Results 101 to 110 of about 210,311 (310)

Combination of small molecule microarray and confocal microscopy techniques for live cell staining fluorescent dye discovery [PDF]

open access: yes, 2013
Discovering new fluorochromes is significantly advanced by high-throughput screening (HTS) methods. In the present study a combination of small molecule microarray (SMM) prescreening and confocal laser scanning microscopy (CLSM) was developed in order to
Bokros, Attila   +6 more
core   +1 more source

Acacetin reduces endoplasmic reticulum stress through the P‐eNOS/PERK signaling pathway to attenuate MGO‐induced vascular endothelial cell dysfunction

open access: yesFEBS Open Bio, EarlyView.
We investigated a potential protective effect of acacetin, a naturally occurring flavonoid, against methylglyoxal (MGO)‐induced endothelial dysfunction in human umbilical vein endothelial cells and its mechanism of action. Acacetin can could inhibit MGO‐induced intracellular calcium ion influx, enhance the expression levels of phosphorylated ...
Zhen Zhang   +7 more
wiley   +1 more source

Fluorescent AM1-43 and FM1-43 probes for dental sensory nerves and cells: Their labeling mechanisms and applications

open access: yesJapanese Dental Science Review, 2011
This mini-review first introduces the fluorescent styryl dyes, AM1-43 and FM1-43, which stain a variety of sensory cells and tissues including sensory nerve fibers.
Sumio Nishikawa
doaj   +1 more source

Interchromophoric Interactions Determine the Maximum Brightness Density in DNA Origami Structures [PDF]

open access: yesarXiv, 2019
An ideal point light source is as small and as bright as possible. For fluorescent point light sources, homogeneity of the light sources is important as well as that the fluorescent units inside the light source maintain their photophysical properties which is compromised by dye aggregation.
arxiv  

Size Control and Fluorescence Labeling of Polydopamine Melanin-Mimetic Nanoparticles for Intracellular Imaging. [PDF]

open access: yes, 2017
As synthetic analogs of the natural pigment melanin, polydopamine nanoparticles (NPs) are under active investigation as non-toxic anticancer photothermal agents and as free radical scavenging therapeutics.
Amin, Devang   +3 more
core   +3 more sources

A non‐fluorescent immunohistochemistry method for measuring autophagy flux using MAP1LC3/LC3 and SQSTM1 as core markers

open access: yesFEBS Open Bio, EarlyView.
We introduce an immunohistochemistry method to measure autophagy flux, highlighting the active degradation and recycling of cellular waste. This cost‐effective approach uses tissue samples to track key markers like LC3 and SQSTM1, revealing how cells maintain health or respond to diseases such as cancer. It bridges the gap between research and clinical
Shahla Shojaei   +6 more
wiley   +1 more source

A novel photoacoustic-fluorescent contrast agent for quantitative imaging of lymphatic drainage

open access: yesPhotoacoustics, 2021
In vivo near-infrared (NIR) photoacoustic imaging (PAI) studies using novel contrast agents require validation, often via fluorescence imaging. Bioconjugation of NIR dyes to proteins is a versatile platform to obtain contrast agents for specific ...
Kirsten Cardinell   +7 more
doaj  

Measurement of Temperature Field of a Rayleigh-Benard Convection using Two-Color Laser Induced Fluorescence [PDF]

open access: yes, 2002
Time-dependent instantaneous planar distribution of water temperature in a Rayleigh-Bernard convection was measured by using two-color laser induced fluorescence technique.
Adrian Ronald J.   +2 more
core  

An enzyme‐linked immunosorbent assay (ELISA)‐based activity assay for AMP‐activated protein kinase (AMPK)

open access: yesFEBS Open Bio, EarlyView.
Measuring AMP‐activated protein kinase (AMPK) activity in vitro is crucial for testing AMPK activators or inhibitors with therapeutic potential. Here, we report an enzyme‐linked immunosorbent assay (ELISA)‐based AMPK activity assay with simple steps and high sensitivity, which offers a simple, robust, and cost‐effective alternative to traditional ...
Trezze P. Nguyen, Shangze Lyu, Yang Liu
wiley   +1 more source

Enhanced brightness and photostability of cyanine dyes by supramolecular containment [PDF]

open access: yesarXiv, 2014
Ultrasensitive detection and real-time monitoring of biological processes can benefit significantly from the improved brightness and photostability of the popular organic dyes such as cyanines. Here, using a model cyanine dye, Cy3, we demonstrate that brightness and photostability of the dye is significantly altered when trapped in a molecular ...
arxiv  

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