Results 111 to 120 of about 142,953 (264)

Fluorescent Gonococcal Antibody Technique in Gonorrhea in the Male [PDF]

Public Health Reports (1896-1970), 1963
M Brittain, Moore, E M, Vanderstoep, Reuben D, Wende, John M, Knox   +3 more
openaire   +2 more sources

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Human cryptosporidiosis: detection of specific antibodies in the serum by an indirect immunofluorescence

Revista de Saúde Pública, 1996
Cryptosporidium sp., a coccidian parasite usually found in the faeces of cattle, has been recently implicated as an agent of human intestinal disease, mainly in immunocompromised patients.
Braz Lúcia M.A., Amato Neto Vicente, Ferrari Clara I.L., Palhares Maria C.A., Amato Valdir S., Santos Márcia T.F., Marques Heloísa H.S., Vallada Marcelo, Nakanishi Laura S.S., Andrade Júnior Heitor F.   +9 more
doaj  

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Fluorescent Antibody Technique

The Nishinihon Journal of Dermatology, 1977
MARUTA, Hiroyuki, KAMEYAMA, Akira, INOUE, Kazuhiko   +2 more
openaire   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Study on the value of detecting early secretory antigenic target-6 in cerebrospinal fluid monocytes by laser scanning confocal microscopy techniques in the early diagnosis of tuberculous meningitis

Chinese Journal of Contemporary Neurology and Neurosurgery, 2016
Objective To explore the value of detecting early secretory antigenic target-6 (ESAT-6) in cerebrospinal fluid (CSF) monocytes by laser scanning confocal microscopy (LSCM) techniques in early diagnosis of tuberculous meningitis (TBM), and to find a ...
Mei-jie LI, Yue-li ZOU, Jun-ying HE
doaj  

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Estimation of sensitivity and specificity of antinuclear antibody by automated indirect immunofluorescence and enzyme-linked immunoassay [PDF]

Medical Laboratory Journal
Background: Immunofluorescence and serology analysis are the most common laboratory methods for diagnosing antinuclear antibodies in autoimmune diseases and are paramount for screening and therapeutic purposes. This study aims to estimate the sensitivity
Mohammadreza Sheikh Sajjadieh, Ali Ajami, Leila Haghshenas   +2 more
doaj  

ALDOA Promotes Glycolysis and NLRP3/GSDMD Pyroptosis to Accelerate ALS Progression

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Amyotrophic lateral sclerosis (ALS) is characterized by progressive motor neuron degeneration. Glycolytic dysregulation is implicated in disease progression, yet the underlying mechanisms remain unclear. This study investigates how Aldolase A (ALDOA) drives ALS progression through glycolysis‐mediated motor neuron pyroptosis.
Kaixin Yan, Yan Jiang, Yuxuan Yong, Tianshuo Zhang, Niannian Zhang, Qianqian Zeng, Xue Gong, Li Meng, Fangfang Bi, Yongmin Liu   +9 more
wiley   +1 more source