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Fluorescence recovery after photobleaching reveals the biochemistry of nucleocytoplasmic exchange
Analytical and Bioanalytical Chemistry, 2012Fluorescence recovery after photobleaching (FRAP) can help unveil subtle dynamical and biochemical properties of intracellular components. A peculiar aspect of this method is that it is based on the change of optical properties only, whereas dynamics and biochemistry of the molecules of interest are not perturbed.
Bizzarri R +3 more
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Fluorescence Recovery After Photobleaching (FRAP) with a Focus on F‐actin
Current Protocols in Neuroscience, 2012AbstractIn this unit on fluorescence recovery after photobleaching (FRAP), an imaging approach to study protein‐protein interactions in situ is described. The protocols presented use confocal microscopy to examine the mobility of a fluorescent protein by measuring the recovery of the protein in a bleached area.
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Fluorescence Recovery After Photobleaching Studies of Lipid Rafts
2007Fluorescence recovery after photobleaching (FRAP) is a microscopy-based technique that can be used to ask how lipid rafts impact protein and lipid diffusion in cells. This chapter, describes how to perform FRAP measurements of putative raft and nonraft proteins and lipids using a confocal microscope.
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Measuring Immune Receptor Mobility by Fluorescence Recovery After Photobleaching
2011The coordinated effort of cells in the immune system relies heavily on surface receptor interactions. Immune receptor mobility provides vital information on the function and responses of immune cells, and these measurements shed light on their interactions with other membrane, cytosolic, and extracellular matrix proteins.
Kristen, Silver, Rene E, Harrison
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Peer Reviewed: Applications of Fluorescence Recovery after Photobleaching
Analytical Chemistry, 1997Determining the diffusion coefficients of fluorescent molecules provides details on molecular-scale structure.
John M. Kovaleski, Mary J. Wirth
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Fluorescence recovery after photobleaching
2021Favard, Cyril, Sibarita, Jean-Baptiste
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Fluorescence Recovery After Photobleaching
Imaging & Microscopy, 2006Sarah Burrows +3 more
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