Results 21 to 30 of about 492,193 (215)

Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics [PDF]

, 2013
Genetically encoded calcium indicators (GECIs) are powerful tools for systems neuroscience. Here we describe red, single-wavelength GECIs, RCaMPs, engineered from circular permutation of the thermostable red fluorescent protein mRuby.
Looger, Loren L, Tolö, Johan, Michael B Orger, Leon eLagnado, Tian, Lin, Pulver, Stefan R., Marvin, Jonathan S, Gottschalk, Alexander, Lin eTian, Leon Lagnado, Carreras Calderón, Nicole, Jasper eAkerboom, Kristen E Severi, Eric R Schreiter, Kristen E. Severi, Ronak ePatel, Alexander Gottschalk, Matthias ePrigge, Nicole Carreras Calderón, Kim, Douglas S, Lin Tian, Cornelia I Bargmann, Calderon, Nicole Carreras, Marvin, Jonathan S., Sebastian eKügler, Jasper Akerboom, Douglas S Kim, Douglas S. Kim, Orger, Michael B, Tsai-Wen eChen, Macklin, John J., Sebastian Wabnig, Tsai-Wen Chen, Elisabeth Fischer, Alexander eGottschalk, Elisabeth eFischer, Sarkisyan, Karen S, Marvin, Jonathan, Schreiter, Eric, Matthias Prigge, Wardill, Trevor J, Andrew eGordus, John J Macklin, Schüler, Christina, Trevor J. Wardill, Ronak Patel, Sebastian eWabnig, Severi, Kristen E., Nicole eCarreras Calderón, Severi, Kristen E, Loren L. Looger, Trevor J Wardill, Karen S. Sarkisyan, Christina Schüler, Fischer, Elisabeth, Patel, Ronak, Hegemann, Peter, Stefan R Pulver, Pulver, Stefan R, Kügler, Sebastian, Wardill, Trevor, Eric R. Schreiter, Loren L Looger, Wabnig, Sebastian, Jonathan S. Marvin, Johan Tolö, Sebastian Kügler, Schreiter, Eric R., Patel, Ronak Bhailal, Cornelia I. Bargmann, Sarkisyan, Karen S., Schueler, Christina, John J. Macklin, Andrew Gordus, Karen S Sarkisyan, Chen, Tsai-Wen, Bargmann, Cornelia I., Jonathan S Marvin, Looger, Loren L., Kim, Douglas S., Orger, Michael B., Schreiter, Eric R, Lagnado, Leon, Johan eTolö, Peter Hegemann, Orger, Michael, Prigge, Matthias, Bargmann, Cornelia I, Gordus, Andrew, Christina eSchüler, Stefan R. Pulver, Michael B. Orger, Wardill, Trevor J., Severi, Kristen, Bargmann, Cornelia, Peter eHegemann, Akerboom, Jasper, Macklin, John J   +97 more
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Multicolor lifetime imaging and its application to HIV-1 uptake

Nature Communications, 2023
Simultaneous imaging of nine fluorescent proteins is demonstrated in a single acquisition using fluorescence lifetime imaging microscopy combined with pulsed interleaved excitation of three laser lines.
Tobias Starling, Irene Carlon-Andres, Maro Iliopoulou, Benedikt Kraemer, Maria Loidolt-Krueger, David J. Williamson, Sergi Padilla-Parra   +6 more
doaj   +1 more source

Fluorescent Tracers for In Vivo Imaging of Lymphatic Targets

Frontiers in Pharmacology, 2022
The lymphatic system continues to gain importance in a range of conditions, and therefore, imaging of lymphatic vessels is becoming more widespread for research, diagnosis, and treatment. Fluorescent lymphatic imaging offers advantages over other methods
P. S. Russell, P. S. Russell, R. Velivolu, R. Velivolu, V. E. Maldonado Zimbrón, V. E. Maldonado Zimbrón, J. Hong, J. Hong, J. Hong, I. Kavianinia, I. Kavianinia, A. J. R. Hickey, A. J. R. Hickey, J. A. Windsor, J. A. Windsor, A. R. J. Phillips, A. R. J. Phillips, A. R. J. Phillips   +17 more
doaj   +1 more source

