Results 41 to 50 of about 7,369,782 (280)
Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation
FEBS Letters, EarlyView.Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz +11 more
wiley +1 more source
Myxosoma cerebralis: Fluorescent Antibody Techniques for Antigen Recognition
, 1978 Rabbits were immunized with antigens extracted from mature spores or prespore stages of Myxosoma cerebralis, and the resulting antisera and their globulins were used in direct and indirect fluorescent antibody techniques.
Ken Wolf, Maria E. Markiw
core +1 more source
An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes
FEBS Letters, EarlyView.GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg +14 more
wiley +1 more source
Confocal laser scanning microscopy of nanoparticles applied to immunosorbent assays [PDF]
, 2011 The aim of this project was to demonstrate and develop a confocal readout method for fluorescent immunosorbent assays and investigate its potential advantages in comparison to traditional immunoassays.
Ghafari, H
core
Fish Pathology, 1987 1. 直接蛍光抗体法(FAT)を応用して,各種病魚由来のαおよびβ型溶血性の Streptococcus spp.の識別ができるかどうかを検討した。2. その結果,ブリ由来のα 型溶血性株に対する標識抗体はα型菌株のみと,アユ由来のβ型菌株に対する標識抗体はアユ,ブリ,マイワシ由来のβ型溶血性株および S.iniae と特異的に反応した。3. したがって,両者はFATによっても識別できると考えられた。また, S.iniae はβ型溶血性菌株ときわめて類似の抗原構造を有することが示唆された。
KAWAHARA, Eijiro, KUSUDA, Riichi
openaire +2 more sources
Discerning protein pools by selective staining with self‐labeling tags
FEBS Letters, EarlyView.Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley +1 more source
Direct Fluorescent-Antibody Testing Followed by Culture for Diagnosis of 2009 H1N1 Influenza A
, 2011 During the 2009 H1N1 influenza A outbreak, 773 children were tested for influenza by direct fluorescent-antibody testing with PCR confirmation. Direct fluorescent-antibody testing has a specificity of 99.6% but a sensitivity of only 65.0%.
Ora Fried +5 more
core +1 more source
Brazilian Journal of Infectious DiseasesThis study compares the detection rates of Chlamydia trachomatis by two techniques, direct immunofluorescence (IMF) and real time polymerase chain reaction (PCR), in patients with and without intra-epithelial cervical lesions (SIL) in Recife.
Micheline de Lucena Oliveira +5 more
doaj +1 more source
Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics
FEBS Letters, EarlyView.Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt +8 more
wiley +1 more source
DEMONSTRATION OF INTRACELLULAR CARRAGEENAN BY A FLUORESCENT ANTIBODY TECHNIQUE IN GRANULOMA TISSUE
, 1972 Intracellular localization of λ-carrageenan in the carrageenan granuloma has been demonstrated by a specific fluorescent antibody to the polymer. In general, the fluorescent antibody staining correlated with the intracellular metachromatic staining. The
ESTHER L. MCCANDLESS, SUZANNE M. RICHER
core +1 more source