Results 51 to 60 of about 743,072 (297)

A Versatile, Portable Intravital Microscopy Platform for Studying Beta-cell Biology In Vivo [PDF]

, 2019
The pancreatic islet is a complex micro-organ containing numerous cell types, including endocrine, immune, and endothelial cells. The communication of these systems is lost upon isolation of the islets, and therefore the pathogenesis of diabetes can only
Conteh, Abass M., Day, Richard N., Dunn, Kenneth W., Kamocka, Malgorzata M., Linnemann, Amelia K., Martinez, Michelle M., Mirmira, Raghavendra G., Orr, Kara S., Piñeros, Annie R., Reissaus, Christopher A., Tersey, Sarah A., Twigg, Ashley N.   +11 more
core   +1 more source

Fiber-optic fluorescence imaging [PDF]

Nature Methods, 2005
Optical fibers guide light between separate locations and enable new types of fluorescence imaging. Fiber-optic fluorescence imaging systems include portable handheld microscopes, flexible endoscopes well suited for imaging within hollow tissue cavities and microendoscopes that allow minimally invasive high-resolution imaging deep within tissue.
Benjamin A, Flusberg, Eric D, Cocker, Wibool, Piyawattanametha, Juergen C, Jung, Eunice L M, Cheung, Mark J, Schnitzer   +5 more
openaire   +2 more sources

Simultaneous off-axis multiplexed holography and regular fluorescence microscopy of biological cells

, 2018
We present a new technique for obtaining simultaneous multimodal quantitative phase and fluorescence microscopy of biological cells, providing both quantitative phase imaging and molecular specificity using a single camera.
Barnea, Itay, Nygate, Yoav N., Shaked, Natan T., Singh, Gyanendra   +3 more
core   +1 more source

Millisecond single-molecule localization microscopy combined with convolution analysis and automated image segmentation to determine protein concentrations in complexly structured, functional cells, one cell at a time [PDF]

, 2015
We present a single-molecule tool called the CoPro (Concentration of Proteins) method that uses millisecond imaging with convolution analysis, automated image segmentation and super-resolution localization microscopy to generate robust estimates for ...
Leake, Mark C., Wollman, Adam J. M.
core   +2 more sources

Organoids in pediatric cancer research

FEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

Fluorescence Imaging Study of Film Coating Structure and Composition Effects on DNA Hybridization

Advanced NanoBiomed Research, 2023
Hybridization of surface‐bound DNA with complementary strands is the basis of many biotechnological applications. Herein, the structure of interfacial coatings between substrate and bound DNA is a crucial element for hybridization behavior. Herein, three
Bingquan Yang, Klavdiya Gordiyenko, Andreas Schäfer, Seyed Mohammad Mahdi Dadfar, Wenwu Yang, Kristina Riehemann, Ravi Kumar, Christof M. Niemeyer, Michael Hirtz   +8 more
doaj   +1 more source

Phage-Phenotype Imaging of Myeloma Plasma Cells by Phage Display

Applied Sciences, 2021
Multiple myeloma (MM) is a malignant disease based on differentiated plasma cells (PCs) in the bone marrow (BM). Flow cytometry and fluorescence microscopy, used to identify a large combination of clusters of differentiation (CDs), are applied for MM ...
Laura M. De Plano, Domenico Franco, Martina Bonsignore, Enza Fazio, Sebastiano Trusso, Alessandro Allegra, Caterina Musolino, Riccardo Cavaliere, Guido Ferlazzo, Fortunato Neri, Salvatore P. P. Guglielmino   +10 more
doaj   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Development of a Three-Dimensional Plant Localization Technique for Automatic Differentiation of Soybean from Intra-Row Weeds

Agriculture, 2022
Soybean is a legume that is grown worldwide for its edible bean. Intra-row weeds greatly hinder the normal growth of soybeans. The continuous emergence of herbicide-resistant weeds and the increasing labor costs of weed control are affecting the ...
Wen-Hao Su, Ji Sheng, Qing-Yang Huang
doaj   +1 more source

3D super-resolved in vitro multiphoton microscopy by saturation of excitation

, 2015
We demonstrate a significant resolution enhancement beyond the conventional limit in multiphoton microscopy (MPM) using saturated excitation of fluorescence.
Bouwens, Arno, Duport, François, Egrise, Dominique, Emplit, Philippe, Goldman, Serge, Gorza, Simon-Pierre, Nguyen, Anh Dung, Van Simayes, Gaetan, Vanholsbeeck, Frédérique   +8 more
core   +1 more source