Results 81 to 90 of about 492,193 (215)

Fluorescent reporters for functional analysis in rice leaves

Plant Direct, 2020
Fluorescent reporters have facilitated non‐invasive imaging in multiple plant species and thus allowed the analysis of processes ranging from gene expression and protein localization to cellular patterning. However, in rice, a globally important crop and
Leonie H. Luginbuehl, Sherif El‐Sharnouby, Na Wang, Julian M. Hibberd   +3 more
doaj   +1 more source

Fluorescence live cell imaging [PDF]

, 2014
Fluorescence microscopy of live cells has become an integral part of modern cell biology. Fluorescent protein (FP) tags, live cell dyes, and other methods to fluorescently label proteins of interest provide a range of tools to investigate virtually any cellular process under the microscope.
Andreas, Ettinger, Torsten, Wittmann
openaire   +2 more sources

Quantitative analysis of chromatin compaction in living cells using FLIM-FRET [PDF]

, 2009
We present a quantitative Forster resonance energy transfer (FRET)-based assay using multiphoton fluorescence lifetime imaging microscopy (FLIM) to measure chromatin compaction at the scale of nucleosomal arrays in live cells. The assay uses a human cell
Angus I. Lamond, Norman, David G., James, John, Lleres, David, Lamond, Angus, I, John James, David Llères, Swift, Sam, David G. Norman, Norman, David, G, Lamond, Angus I.; id_orcid, Sam Swift   +11 more
core   +1 more source

Real-Time analysis and visualization for single-molecule based super-resolution microscopy

, 2013
Accurate multidimensional localization of isolated fluorescent emitters is a time consuming process in single-molecule based super-resolution microscopy.
Jean-Baptiste Sibarita, Kechkar, Adel, Choquet, Daniel, Deepak Nair, Mike Heilemann, Nair, Deepak, Daniel Choquet, Sibarita, Jean-Baptiste, Adel Kechkar, Heilemann, Mike   +9 more
core   +1 more source

Noninvasive molecular imaging of neuroinflammation [PDF]

, 2011
Inflammation is a highly dynamic and complex adaptive process to preserve and restore tissue homeostasis. Originally viewed as an immune-privileged organ, the central nervous system (CNS) is now recognized to have a constant interplay with the innate and
Jacobs, A.H. (Andreas), Bertrand Tavitian, Windhorst, A.D., Lammertsma, A.A., Andreas H Jacobs, Jacobs, A.H., Jacobs, Andreas H., Tavitian, B. (Bertrand), INMiND consortium, Tavitian, Bertrand, Boutin, Herve; id_orcid, Planas, Anna M., Tavitian, B., et al.   +13 more
core   +1 more source

Unveiling Autophagy and Aging Through Time-Resolved Imaging of Lysosomal Polarity with a Delayed Fluorescent Emitter

, 2023
Detecting the lysosomal microenvironmental changes like viscosity, pH, and polarity during their dynamic interorganelle interactions remains an intriguing area that facilitates the elucidation of cellular homeostasis.
Subhadeep, Das, Subhankar , Kundu, Abhilasha , Batra, Abhijit, Patra, Rati, Sharma   +4 more
core   +1 more source

A highly selective turn-on near-infrared fluorescent probe for hydrogen sulfide detection and imaging in living cells

, 2012
We have described a turn-on near-infrared fluorescent probe Cy-NO2 based on nitro group reduction for intracellular H2S detection. The probe employs cyanine dye as a fluorophore, and is equipped with a nitro group as a fluorescent modulator.
Yu, Fabiao, Wang, Linjie, Chen, Lingxin, Zhang, Weiwei, Chen, Hao, Wang, Rui   +5 more
core   +1 more source

Simple method for sub-diffraction resolution imaging of cellular structures on standard confocal microscopes by three-photon absorption of quantum dots

, 2013
This study describes a simple technique that improves a recently developed 3D sub-diffraction imaging method based on three-photon absorption of commercially available quantum dots.
Heidbreder, Meike, Mike Heilemann, Heilemann, M., Kaltschmidt Christian, Widera, D., Stefan Hauser, Darius Widera (30596), Christian Kaltschmidt, Cseresnyes, Z., Heidbreder Meike, Anje Sporbert, Stefan Hauser (356140), Sporbert Anje, Kaltschmidt, Christian, Kaltschmidt, B., Anje Sporbert (59307), Cseresnyes Zoltan, Heidbreder, M., Kaltschmidt, Barbara, Petra Domaing, Christian Kaltschmidt (30599), Zoltan Cseresnyes (110362), Darius Widera, Hauser Stefan, Kaltschmidt Barbara, Domaing, P., Mike Heilemann (5654950), Sporbert, A., Sporbert, Anje, Widera, Darius, Widera, Darius ; https://orcid.org/, Widera Darius, Barbara Kaltschmidt (30598), Petra Domaing (59308), Meike Heidbreder (414264), Hauser, Stefan, Meike Heidbreder, Barbara Kaltschmidt, Hauser, S., Kaltschmidt, C., Zoltan Cseresnyes, Cseresnyes, Zoltan, Heilemann, Mike, Domaing Petra, Heilemann Mike, Domaing, Petra   +45 more
core   +1 more source

Advancing neutron imaging techniques to highest resolution with fluorescent nuclear track detectors

Scientific Reports
Neutron imaging is a nondestructive and noninvasive inspection technique with a wide range of potential applications. However, the fundamentals of this technique still need to be improved, one of which involves achieving micrometer scale or even better ...
Abdul Muneem, Junya Yoshida, Takehiko R. Saito, Hiroyuki Ekawa, Masahiro Hino, Katsuya Hirota, Go Ichikawa, Ayumi Kasagi, Masaaki Kitaguchi, Kenji Mishima, Jameel-Un Nabi, Manami Nakagawa   +11 more
doaj   +1 more source

Fluorescence Lifetime Imaging of Apoptosis

Tomography, 2015
Genetically encoded fluorescence resonance energy transfer (FRET) reporters are powerful tools for analyzing cell signaling and function at single-cell resolution in standard 2D cell cultures, but these reporters rarely have been applied to 3D environments.
Annie Xiao, Anne E. Gibbons, Kathryn E. Luker, Gary D. Luker   +3 more
openaire   +2 more sources