Absence of clustering of phosphatidylinositol-(4,5)-bisphosphate in fluid phosphatidylcholine
Phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2] plays a key role in the modulation of actin polymerization and vesicle trafficking. These processes seem to depend on the enrichment of PI(4,5)P2 in plasma membrane domains. Here, we show that PI(4,5)P2
Faébio Fernandes +3 more
doaj +1 more source
Time-resolved stimulated emission depletion and energy transfer dynamics in two-photon excited EGFP [PDF]
Time and polarization-resolved stimulated emission depletion (STED) measurements are used to investigate excited state evolution following the two-photon excitation of enhanced green fluorescent protein (EGFP).
Bain, AJ
core +1 more source
Optical control of excited-state lifetimes [PDF]
Electronic excitation of particles in fluorescent materials can now be controlled using laser-assisted energy-transfer ...
Andrews, David, Bradshaw, David
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Understanding/unravelling carotenoid excited singlet states. [PDF]
Carotenoids are essential light-harvesting pigments in natural photosynthesis. They absorb in the blue–green region of the solar spectrum and transfer the absorbed energy to (bacterio-)chlorophylls, and thus expand the wavelength range of light that is ...
Alastair T. Gardiner +16 more
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Biophysical detection of diversity and bias in GPCR function
G protein-coupled receptors (GPCRs) function in complexes with a range of molecules and proteins including ligands, G proteins, arrestins, ubiquitin and other receptors.
Werner eJaeger +4 more
doaj +1 more source
Mycobacterium tuberculosis, the causing agent of tuberculosis, comes second only after HIV on the list of infectious agents slaughtering many worldwide. Due to the limitations behind the conventional detection methods, it is therefore critical to develop
Taha Roodbar Shojaei +7 more
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The effects of drug and fatty acid binding on human serum albumin fluorescence [PDF]
In order to create a laboratory experiment focused on Fluorescence Resonance Energy Transfer for undergraduate biochemistry students at Ball State University, Human Serum Albumin, an abundant and sponge-like blood protein, was studied through the eyes
Cale, Eric
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Analysis of Protease Activity Using Quantum Dots and Resonance Energy Transfer
This review demonstrates the detection of protease activity based on the energy transfer of quantum dots (QDs). By incorporation of varying protease substrates into designed QD probes both in fluorescence resonance energy transfer (FRET) and ...
Gae Baik Kim, Young-Pil Kim
doaj
Pulse-shaping-based two-photon FRET microscopy
We present quantitative pulse-shaping-based two-photon fluorescence resonance energy transfer microscopy. We tailor the spectral phase of the excitation pulses to achieve selective excitation of donor and acceptor, demonstrating the method in live cells.
Swanson J. A. +4 more
doaj +1 more source
Interaction of an Antituberculosis Drug with a Nanoscopic Macromolecular Assembly: Temperature-Dependent Förster Resonance Energy Transfer Studies on Rifampicin in an Anionic Sodium Dodecyl Sulfate Micelle [PDF]
In this contribution, we report studies on the nature of binding of a potent antituberculosis drug, Rifampicin (RF) with a model drug delivery system, sodium dodecyl sulfate (SDS) micelle.
Makhal, Abhinandan +3 more
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