Results 31 to 40 of about 2,459,362 (303)

Fluorescent Probes for Nucleic Acid Visualization in Fixed and Live Cells

open access: yesMolecules, 2013
This review analyses the literature concerning non-fluorescent and fluorescent probes for nucleic acid imaging in fixed and living cells from the point of view of their suitability for imaging intracellular native RNA and DNA. Attention is mainly paid to
Alexandre S. Boutorine   +4 more
doaj   +1 more source

Multicolor lifetime imaging and its application to HIV-1 uptake

open access: yesNature Communications, 2023
Simultaneous imaging of nine fluorescent proteins is demonstrated in a single acquisition using fluorescence lifetime imaging microscopy combined with pulsed interleaved excitation of three laser lines.
Tobias Starling   +6 more
doaj   +1 more source

Two-Photon Absorption: An Open Door to the NIR-II Biological Window?

open access: yesFrontiers in Chemistry, 2022
The way in which photons travel through biological tissues and subsequently become scattered or absorbed is a key limitation for traditional optical medical imaging techniques using visible light.
Paige A. Shaw   +5 more
doaj   +1 more source

The Application of Nucleic Acid Probe–Based Fluorescent Sensing and Imaging in Cancer Diagnosis and Therapy

open access: yesFrontiers in Chemistry, 2021
It is well known that cancer incidence and death rates have been growing, but the development of cancer theranostics and therapeutics has been a challenging work. Recently, nucleic acid probe–based fluorescent sensing and imaging have achieved remarkable
Ge Huang   +6 more
doaj   +1 more source

Research progress of near-infrared fluorescence probes based on indole heptamethine cyanine dyes in vivo and in vitro

open access: yesBMC Chemistry, 2020
Near-infrared (NIR) fluorescence imaging is a noninvasive technique that provides numerous advantages for the real-time in vivo monitoring of biological information in living subjects without the use of ionizing radiation. Near-infrared fluorescent (NIRF)
Chunlong Sun   +4 more
doaj   +1 more source

A Versatile Pep-CPDs Nanoprobe for Rapid Detection of mTBI Biomarker in Clinical Instances and Safe Fluorescence Imaging In Vivo for Improved Weight-Drop Mouse Model

open access: yesFrontiers in Bioengineering and Biotechnology, 2022
Mild traumatic brain injury (mTBI) is the most common form of traumatic brain injury; however, it is the most difficult to be accurately identified in the early stage because it lacks more reliable biomarkers and detection methods.
Jian Shi   +6 more
doaj   +1 more source

ATP changes the fluorescence lifetime of cyan fluorescent protein via an interaction with His148 [PDF]

open access: yes, 2010
Contains fulltext : 87235.pdf (Publisher’s version ) (Open Access)Recently, we described that ATP induces changes in YFP/CFP fluorescence intensities of Fluorescence Resonance Energy Transfer (FRET) sensors based on CFP-YFP.
Marieke Willemse   +28 more
core   +3 more sources

γPNA FRET Pair Miniprobes for Quantitative Fluorescent In Situ Hybridization to Telomeric DNA in Cells and Tissue

open access: yesMolecules, 2017
Measurement of telomere length by fluorescent in situ hybridization is widely used for biomedical and epidemiological research, but there has been relatively little development of the technology in the 20 years since it was first reported.
Alexander Orenstein   +10 more
doaj   +1 more source

Green fluorescent protein-based monitoring of endoplasmic reticulum redox poise [PDF]

open access: yes, 2013
Pathological endoplasmic reticulum (ER) stress is tightly linked to the accumulation of reactive oxidants, which can be both upstream and downstream of ER stress.
Julia Birk   +11 more
core   +1 more source

Time-resolved FRET fluorescence spectroscopy of visible fluorescent protein pairs [PDF]

open access: yes, 2010
Förster resonance energy transfer (FRET) is a powerful method for obtaining information about small-scale lengths between biomacromolecules. Visible fluorescent proteins (VFPs) are widely used as spectrally different FRET pairs, where one VFP acts as a ...
Birch, D.J.S.   +8 more
core   +1 more source

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