Results 101 to 110 of about 144,270 (311)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

the Use of the Fluorescent Antibody Technique for the Localization of Tissue Antigens.

, 1960
This work was undertaken at the suggestion of Dr. Bram Rose in an attempt to localize reagin in nasal polyps of patients with ragweed hay fever. Though there was much evidence suggesting that reagin must be bound in some way to skin and nasal mucus ...
Leznoff, Arthur.
core  

Studies in epidemiology and seroepidemiology of visceral leishmaniasis in Iraq. [PDF]

A defined population was studied over a period of 7 months to elucidate the epidemiology of visceral leishmaniasis in Iraq, using serological methods as a screening test.
Niazi, Amjad Daoud
core   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Identification of fungi by the fluorescent antibody technique

, 1972
The fluorescent antibody technique was investigated as a means of facilitating the recognition and identification of the fungal components of a western red cedar (Thuja plicata Donn) heartwood flora in situ.
Johnson, Gary Clifford
core  

Emerging Technologies for the Detection of Rabies Virus: Challenges and Hopes in the 21st Century [PDF]

, 2009
The diagnosis of rabies is routinely based on clinical and epidemiological information, especially when exposures are reported in rabies-endemic countries.
Müller, Thomas, Banyard, AC, Conrad M Freuling, Freuling, Conrad Martin, Dastjerdi, A., McElhinney, LM, Dastjerdi, A, Akbar Dastjerdi, Anthony R Fooks, Weiss, RA, Thomas Müller, Freuling, C.M., McElhinney, L.M., Edward Wright, Banyard, A.C., Lorraine M McElhinney, Fooks, AR, Johnson, N., Wright, E, Muller, T, Fooks, A.R., Wakeley, P.R., Wakeley, PR, Robin A Weiss, Philip R Wakeley, Johnson, N, Weiss, R.A., Marston, D.A., Muller, T., Marston, DA, Denise A Marston, Ashley C Banyard, Wright, E., Nicholas Johnson, Freuling, CM   +34 more
core   +1 more source

Combined 5‐aminolevulinic acid and ferric ammonium citrate treatment promotes hair follicle growth by activating dermal papilla cells

FEBS Open Bio, EarlyView.
5‐Aminolevulinic acid combined with ferric ammonium citrate (5‐ALA/FAC) stimulates dermal papilla cell activity and promotes hair follicle growth. The treatment enhances ERK and AKT signaling, increases hair‐inductive gene expression, and restores dermal papilla function suppressed by dihydrotestosterone and oxidative stress, resulting in enhanced hair
Han‐Wook Ryu, Eok‐Soo Oh, Sewoon Kim
wiley   +1 more source

Rabia en Potos flavus identificados en el departamento de madre de dios, Perú

Revista Peruana de Medicina Experimental y Salud Pública
El Potos flavus es un mamífero nocturno que habita en bosques neotropicales desde Centroamérica hasta Sudamérica. Se realizó un estudio de cuatro casos de rabia en Potos flavus ocurridos desde abril de 2012 en el departamento de Madre de Dios en Perú ...
Elena Vargas-Linares, Franco Romaní-Romaní, Ricardo López-Ingunza, Juan Arrasco-Alegre, Martín Yagui-Moscoso   +4 more
doaj  

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Rapid Identification of Streptococcal Infections Using Fluorescent Antibody Techniques

, 1962
The use of fluorescent microscopy coupled with immunological techniques offer new methods for the demonstration of antigen-antibody reactions. The adaptation of these techniques to grouping of beta hemolytic streptococci makes feasible their further ...
Meadows, James H.
core