Comparison of the new OPS Imaging technique with intravital microscopy: Analysis of the colon microcirculation [PDF]

, 2002
Background. The OPS imaging technique has been introduced for in vivo assessment of microcirculation in humans. The aim of this study was to validate the new technique against intravital fluorescence microscopy (IFM) for the visualization of colon ...
Langer, S., Biberthaler, Peter
core   +1 more source

Ratiometric fluorescent probe with AIE property for monitoring endogenous hydrogen peroxide in macrophages and cancer cells

Scientific Reports, 2017
Hydrogen peroxide (H2O2) plays a key role in the progression of human illnesses, such as autoimmune and auto-inflammatory diseases, infectious diseases, diabetes, and cancer, etc.
Yong Liu, Jing Nie, Jie Niu, Fangfang Meng, Weiying Lin   +4 more
doaj   +1 more source

ATP changes the fluorescence lifetime of cyan fluorescent protein via an interaction with His148 [PDF]

, 2010
Contains fulltext : 87235.pdf (Publisher’s version ) (Open Access)Recently, we described that ATP induces changes in YFP/CFP fluorescence intensities of Fluorescence Resonance Energy Transfer (FRET) sensors based on CFP-YFP.
Marieke Willemse, Jalink Kees, Fransen, J.A.M., Jalink, K., Sergey P Laptenok, Krogt, G. van der, Krogt, G., Jan Willem Borst, Laptenok Sergey P., Jack A M Fransen, Slijkhuis Rik, Slijkhuis, R., Willemse, M.P., Fransen Jack A. M., Jack A. M. Fransen, Laptenok, S., Willemse, M., Borst, J.W., Wieringa, B., Borst Jan Willem, Gerard van der Krogt, Rik Slijkhuis, Kees Jalink, Sergey P. Laptenok, van der Krogt Gerard, Laptenok, S.P., Be Wieringa, Wieringa Be, Willemse Marieke   +28 more
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Molecular prosthetics for long-term functional imaging with fluorescent reporters

, 2021
Voltage-sensitive fluorescent reporters can reveal fast changes in membrane potential in neurons and cardiomyocytes. However, in many cases, illumination in the presence of the fluorescent reporters results in disruptions to action potential shape that ...
Steven, Boggess, Vincent, Grenier, Samantha, Yang, Brittany, Benlian, Johnathan, Maza, Evan, Miller, Kayli, Martinez, Benjamin, Raliski, Derek, Garcia-Almedina   +8 more
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2-benzothiazoleacetonitrile based two-photon fluorescent probe for hydrazine and its bio-imaging and environmental applications

Scientific Reports, 2017
A novel turn-on two-photon fluorescent probe NS-N 2 H 4 was developed with the 2-benzothiazoleacetonitrile as a new recognition site for the detection of hydrazine (N2H4).
Jian-Yong Wang, Zhan-Rong Liu, Mingguang Ren, Weiying Lin   +3 more
doaj   +1 more source

γPNA FRET Pair Miniprobes for Quantitative Fluorescent In Situ Hybridization to Telomeric DNA in Cells and Tissue

Molecules, 2017
Measurement of telomere length by fluorescent in situ hybridization is widely used for biomedical and epidemiological research, but there has been relatively little development of the technology in the 20 years since it was first reported.
Alexander Orenstein, April S. Berlyoung, Elizabeth E. Rastede, Ha H. Pham, Elise Fouquerel, Connor T. Murphy, Brian J. Leibowitz, Jian Yu, Tumul Srivastava, Bruce A. Armitage, Patricia L. Opresko   +10 more
doaj   +1 more source

Biological nanoscale fluorescent probes: From structure and performance to bioimaging

Reviews in Analytical Chemistry, 2020
In recent years, nanomaterials have attracted lots of attention from researchers due to their unique properties. Nanometer fluorescent materials, such as organic dyes, semiconductor quantum dots (QDs), metal nano-clusters (MNCs), carbon dots (CDs), etc.,
Wan Jiafeng, Zhang Xiaoyuan, Zhang Kai, Su Zhiqiang   +3 more
doaj   +1 more source