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Digital imaging fluorescence microscopy
1986For digital imaging fluorescence microscopy we have used a Grinnell 274 image processor interfaced to a Lab Datex LSI 11/23 under RT-11 controlled by Integrated Solutions 68010 operating under Unix BSD 4.2. Experimental approaches to address (a) photobleaching of the fluorophore, (b) misalignment of images by nonparfocal microscope optics and (c) the ...
Smith, L.C. +3 more
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Fluorescence lifetime imaging of coral fluorescent proteins
Microscopy Research and Technique, 2007AbstractCorals, like many other coelenterates, contain fluorescent pigments that show considerable homology with the well known green fluorescent protein of the jellyfish Aequoria. In corals, unlike jellyfish, multiple proteins are present and the range of excitations and emissions suggest the possibility of energy transfer.
Guy, Cox, Mikhail, Matz, Anya, Salih
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Image analysis of catecholamine fluorescence
Brain Research Bulletin, 1982Image analysis methods are being developed for measuring the density and distribution of perivascular noradrenergic nerves and their varicosities, demonstrated by fluorescence histochemistry. Image analysis is recommended in comparison with other methods for the quantitation of fluorescence, because image resolution is greater and larger areas of ...
T, Cowen, G, Burnstock
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Fluorescent techniques in thyroid imaging
Seminars in Nuclear Medicine, 1971Fluorescent thyroid imaging is a new technique made possible by the development of the semiconductor detector. Our experience with this instrument in scanning over 159 patients is very encouraging. The primary virtues of the technique are a low radiation dose (only 15 millirad to the gland per scan), no radioactive material injected (no "total body ...
P B, Hoffer, J, Bernstein, A, Gottschalk
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Live‐Cell Fluorescence Imaging
2007This chapter examines the ways to optimize the signal-to-noise ratio while keeping the specimen healthy. Live cells expressing fluorescent protein fusions are usually dim compared to fixed specimens, both because the fluorescent proteins are not very bright and because there is, in most cases, only one fluorophores per protein.
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Image calibration in fluorescence microscopy
Journal of Microscopy, 2004SummaryA fluorescence image calibration method is presented based on the use of standardized uniformly fluorescing reference layers. It is demonstrated to be effective for the correction of non‐uniform imaging characteristics across the image (shading correction) as well as for relating fluorescence intensities between images taken with different ...
Zwier, J.M. +3 more
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Fluorescence lifetime-resolved imaging
Photosynthesis Research, 2009This is a short account of fluorescence lifetime-resolved imaging, in order to acquaint readers who are not experts with the basic methods for measuring lifetime-resolved signals throughout an image. We present the early FLI (fluorescence lifetime imaging) history, review shortly the instrumentation and experimental design, discuss briefly the ...
Yi-Chun, Chen, Robert M, Clegg
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Two-Photon Fluorescence Imaging
2021Two-photon fluorescence imaging is a powerful tool for observing the dynamics of cells in vivo in intact tissue and is well suitable for imaging neuronal activity for neuroscience research. Due to the nonlinear two-photon absorption, the optical sectioning ability is inherent, resulting in two-photon images with high signal contrast and signal-to-noise
Fan, Feng +3 more
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Fluorescence imaging of intracellular Ca2+
Journal of Pharmacological and Toxicological Methods, 1994The measurement of intracellular Ca2+ concentrations ([Ca2+]i) is of critical importance, because many cellular functions are tightly regulated by [Ca2+]i. The fluorescent indicator, fura-2, has been used frequently to measure [Ca2+]i because of its sensitivity and specificity, and because it can be loaded into living cells with little disruption of ...
H, Hayashi, H, Miyata
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Fluorescence Imaging of Membrane Dynamics
Annual Review of Biomedical Engineering, 2008Imaging membrane dynamics is an important goal, motivated by the abundance of biochemical and biophysical events that are orchestrated at, or by, cellular membranes. The short length scales, fast timescales, and environmental requirements of membrane phenomena present challenges to imaging experiments. Several technical advances offer means to overcome
Jay T, Groves +2 more
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