Results 41 to 50 of about 8,507,587 (244)

FÖRSTER TRANSFER CALCULATIONS BASED ON CRYSTAL STRUCTURE DATA FROM Agmenellum quadruplicatum C-PHYCOCYANIN [PDF]

, 1987
Excitation energy transfer in C-phycocyanin is modeled using the Forster inductive resonance mechanism. Detailed calculations are carried out using coordinates and orientations of the chromophores derived from X-ray crystallographic studies of C ...
Sauer, Kenneth, Scheer, Hugo, Sauer, Peter   +2 more
core   +1 more source

Advanced Data Analysis for Fluorescence-Lifetime Single-Molecule Localization Microscopy

Frontiers in Bioinformatics, 2021
Fluorescence-lifetime single molecule localization microscopy (FL-SMLM) adds the lifetime dimension to the spatial super-resolution provided by SMLM. Independent of intensity and spectrum, this lifetime information can be used, for example, to quantify ...
Jan Christoph Thiele, Oleksii Nevskyi, Dominic A. Helmerich, Markus Sauer, Jörg Enderlein, Jörg Enderlein   +5 more
doaj   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Exploring the structure of the N-terminal domain of CP29 with ultrafast fluorescence spectroscopy [PDF]

, 2009
A high-throughput Förster resonance energy transfer (FRET) study was performed on the approximately 100 amino acids long N-terminal domain of the photosynthetic complex CP29 of higher plants. For this purpose, CP29 was singly mutated along its N-terminal
Koehorst, R.B.M., Rob B. M. Koehorst, van Amerongen, H., Berghuis, B., Laptenok, S., Hoek, A. van, Amerongen, H., van, Berghuis, B.A., Hoek, A., van, Ruud B. Spruijt, Arie van Hoek, van Hoek, A., van Oort, B.F.; id_orcid, Oort, B.F. van, Sergey P. Laptenok, Spruijt, R.B., Bojk A. Berghuis, Bart van Oort, van Oort, B.F., Amerongen, H. van, Oort, B.F., van, Herbert van Amerongen   +21 more
core   +2 more sources

DNA Probes Using Fluorescence Resonance Energy Transfer (FRET): Designs and Applications

BioTechniques, 2001
Fluorescence resonance energy transfer (FRET) is widely used in biomedical research as a reporter method. Oligonucleotides with a DNA backbone and one or several chromophore tags have found multiple applications as FRET probes.
Vladimir V. Didenko
doaj   +1 more source

Modelling stem cell differentiation related processes—A practical overview for biologists

FEBS Letters, EarlyView.
Stem cell differentiation is complex and difficult to control experimentally. This review introduces suitable computational modelling approaches that can support stem cell research, from mechanistic ODE and abstract models to multiscale and deep learning methods.
Ricco Zeegelaar, Xinqi Yan, Lucas F. Jansen Klomp, Stefano Schivo, Janine N. Post   +4 more
wiley   +1 more source

Time-Resolved Fluorescence Investigation of the Human Immunodeficiency Virus Type 1 Nucleocapsid Protein: Influence of the Binding of Nucleic Acids [PDF]

, 1999
Depending on the HIV-1 isolate, MN or BH10, the nucleocapsid protein, NCp7, corresponds to a 55- or 71-amino acid length product, respectively. The MN NCp7 contains a single Trp residue at position 37 in the distal zinc finger motif, and the BH10 NCp7 ...
Piemont, Etienne, Constance Vuilleumier, Etienne Piémont, Gerard, Dominique, Roques, Bernard P., Ababou, Abdessamad, Mely, Yves, Yves Mély, Bombarda, Elisa, Dominique Gérard, Bernard P. Roques, Abdessamad Ababou, Vuilleumier, Constance, Elisa Bombarda   +13 more
core   +1 more source

Synthetic energy sensor AMPfret deciphers adenylate-dependent AMPK activation mechanism

Nature Communications, 2019
AMP-activated protein kinase AMPK senses and regulates cellular energy state. Here the authors engineer a synthetic sensor, AMPfret, that allows direct, real-time readout of the AMPK conformational state by fluorescence resonance energy transfer (FRET).
Martin Pelosse, Cécile Cottet-Rousselle, Cécile M. Bidan, Aurélie Dupont, Kapil Gupta, Imre Berger, Uwe Schlattner   +6 more
doaj   +1 more source

Genotyping of IRGM tetranucleotide promoter oligorepeats by fluorescence resonance energy transfer

BioTechniques, 2009
Fluorescence resonance energy transfer (FRET) genotyping has been well established for the rapid assessment of single nucleotide polymorphisms (SNPs) and deletions. A design is presented that allows the typing of short tandem oligo repeat sequences using
Christopher D. Intemann, Thorsten Thye, Jürgen Sievertsen, Ellis Owusu-Dabo, Rolf D. Horstmann, Christian G. Meyer   +5 more
doaj   +1 more source

Emerging experimental and computational methods for studying redox‐regulated structural transitions

FEBS Letters, EarlyView.
Redox reactions can reshape proteins and alter how they behave in cells, with important consequences for health and disease. This review explores emerging experimental and computational approaches for discovering these redox‐sensitive protein switches, revealing their structural effects, and predicting their behavior, opening new opportunities to ...
Tasneem Rass, Dana Reichmann, Gábor Erdős   +2 more
wiley   +1 more source