharmaceutical Journal, 2019 A synthesized and promising biologically hypoglycemic compound 5,6-Dichloro-2-[2-(pyridin-2-yl)ethyl]isoindoline-1,3-dione (5e) was studied for its binding to a model protein (bovine serum albumin; BSA) by spectroscopic and molecular simulation ...
Mohammed M. Alanazi +5 more
doaj +1 more source
, 1999 Depending on the HIV-1 isolate, MN or BH10, the nucleocapsid protein, NCp7, corresponds to a 55- or 71-amino acid length product, respectively. The MN NCp7 contains a single Trp residue at position 37 in the distal zinc finger motif, and the BH10 NCp7 ...
Piemont, Etienne +13 more
core +1 more source
FLUORESCENCE QUENCHING-BASED IMMUNOSENSING PLATFORM FOR THE DETECTION OF CLINICALLY RELEVANT PROTEINS [PDF]
, 2023 "Immunoassays are nowadays a crucial tool for diagnostics, drug development, and environmental monitoring. However, most immunoassay methods involve cumbersome procedures such as washing, blocking, or separation steps, and in general, require many ...
Edwin Johan Ortiz Riaño
core
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Fluorescence Quenching in Model Membranes
Biophysical Journal, 1982 Fluorescence quenching of membrane-bound fluorophors by nitroxide spin-labeled phospholipids has proved to be a useful technique for examining lipid-fluorophor contact (1-3). When the fluorophor is the tryptophanyl residue of a polypeptide, this method can yield relative binding constants of phospholipids to a membrane-bound polypeptide.
openaire +2 more sources
FEBS Open Bio, EarlyView.The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer +7 more
wiley +1 more source
Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins
FEBS Open Bio, EarlyView.Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley +1 more source
, 2021 Optical sensors for fluorescence of chlorophyll a (f-Chl a) and phycocyanin (f-PC) are increasingly used as a proxy for biomass of algae and cyanobacteria, respectively. They provide measurements at high-frequency and modest cost.
Rinke, K +4 more
core +1 more source
β‐Catenin/c‐Myc Axis Modulates Autophagy Response to Different Ammonia Concentrations
Advanced Biology, Volume 9, Issue 3, March 2025.Ammonia, detoxified by the liver into urea and glutamine, impacts autophagy differently at varying levels. Low ammonia activates autophagy via c‐Myc and β‐catenin, while high levels suppress it. Using Huh7 cells and Spf‐ash mice, c‐Myc's role in cytoprotective autophagy is revealed, offering insights into hyperammonemia and potential therapeutic ...
S. Sergio +11 more
wiley +1 more source
, 1990 Kohse-Höinghaus K, Meier U, Attal-Trétout B. Laser-induced fluorescence study of OH in flat flames of 1–10 bar compared with resonance CARS experiments. Applied Optics.
Kohse-Höinghaus, Katharina +2 more
core +1 more source