Results 71 to 80 of about 131,067 (345)

E2A selectively regulates TGF‐β–induced apoptosis in KRAS‐mutant non‐small cell lung cancer

Molecular Oncology, EarlyView.
Ability to induce apoptosis by TGF‐β is frequently lost in advanced lung adenocarcinoma despite intact TGF‐β signaling. We identify E2A as a mutant KRAS–dependent mediator of resistance to TGF‐β–induced apoptosis. TGF‐β induces E2A via SMAD3 in mutant KRAS cells, and E2A silencing restores apoptosis and enhances radiation response in cell lines ...
Sergei Chuikov, Shiva Krishna Katkam, Zhefan Wang, Venkateshwar G. Keshamouni   +3 more
wiley   +1 more source

A simple way to identify non-viable cells within living plant tissue using confocal microscopy

Plant Methods, 2008
Background Plant cell death is a normal process during plant development. Mutant plants may exhibit misregulation of this process, which can lead to severe growth defects. Simple ways of visualising cell death in living plant tissues can aid the study of
Truernit Elisabeth, Haseloff Jim
doaj   +1 more source

Fluorescence efficiency of laser dyes

Journal of Research of the National Bureau of Standards Section A: Physics and Chemistry, 1976
The fluorescence efficiency of xanthene dyes, oxazine dyes, and 7-aminocoumarins is discussed. Relations with the molecular structure are pointed out and dependence on solvent and temperature is explained. Several new fluorescence standards are suggested.
openaire   +1 more source

Heterozygous loss‐of‐function alleles associate the conserved 3′‐5′ exoribonuclease EXOSC10 with hypersensitivity to the anticancer drug 5‐fluorouracil

Molecular Oncology, EarlyView.
EXOSC10, an essential nuclear RNA exosome‐associated 3′‐5′ exoribonuclease, is inhibited by the anticancer drug 5‐fluorouracil (5‐FU), and EXOSC10 depletion increases 5‐FU sensitivity. The colon‐cancer variant EXOSC10S402T, located in a proteolysis motif, is stable and nuclear but nonfunctional in vivo.
Radhika Sain, Yann Le Page, Frédéric Percevault, Emmanuelle Becker, Luc Negroni, Almudena Fernandez, Marta Cantero, Diego Muñoz‐Santos, Lluís Montoliu, Cyrille Garnier, Michael Primig   +10 more
wiley   +1 more source

Wavelength-tuneable liquid crystal lasers from the visible to the near-infrared [PDF]

, 2011
The study of band-edge lasing from dye-doped chiral nematic liquid crystals has thus far been largely restricted to visible wavelengths. In this paper, a wide range of commercially available laser dyes are examined for their suitability as infrared ...
M. M. Qasim, Qasim, M. M., Gardiner, D. J., H. J. Coles, T. D. Wilkinson, Dobson, C. A., Morris, S. M., S. M. Morris, C. A. Dobson, Hands, P. J. W.; id_orcid, D. J. Gardiner, Wilkinson, T. D., P. J. W. Hands, Coles, H. J.   +13 more
core   +1 more source

Photochemistry of Common Xanthene Fluorescent Dyes as Efficient Visible-light Activatable CO-Releasing Molecules

, 2022
Xanthene derivatives are organic dyes, some of which are routinely used in different chemical and biological applications, including human medicine. In this work, we investigated the photochemistry of some of the most common ones, fluorescein, eosin Y ...
Lucie, Muchová, Petr, Klán, Lucie, Ludvíková, Jiří, Huzlík, Mária, Šranková, Peter, Šebej, Rafael, Navrátil, Marek, Martínek, Libor, Vítek   +8 more
core   +1 more source

Clinical performance of the urine‐based TERT promoter AbsoluteQ Digital PCR for non‐invasive detection of bladder cancer

Molecular Oncology, EarlyView.
A urine‐based digital PCR assay targeting two hotspot TERT promoter variants detected bladder cancer with high sensitivity and no false positives in this case–control cohort. The streamlined AbsoluteQ workflow outperformed Sanger sequencing and supports non‐invasive molecular testing for bladder cancer detection.
Anna Nykel, Izabela Kubiak, Lena Rutkowska, Żaneta Kasprzyk, Łukasz Kępczyński, Michał Bednarek, Dariusz Sobieraj, Jacek Wilkosz, Piotr Kania, Adam Jędrzejczyk, Bogdan Kałużewski, Agnieszka Gach, Tadeusz Kałużewski   +12 more
wiley   +1 more source

DiOlistics: Delivery of Fluorescent Dyes into Cells [PDF]

, 2012
DiOlistic labeling utilizes a particle-mediated delivery system to incorporate dye into cells. Because of its random nature, this technique generates sparse fluorescent labeling which is well suited for the study of neuronal dendritic branching and dendritic spine morphology.
Nyssa, Sherazee, Veronica A, Alvarez
openaire   +2 more sources

Application of multiple response optimization design to quantum dot-encoded microsphere bioconjugates hybridization assay [PDF]

, 2011
The optimization of DNA hybridization for genotyping assays is a complex experimental problem that depends on multiple factors such as assay formats, fluorescent probes, target sequence, experimental conditions, and data analysis.
Morgan, Sarah L., Sarah Thiollet, Bessant, Conrad M., Thiollet, Sarah, Conrad Bessant, Sarah L. Morgan   +5 more
core   +1 more source

Imaging of intracellular singlet oxygen with bright BODIPY dyes

, 2021
Singlet oxygen is a cytotoxic reactive species which is involved in the photodynamic therapy of cancer. It is also known to be produced endogenously in most eukaryotic cells and implicated in many biochemical processes, including apoptotic response.
윤주영
core   +1 more source