Results 271 to 280 of about 40,790 (312)
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Frequency Domain Analysis for Fluorescence Recovery after Photobleaching
Applied Spectroscopy, 2006Fourier transformation is evaluated as a means of improving precision in the analysis of fluorescence-recovery-after-photobleaching (FRAP) data. Simulations of FRAP data of 2 m points, where m is an integer, are Fourier transformed to obtain the frequency domain data.
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Lipase Surface Diffusion Studied by Fluorescence Recovery after Photobleaching
Langmuir, 2005We have analyzed surface diffusion properties of a variant of Thermomyces lanuginosa lipase (TLL) on hydrophilic silica and silica methylated with dichlorodimethylsilane (DDS) or octadecyltrichlorosilane (OTS). For this study a novel method for analysis of diffusion on solid surfaces was developed.
Andreas W, Sonesson +3 more
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Diffusion measurements in epidermal tissues with fluorescent recovery after photobleaching
Skin Research and Technology, 2008Background/purpose: Pressure ulcers are areas of soft tissue breakdown, resulting from sustained mechanical loading of the skin and underlying tissues. Measuring biochemical markers that are released upon mechanical loading by the epidermis seems a promising method for objective risk assessment of the development of pressure ulcers.
Cornelissen, L.H. +3 more
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Analysis of Integrin Dynamics by Fluorescence Recovery After Photobleaching
2007Cell migration is a complex cellular behavior that involves the controlled reorganization of the link between the actin cytoskeleton and the extracellular matrix. This mechanical connection is provided by transmembrane receptors of the integrin family. Integrins are heterodimeric receptors that undergo an allosteric switch when activated by external or
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Fluorescence Recovery after Photobleaching: Application to Nuclear Proteins
2005Fluorescence redistribution after photobleaching (FRAP) has received increasing attention ever since it was first introduced into cell biological research. The method was developed in the 1970s, when its biological application mainly focused on the mobility of fluorescently labelled constituents of the cell membrane.
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Fluorescence Recovery after Photobleaching in Colloidal Science: Introduction and Application
ACS Biomaterials Science & Engineering, 2022FRAP (fluorescence recovery after photo bleaching) is a method for determining diffusion in material science. In industrial applications such as medications, foods, Medtech, hygiene, and textiles, the diffusion process has a substantial influence on the overall qualities of goods.
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Fluorescence Recovery after Photobleaching as a Probe of Diffusion in Starch Systems
Biomacromolecules, 2006The diffusion coefficients of dextran probes of various molecular weights in starch solutions over a wide concentration range were carried out using fluorescent recovery after photobleaching (FRAP), combined with a confocal microscope and tracer probe diffusion.
Perry, P. A. +2 more
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Diffusion Measurements by Fluorescence Recovery After Photobleaching
2001Fluorescence recovery after photobleaching (FRAP) is a classic technique for measurement of the translational diffusion of fluorophores and fluorescently labeled macromolecules. In spot photobleaching, a brief intense light pulse irreversibly bleaches fluorophores in a defined volume of a fluorescent sample. With an attenuated probe beam, the diffusion
Alan S. Verkman +2 more
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Fluorescence Recovery After Photobleaching (FRAP): Acquisition, Analysis, and Applications
2014A significant number of biological processes occur at, or involve cellular membranes, including; cell adhesion, migration, endocytosis, signal transduction, and many biochemical reactions involving membrane anchored scaffolds. Each process involves a complex arrangement of interacting molecules whose location in space and time influence the outcome of ...
Michael, Carnell +2 more
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Probing the dynamics of photosynthetic membranes with fluorescence recovery after photobleaching
Trends in Plant Science, 2002In the past few years, there has been remarkable progress in knowledge of the structures and organization of the protein complexes of photosynthetic membranes. However, static structures do not tell the whole story. Photosynthetic membranes, like other biological membranes, are dynamic systems.
Conrad W, Mullineaux, Mary, Sarcina
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