Results 41 to 50 of about 868,915 (328)
Plasmonic antennas and zero mode waveguides to enhance single molecule fluorescence detection and fluorescence correlation spectroscopy towards physiological concentrations [PDF]
WIREs Nanomed Nanobiotechnol 2014, 6, 268-282, 2014 Single-molecule approaches to biology offer a powerful new vision to elucidate the mechanisms that underpin the functioning of living cells. However, conventional optical single molecule spectroscopy techniques such as F\"orster fluorescence resonance energy transfer (FRET) or fluorescence correlation spectroscopy (FCS) are limited by diffraction to ...
arxiv +1 more source
Unraveling multi-state molecular dynamics in single-molecule FRET experiments- Part I: Theory of FRET-Lines [PDF]
, 2021 Conformational dynamics of biomolecules are of fundamental importance for their function. Single-molecule F\"orster Resonance Energy Transfer (smFRET) is a powerful approach to inform on the structure and the dynamics of labeled molecules. If the dynamics occur on the sub-millisecond timescale, capturing and quantifying conformational dynamics can be ...
arxiv +1 more source
A fluorescence resonance energy transfer activation sensor for Arf6 [PDF]
Analytical Biochemistry, 2008 The involvement of the small GTPase Arf6 in Rac activation, cell migration, and cancer invasiveness suggests that it is activated in a spatially and temporally regulated manner. Small GTPase activation has been imaged in cells using probes in which the GTPase and a fragment of a downstream effector protein are fused to fluorescent reporter proteins ...
Steven G. Sligar +5 more
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Biotechnology for Biofuels, 2008 Background Engineering microorganisms to improve metabolite flux requires detailed knowledge of the concentrations and flux rates of metabolites and metabolic intermediates in vivo.
Looger Loren L +4 more
doaj +1 more source
Application of fluorescence resonance energy transfer in protein studies [PDF]
Journal of Molecular Structure, 2014 Since the physical process of fluorescence resonance energy transfer (FRET) was elucidated more than six decades ago, this peculiar fluorescence phenomenon has turned into a powerful tool for biomedical research due to its compatibility in scale with biological molecules as well as rapid developments in novel fluorophores and optical detection ...
Linlin Ma, Fan Yang, Jie Zheng
openaire +5 more sources
DNA Probes Using Fluorescence Resonance Energy Transfer (FRET): Designs and Applications
BioTechniques, 2001 Fluorescence resonance energy transfer (FRET) is widely used in biomedical research as a reporter method. Oligonucleotides with a DNA backbone and one or several chromophore tags have found multiple applications as FRET probes.
Vladimir V. Didenko
doaj +1 more source
Advanced Data Analysis for Fluorescence-Lifetime Single-Molecule Localization Microscopy
Frontiers in Bioinformatics, 2021 Fluorescence-lifetime single molecule localization microscopy (FL-SMLM) adds the lifetime dimension to the spatial super-resolution provided by SMLM. Independent of intensity and spectrum, this lifetime information can be used, for example, to quantify ...
Jan Christoph Thiele +5 more
doaj +1 more source
Genotyping of IRGM tetranucleotide promoter oligorepeats by fluorescence resonance energy transfer
BioTechniques, 2009 Fluorescence resonance energy transfer (FRET) genotyping has been well established for the rapid assessment of single nucleotide polymorphisms (SNPs) and deletions. A design is presented that allows the typing of short tandem oligo repeat sequences using
Christopher D. Intemann +5 more
doaj +1 more source
Synthetic energy sensor AMPfret deciphers adenylate-dependent AMPK activation mechanism
Nature Communications, 2019 AMP-activated protein kinase AMPK senses and regulates cellular energy state. Here the authors engineer a synthetic sensor, AMPfret, that allows direct, real-time readout of the AMPK conformational state by fluorescence resonance energy transfer (FRET).
Martin Pelosse +6 more
doaj +1 more source
Fluorescence resonance energy transfer (FRET) sensors
BMC Pharmacology, 2008 The principle of "fluorescence resonance energy transfer" (FRET) has been exploited in cell biology to demonstrate the interaction of two proteins in living cells. FRET is highly specific and sensitive and allows for the online recording of events that modulate the interaction.
Ingrid Gsandtner +3 more
openaire +2 more sources