Cloning of Dengue Virus Type 2 by the Direct Fluorescent Antibody Technique
SummaryDengue virus type 2 has been cloned in hamster kidney cells, a noncyto-pathic, noncytocidal virus-cell system, utilizing a direct fluorescent antibody method. A clone is considered to have arisen from the infection of a susceptible cell by a single infectious particle of DEN-2.This work was performed under the sponsorship of the Commission on ...
A S, Lubiniecki, G C, Tarr, W M, Hammon
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Childhood pemphigus vulgaris is a challenging diagnosis
Pemphigus Vulgaris (PV) is an uncommon autoimmune and blistering mucocutaneous disease. Childhood Pemphigus Vulgaris (CPV) is a pediatric variant of PV, which affects children below 12 years, being very rare among children under 10 years of age.
Gastão Tenório Lins Filho +6 more
doaj
VIROLOGIC, SEROLOGIC AND PATHOLOGIC STUDIES INTO BORDER DISEASE VIRUS (BDV) INFECTION IN A GOAT HERD IN SAUDIA ARABIA [PDF]
In this study, border disease virus (BDV) infection was suspected in a goat herd in Riyadh province, KSA. The outbreak was unusual in that the typical clinical signs of border disease, i.e., tremor and/or fleece changes were not present; manifestations ...
S.S.A. SHARAWI +3 more
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Direct fluorescent antibody technique for diagnosis of bacterial disease in eel.
Direct fluorescent antibody technique (FAT) has been carried out for diagnosis of Edwardsiella tarda, Aeromonas hydrophila and atypical Aeromonas salmonicida infections. Fluorescent antibody (FA) was prepared against E. tarda EF-l, A. hydrophila CA-1 and atypical A. salmonicida C-5. To remove cross reactivity between A.
Kawahara, Eijiro, Kusuda, Riichi
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Versatile toolbox for high throughput biochemical and functional studies with fluorescent fusion proteins [PDF]
Fluorescent fusion proteins are widely used to study protein localization and interaction dynamics in living cells. However, to fully characterize proteins and to understand their function it is crucial to determine biochemical characteristics such as ...
Sandra B. Hake (165089) +15 more
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Isolation and identification of Bovine Viral Diarrhea virus among cattle and buffaloes in Kalubeya, Egypt (2013-2014) [PDF]
Bovine viral diarrhea virus (BVDV) is the most prevalent infectious disease of cattle. It causes financial losses from avariety of clinical manifestations and is the subject of a number of mitigation and eradication schemes around the world.This study ...
shaimaa Abd-Elhafeez +3 more
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GROWTH STUDIES OF PANLEUKOPENIA VIRUS ON TISSUE CULTURE [PDF]
Feline panleukopenia virus was propagated in different types of cell cultures from different origins and passages: NLFK 224, NLFK319, CRFK, A72, MDCK, Vero and BHK 21. It was found that the most suitable cells for virus propagation were NLFK224.
Attyat M. Kotb +2 more
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ISOLATION AND MOLECULAR IDENTIFICATION OF BHV-1 FROM CATTLE SUFFERING FROM RESPIRATORY SIGNS [PDF]
A total of 93 ocular, 50 nasal swabs and 10 lung tissue were collected from 2-3 years old cattles affected with respiratory manifestation, and 145 serum samples from in contact apparently healthy cattles distributed in 4 different Governorates of Egypt ...
G.H. Salem, Nahed, A. Mahmoud
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Cattle rabies: the effect of clinical evolution, viral genetic lineage, and viral load on the severity of histological lesions [PDF]
: Our objective was the characterization and staging of histological lesions in different anatomical sites of the central nervous system (CNS) of rabid cattle. The severity of the lesions was compared with the clinical stages of the disease, the variants
Claudia S. Wisser +6 more
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Localisation of tissue antigens with the fluorescent antibody technique: studies on connective tissue, the kidney and anterior pituitary hormones [PDF]
Problems involved in the use of the fluorescent antibody technique for the localisation of tissue components are discussed. The need for pure tissue extracts for the immunisation of animals is emphasised and some of the dangers of extraction ...
Cruickshank, Bruce
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