Results 51 to 60 of about 204,993 (189)

Discrimination between FRET and non-FRET quenching in a photochromic CdSe quantum dot/dithienylethene dye system [PDF]

, 2014
A photochromic Förster resonance energy transfer (FRET) system was employed to disentangle the fluorescence quenching mechanisms in quantum dot/photochromic dye hybrids.
Dworak, Lars, Reuss, Andreas J., Rück-Braun, Karola, Wachtveitl, Josef, Zastrow, Marc   +4 more
core   +1 more source

Fluorescence resonance energy transfer-based real-time polymerase chain reaction method without DNA extraction for the genotyping of F5, F2, F12, MTHFR, and HFE

Journal of Blood Medicine, 2014
Jordi Martinez-Serra,1 Juan Robles,2 Antoni Nicolàs,3 Antonio Gutierrez,1 Teresa Ros,1 Juan Carlos Amat,1 Regina Alemany,4 Oliver Vögler,4 Aina Abelló,2 Aina Noguera,2 Joan Besalduch1 1Department of Hematology, 2Department of Clinical ...
Martinez-Serra J, Robles J, Nicolàs A, Gutierrez A, Ros T, Amat JC, Alemany R, Vögler O, Abelló A, Noguera A, Besalduch J   +10 more
doaj  

Simple methods to determine the dissociation constant, Kd

Molecules and Cells
: The determination of the dissociation constant (Kd) is pivotal in biochemistry and pharmacology for understanding binding affinities in chemical reactions, which is crucial for drug development and comprehending biological systems. Here, we introduce a
Donghun Lee, Juwon Kim, Gwangrog Lee
doaj   +1 more source

Fluorescence Resonance Energy Transfer Assay for High-Throughput Screening of ADAMTS1 Inhibitors

Molecules, 2011
A disintegrin and metalloprotease with thrombospondin type I motifs-1 (ADAMTS1) plays a crucial role in inflammatory joint diseases and its inhibitors are potential candidates for anti-arthritis drugs.
Guanhua Du, Xiaoyu Bai, Jianhao Peng, Kun Si, Lili Gong   +4 more
doaj   +1 more source

Drug transport mechanism of P-glycoprotein monitored by single molecule fluorescence resonance energy transfer

, 2011
In this work we monitor the catalytic mechanism of P-glycoprotein (Pgp) using single-molecule fluorescence resonance energy transfer (FRET). Pgp, a member of the ATP binding cassette family of transport proteins, is found in the plasma membrane of animal
Boersch, Michael, Ernst, Stefan, Verhalen, Brandy, Wilkens, Stephan, Zarrabi, Nawid   +4 more
core   +1 more source

Steady state fluorescence studies on lipase-vesicle interactions [PDF]

, 1994
The interaction of lipase from Candida cylindracea (CCL) with positively charged polymerizable surfactant vesicles was studied by the use of steady-state fluorescence techniques. The phase transition of vesicles composed of nonpolymerized and polymerized
Engbersen, Johan F.J., Mosmuller, Eduard W.J., Pap, Eward W.H., Visser, Anton J.W.G.   +3 more
core   +3 more sources

Rovibrational Resonance Effects In Collision-Induced Electronic Energy Transfer: I2(E,v=0-2)+CF4 [PDF]

, 2006
Collisions of I-2 in the E(0(g)(+)) electronic state with CF4 molecules induce electronic energy transfer to the nearby D, beta, and D-\u27 ion-pair states. Simulations of dispersed fluorescence spectra reveal collision-induced electronic energy transfer
Carlisle, Benjamin Robert Booth, , \u2705, Hutchison, J. M., Stephenson, Thomas Alex   +2 more
core   +2 more sources

Quantitative Fluorescence Resonance Energy Transfer Measurements Using Fluorescence Microscopy [PDF]

Biophysical Journal, 1998
Fluorescence resonance energy transfer (FRET) is a technique used for quantifying the distance between two molecules conjugated to different fluorophores. By combining optical microscopy with FRET it is possible to obtain quantitative temporal and spatial information about the binding and interaction of proteins, lipids, enzymes, DNA, and RNA in vivo ...
Gordon, Gerald W., Berry, Gail, Liang, Xiao Huan, Levine, Beth, Herman, Brian   +4 more
openaire   +2 more sources

μ-calpain binds to lipid bilayers via the exposed hydrophobic surface of its Ca2+-activated conformation [PDF]

, 2006
μ- and m-calpain are cysteine proteases requiring micro- and millimolar Ca2+ concentrations for their activation in vitro. Among other mechanisms, interaction of calpains with membrane phospholipids has been proposed to facilitate their activation by ...
Assfalg-Machleidt, Irmgard, Fernández-Montalván, Amaury, Fritz, Hans, Jochum, Marianne, Machleidt, Werner, Pfeiler, Dietmar   +5 more
core   +1 more source

fluorescence resonance energy transfer

, 2014
Citation: 'fluorescence resonance energy transfer' in the IUPAC Compendium of Chemical Terminology, 3rd ed.; International Union of Pure and Applied Chemistry; 2006. Online version 3.0.1, 2019. 10.1351/goldbook.FT07378 • License: The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International for individual ...
openaire   +2 more sources