Results 271 to 280 of about 41,336 (313)

Lipase Surface Diffusion Studied by Fluorescence Recovery after Photobleaching

Langmuir, 2005
We have analyzed surface diffusion properties of a variant of Thermomyces lanuginosa lipase (TLL) on hydrophilic silica and silica methylated with dichlorodimethylsilane (DDS) or octadecyltrichlorosilane (OTS). For this study a novel method for analysis of diffusion on solid surfaces was developed.
Andreas W, Sonesson, Thomas H, Callisen, Hjalmar, Brismar, Ulla M, Elofsson   +3 more
openaire   +2 more sources

Fluorescence recovery after photobleaching of suspensions of vacuoles

Biophysical Chemistry, 1996
In this work we derive theoretical expressions for the FRAP measured on a liquid suspension of vacuoles labelled by a fluorescent probe bound to the surface membrane of these vacuoles. The bleaching laser beam creates an inhomogeneity in the surface concentration of the probe molecules.
openaire   +2 more sources

Frequency Domain Analysis for Fluorescence Recovery after Photobleaching

Applied Spectroscopy, 2006
Fourier transformation is evaluated as a means of improving precision in the analysis of fluorescence-recovery-after-photobleaching (FRAP) data. Simulations of FRAP data of 2 m points, where m is an integer, are Fourier transformed to obtain the frequency domain data.
openaire   +2 more sources

Confocal Fluorescence Recovery After Photobleaching of Green Fluorescent Protein in Solution

Journal of Fluorescence, 2006
Fluorescence recovery after photobleaching (FRAP) is one of the most widely used approaches to quantitatively estimate diffusion characteristics of molecules in solution and cellular systems. In general, comparison of the diffusion times (t (1/2)) from a FRAP experiment provides qualitative estimates of diffusion rates.
Thomas J, Pucadyil, Amitabha, Chattopadhyay   +1 more
openaire   +2 more sources

Fluorescence Recovery After Photobleaching (FRAP): Acquisition, Analysis, and Applications

2014
A significant number of biological processes occur at, or involve cellular membranes, including; cell adhesion, migration, endocytosis, signal transduction, and many biochemical reactions involving membrane anchored scaffolds. Each process involves a complex arrangement of interacting molecules whose location in space and time influence the outcome of ...
Michael, Carnell, Alex, Macmillan, Renee, Whan   +2 more
openaire   +2 more sources

Optimizing Detection of Tissue Anisotropy by Fluorescence Recovery after Photobleaching

Bulletin of Mathematical Biology, 2006
Fluorescence recovery after photobleaching (FRAP) has been widely used to measure fluid flow and diffusion in gels and tissues. It has not been widely used in detection of tissue anisotropy. This may be due to a lack of applicable theory, or due to inherent limitations of the method. We discuss theoretical aspects of the relationship between anisotropy
Lubkin, S. R., Wan, X.
openaire   +3 more sources

Fluorescence Recovery after Photobleaching: Application to Nuclear Proteins

2005
Fluorescence redistribution after photobleaching (FRAP) has received increasing attention ever since it was first introduced into cell biological research. The method was developed in the 1970s, when its biological application mainly focused on the mobility of fluorescently labelled constituents of the cell membrane.
openaire   +3 more sources

Peer Reviewed: Applications of Fluorescence Recovery after Photobleaching

Analytical Chemistry, 1997
Determining the diffusion coefficients of fluorescent molecules provides details on molecular-scale structure.
John M. Kovaleski, Mary J. Wirth
openaire   +1 more source

Diffusion Measurements by Fluorescence Recovery After Photobleaching

2001
Fluorescence recovery after photobleaching (FRAP) is a classic technique for measurement of the translational diffusion of fluorophores and fluorescently labeled macromolecules. In spot photobleaching, a brief intense light pulse irreversibly bleaches fluorophores in a defined volume of a fluorescent sample. With an attenuated probe beam, the diffusion
Alan S. Verkman, Lakshmanan Vetrivel, Peter Haggie   +2 more
openaire   +1 more source

Measuring Immune Receptor Mobility by Fluorescence Recovery After Photobleaching

2011
The coordinated effort of cells in the immune system relies heavily on surface receptor interactions. Immune receptor mobility provides vital information on the function and responses of immune cells, and these measurements shed light on their interactions with other membrane, cytosolic, and extracellular matrix proteins.
Kristen, Silver, Rene E, Harrison
openaire   +2 more sources