Results 41 to 50 of about 208,014 (290)

Some Secrets of Fluorescent Proteins: Distinct Bleaching in Various Mounting Fluids and Photoactivation of cyan fluorescent proteins at YFP-Excitation [PDF]

, 2011
Background The use of spectrally distinct variants of green fluorescent protein (GFP) such as cyan or yellow mutants (CFP and YFP, respectively) is very common in all different fields of life sciences, e.g. for marking specific proteins or cells
AF Fradkov, AG Harpur, Andreas Hofmann, C Dinant MEVRWVABH, C Thaler, DW Piston, EA Jares-Erijman, EB Van Munster, FS Wouters, FS Wouters, G Valentin, H-w Ai, J Goedhart, JA Schmid, JA Schmid, JA Schmid, JM Lingbeck, Johannes A. Schmid, K Ebner, L Abrami, M Ormo, MA Rizzo, MK Raarup, MT Kirber, Naila Malkani, NC Shaner, PI Bastiaens, PI Bastiaens, RY Tsien, SE Verrier, T Karpova, T Nagai, TB McAnaney, TW Gadella Jr   +33 more
core   +4 more sources

Synthetic energy sensor AMPfret deciphers adenylate-dependent AMPK activation mechanism

Nature Communications, 2019
AMP-activated protein kinase AMPK senses and regulates cellular energy state. Here the authors engineer a synthetic sensor, AMPfret, that allows direct, real-time readout of the AMPK conformational state by fluorescence resonance energy transfer (FRET).
Martin Pelosse, Cécile Cottet-Rousselle, Cécile M. Bidan, Aurélie Dupont, Kapil Gupta, Imre Berger, Uwe Schlattner   +6 more
doaj   +1 more source

Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions

FEBS Letters, EarlyView.
We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva, Puspa Kunwar, Md Ibnul Rifat Rahman, Joanna Koryo Kwao, Nathan Lehman, Zihan Zhang, Trenton Paul, Claire Cheng, Nicholas Truex, Hui‐Ting Lee, Jun Zhang   +10 more
wiley   +1 more source

Development of a DNA sensor using molecular logic gate

, 2013
This communication reports the increase in fluorescence resonance energy transfer (FRET) efficiency between two laser dyes in presence of Deoxyribonucleic acid (DNA).
Bhattacharjee, D., Chakraborty, S., Dey, Dibyendu, Hussain, Syed Arshad, Sinha, S.   +4 more
core   +1 more source

Interplay between RNA‐protein interactions and RNA structures in gene regulation

FEBS Open Bio, EarlyView.
Methodological advances in mapping transcriptome‐wide RNA‐protein interactions and RNA structures have started to uncover the potential of RNP conformations in gene regulation. Competing RNA–RNA, RNA‐protein and protein–protein interactions shape the compaction and function of RNPs throughout their lifetime and may provide novel therapeutic targets in ...
Jenni Rapakko, Mauro Scaravilli, Minna‐Liisa Änkö   +2 more
wiley   +1 more source

White Light Emission by Simultaneous One Pot Encapsulation of Dyes into One-Dimensional Channelled Aluminophosphate

Nanomaterials, 2020
By simultaneous occlusion of rationally chosen dyes, emitting in the blue, green and red region of the electromagnetic spectrum, into the one-dimensional channels of a magnesium-aluminophosphate with AEL-zeolitic type structure, MgAPO-11, a solid-state ...
Rebeca Sola-Llano, Ainhoa Oliden-Sánchez, Almudena Alfayate, Luis Gómez-Hortigüela, Joaquín Pérez-Pariente, Teresa Arbeloa, Johan Hofkens, Eduard Fron, Virginia Martínez-Martínez   +8 more
doaj   +1 more source

Quantitative Imaging of Protein-Protein Interactions by Multiphoton Fluorescence Lifetime Imaging Microscopy using a Streak camera

, 2003
Fluorescence Lifetime Imaging Microscopy (FLIM) using multiphoton excitation techniques is now finding an important place in quantitative imaging of protein-protein interactions and intracellular physiology.
A. Masuda, B. Herman, Becker, Clayton, Clegg, Cole, Elangovan, French, Gadella, Gerritsen, Harpur, Heya, Konig, Konig, Krishnan, Krishnan, Lakowicz, Mahajan, Neuberth, Oida, Periasamy, R. V. Krishnan, Schonle, Selvin, Squire, Squire, Straub, Sytsma, Szmacinski, Tahara, Tavare, V. E. Centonze, van Zandvoort, Verveer, Verveer, Verveer   +35 more
core   +1 more source

FÖRSTER TRANSFER CALCULATIONS BASED ON CRYSTAL STRUCTURE DATA FROM Agmenellum quadruplicatum C-PHYCOCYANIN [PDF]

, 1987
Excitation energy transfer in C-phycocyanin is modeled using the Forster inductive resonance mechanism. Detailed calculations are carried out using coordinates and orientations of the chromophores derived from X-ray crystallographic studies of C ...
Cogdell R. J., Dale R. E., Forster T., Glazer A. N., Grabowski J., Grabowski J., Hefferle P., Hefferle P., Holzwarth A. R., Holzwarth A. R., John W., Lehner H., Lundell D. J., MacColl R., Sauer K., Sauer K., Scheer H., Schneider, Schneider S., Shampine L. F., Siebzehnriibl S., Switalski S. C., Teale F. W. J., Wendler J.   +23 more
core   +1 more source

Molecular determinants of signal transduction in tropomyosin receptor kinases

FEBS Open Bio, EarlyView.
Tropomyosin receptor kinases control critical neuronal functions, but how do the same receptors produce diverse cellular responses? This review explores the structural mechanisms behind Trk signaling diversity, focusing on allosteric modulation and ligand bias.
Giray Enkavi
wiley   +1 more source

Controlling plasmon-exciton interactions through photothermal reshaping

Opto-Electronic Advances, 2020
We investigated the plasmon-exciton interactions in an individual gold nanorod (GNR) with monolayer MoS2 at room temperature with the single-particle spectroscopy technique.
Hu Aiqin, Liu Shuai, Zhao Jingyi, Wen Te, Zhang Weidong, Gong Qihuang, Meng Yongqiang, Ye Yu, Lu Guowei   +8 more
doaj   +1 more source