Results 91 to 100 of about 2,459,362 (303)

Versatile toolbox for high throughput biochemical and functional studies with fluorescent fusion proteins [PDF]

, 2012
Fluorescent fusion proteins are widely used to study protein localization and interaction dynamics in living cells. However, to fully characterize proteins and to understand their function it is crucial to determine biochemical characteristics such as ...
Sandra B. Hake (165089), Antonia Jack (165084), Sandra B Hake, Patricia Wolf, Pichler Garwin, Pichler, Garwin, Hake, Sandra B., Antonia Jack, Wolf Patricia, Garwin Pichler (165082), Jack Antonia, Wolf, Patricia, Jack, Antonia, Hake Sandra B., Garwin Pichler, Patricia Wolf (165088)   +15 more
core   +2 more sources

Computational optical biopsy

BioMedical Engineering OnLine, 2005
Optical molecular imaging is based on fluorescence or bioluminescence, and hindered by photon scattering in the tissue, especially in patient studies. Here we propose a computational optical biopsy (COB) approach to localize and quantify a light source ...
Jiang Ming, Li Yi, Wang Ge
doaj   +1 more source

Partial depletion of plasminogen activator inhibitor‐1 decreases subcutaneous fat cell hypertrophy and liver cholesterol in high‐fat‐fed female mice

FEBS Letters, EarlyView.
Obesity raises blood levels of PAI‐1, a protein linked to metabolic dysfunction‐associated steatotic liver disease in people with obesity. In female mice fed a high‐fat diet, partially lowering PAI‐1 led to smaller subcutaneous fat cells and lower liver cholesterol, without changing body weight or insulin sensitivity.
Claudia E. Ramirez Bustamante, Robert Sharp, Pradip K. Saha, Elizabeth S. Anaya, Elizabeth L. Lieu, Evelyn L. de Groot, Pooja Patel, Mi Jeong Heo, Aaron R. Cox, Kang Ho Kim, Sean M. Hartig   +10 more
wiley   +1 more source

Application and design of esterase-responsive nanoparticles for cancer therapy

Drug Delivery, 2019
Nanoparticles have been developed for tumor treatment due to the enhanced permeability and retention effects. However, lack of specific cancer cells selectivity results in low delivery efficiency and undesired side effects.
Haonan Dong, Long Pang, Hailin Cong, Youqing Shen, Bing Yu   +4 more
doaj   +1 more source

Ligand‐dependent transcriptional heterogeneity in cell cycle gene expression delays G1/S entry

FEBS Letters, EarlyView.
EGF and HRG induce distinct G1/S progression programs in ErbB2‐amplified BT474 breast cancer cells. Despite activating the potent ErbB2–ErbB3 heterodimer, HRG does not accelerate cell‐cycle entry. Instead, EGF promotes earlier restriction‐point passage via ERK–FOS signaling, whereas HRG activates the AKT–MYC axis, driving transcriptional heterogeneity ...
Ririn Rahmala Febri, Ayaka Nagasato‐Ichikawa, Keita Iida, Shuhei Kimura, Akira Imamoto, Mariko Okada   +5 more
wiley   +1 more source

Carbon monoxide‐triggered near‐infrared photoacoustic‐fluorescent integrated visualization tool for auxiliary diagnosis of heart failure and evaluation of reversal drug efficacy

Smart Molecules
Heart failure (HF), a major global health challenge with high morbidity and mortality, lacks effective biomarkers and non‐invasive tools for early diagnosis and therapy evaluation.
Hongze He, Jingrui Yang, Yu Wu, Ping Lin, Tianxiao Lai, Junjie Jia, Jiangfeng Li, Weiying Lin   +7 more
doaj   +1 more source

Emerging experimental and computational methods for studying redox‐regulated structural transitions

FEBS Letters, EarlyView.
Redox reactions can reshape proteins and alter how they behave in cells, with important consequences for health and disease. This review explores emerging experimental and computational approaches for discovering these redox‐sensitive protein switches, revealing their structural effects, and predicting their behavior, opening new opportunities to ...
Tasneem Rass, Dana Reichmann, Gábor Erdős   +2 more
wiley   +1 more source

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

FEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança, Eline L. Jansen, Jorine M. Eeftens   +2 more
wiley   +1 more source

Facile method for CLSM imaging unfunctionalized Au nanoparticles through fluorescent channels

, 2009
The microscopic visualization of metal nanoparticles has become a useful tool for the investigation of their applications in cell labeling and the study of their bio-effects.
Chen, Yueyue, Li, J, Wei, Wei, Sun, Zhiwei, Wang, Hongfang, Zhang, Xiuzhi, Li, Juan, Yuan, Lan   +7 more
core   +1 more source

Simple method for sub-diffraction resolution imaging of cellular structures on standard confocal microscopes by three-photon absorption of quantum dots [PDF]

, 2013
This study describes a simple technique that improves a recently developed 3D sub-diffraction imaging method based on three-photon absorption of commercially available quantum dots.
Heidbreder, Meike, Mike Heilemann, Heilemann, M., Kaltschmidt Christian, Widera, D., Stefan Hauser, Darius Widera (30596), Christian Kaltschmidt, Cseresnyes, Z., Heidbreder Meike, Anje Sporbert, Stefan Hauser (356140), Sporbert Anje, Kaltschmidt, Christian, Kaltschmidt, B., Anje Sporbert (59307), Cseresnyes Zoltan, Heidbreder, M., Kaltschmidt, Barbara, Petra Domaing, Christian Kaltschmidt (30599), Zoltan Cseresnyes (110362), Darius Widera, Hauser Stefan, Kaltschmidt Barbara, Domaing, P., Mike Heilemann (5654950), Sporbert, A., Sporbert, Anje, Widera, Darius, Widera, Darius ; https://orcid.org/, Widera Darius, Barbara Kaltschmidt (30598), Petra Domaing (59308), Meike Heidbreder (414264), Hauser, Stefan, Meike Heidbreder, Barbara Kaltschmidt, Hauser, S., Kaltschmidt, C., Zoltan Cseresnyes, Cseresnyes, Zoltan, Heilemann, Mike, Domaing Petra, Heilemann Mike, Domaing, Petra   +45 more
core   +2 more sources