Results 51 to 60 of about 8,507,587 (244)

Laser-induced fluorescence study of OH in flat flames of 1–10 bar compared with resonance CARS experiments [PDF]

, 1990
Kohse-Höinghaus K, Meier U, Attal-Trétout B. Laser-induced fluorescence study of OH in flat flames of 1–10 bar compared with resonance CARS experiments. Applied Optics.
Kohse-Höinghaus, Katharina, Meier, Ulrich, Attal-Trétout, Brigitte   +2 more
core   +1 more source

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

FEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança, Eline L. Jansen, Jorine M. Eeftens   +2 more
wiley   +1 more source

Time-resolved FRET fluorescence spectroscopy of visible fluorescent protein pairs [PDF]

, 2010
Förster resonance energy transfer (FRET) is a powerful method for obtaining information about small-scale lengths between biomacromolecules. Visible fluorescent proteins (VFPs) are widely used as spectrally different FRET pairs, where one VFP acts as a ...
Birch, D.J.S., Laptenok, S., Visser, N.V., Hoek, A., van, Laptenok, S.P., Borst, J.W., Visser, A.J.W.G., van Hoek, A., Brochon, J.C.   +8 more
core   +1 more source

Fluorescence resonance energy transfer sensors for quantitative monitoring of pentose and disaccharide accumulation in bacteria

Biotechnology for Biofuels, 2008
Background Engineering microorganisms to improve metabolite flux requires detailed knowledge of the concentrations and flux rates of metabolites and metabolic intermediates in vivo.
Looger Loren L, Lager Ida, Kaper Thijs, Chermak Diane, Frommer Wolf B   +4 more
doaj   +1 more source

Invisible but not inaccessible—Revealing transient oligomers formed by intrinsically disordered proteins with solution NMR and complementary methods

FEBS Letters, EarlyView.
Transient oligomers formed by intrinsically disordered proteins may be ‘invisible’ to direct detection yet remain accessible to solution NMR through equilibrium‐exchange measurements and pressure‐jump experiments. Complementary methods report on mass, stoichiometry, selected distance distributions, morphology, and internal packing.
Martin D. Gelenter, Ad Bax
wiley   +1 more source

USP29‐regulated noncanonical stabilization of the hypoxia‐inducible factor‐α in aggressive prostate cancer

Molecular Oncology, EarlyView.
We identify USP29 as the only DUB mirroring CA9 expression, a marker of hypoxia and HIF pathway activation associated with PCA aggressiveness. USP29 stabilizes HIF‐1α and HIF‐2α via a noncanonical mechanism that is independent of PHD/pVHL activity yet relies on proteasomal regulation, establishing USP29 as a previously unrecognized regulator of hypoxic
Amelie S Schober, Leire Moreno‐Cugnon, Laura Martínez‐Pérez, Amaia Ercilla, Amaia Zabala‐Letona, Adrián Vicente‐Barrueco, Maider Fagoaga‐Eugui, Saioa Garcia‐Longarte, Blanca Calle‐Ciborro, Encarnación Pérez‐Andrés, Onintza Carlevaris, Sara Pozo, Isabel Mendizabal, Ugo Mayor, Arkaitz Carracedo, Violaine Sée, Edurne Berra   +16 more
wiley   +1 more source

Fluorescence Resonance Energy Transfer Assay for High-Throughput Screening of ADAMTS1 Inhibitors

Molecules, 2011
A disintegrin and metalloprotease with thrombospondin type I motifs-1 (ADAMTS1) plays a crucial role in inflammatory joint diseases and its inhibitors are potential candidates for anti-arthritis drugs.
Guanhua Du, Xiaoyu Bai, Jianhao Peng, Kun Si, Lili Gong   +4 more
doaj   +1 more source

White Light Emission by Simultaneous One Pot Encapsulation of Dyes into One-Dimensional Channelled Aluminophosphate

Nanomaterials, 2020
By simultaneous occlusion of rationally chosen dyes, emitting in the blue, green and red region of the electromagnetic spectrum, into the one-dimensional channels of a magnesium-aluminophosphate with AEL-zeolitic type structure, MgAPO-11, a solid-state ...
Rebeca Sola-Llano, Ainhoa Oliden-Sánchez, Almudena Alfayate, Luis Gómez-Hortigüela, Joaquín Pérez-Pariente, Teresa Arbeloa, Johan Hofkens, Eduard Fron, Virginia Martínez-Martínez   +8 more
doaj   +1 more source

Fluorescence resonance energy transfer between isomers of molecular photoswitches

Nature Communications
Photocontrollable luminescent molecular photoswitches are pivotal for stimuli-responsive smart materials, yet balancing fluorescence with photoswitching performance and enabling isomerization in solid matrices remain challenging.
Yang Xu, Rongpeng Pan, Weike Zou, Yuqi Tang, Quan Li   +4 more
doaj   +1 more source

Simple methods to determine the dissociation constant, Kd

Molecules and Cells
: The determination of the dissociation constant (Kd) is pivotal in biochemistry and pharmacology for understanding binding affinities in chemical reactions, which is crucial for drug development and comprehending biological systems. Here, we introduce a
Donghun Lee, Juwon Kim, Gwangrog Lee
doaj   +1 more source