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Fluorescent probes in membrane studies
Philosophical Transactions of the Royal Society of London. B, Biological Sciences, 1972Abstract A number of spectroscopic techniques are suitable for studying biological membranes. Of these, fluorescence has the sensitivity and time resolution for following membrane events associated with nerve excitation. In this paper, the nature of the information derived from measurements of the fluorescence properties of externally
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Fluorescent Probes of Cell Signaling
Annual Review of Neuroscience, 1989Fluorescence has long been recognized as a powerful tool for probing biological structure and function. Because probe molecules can be very much more fluorescent than the constituents of most biological specimens, the signal for the exogenous fluorophores can be measured continuously and nondestructively with excellent spatial and temporal resolution ...
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Free-Probe Fluorescence of Light-up Probes
Journal of the American Chemical Society, 2001The fluorescence enhancement of light-up probes (thiazole orange (TO) conjugated peptide nucleic acids (PNAs)) upon hybridization to target nucleic acid depends on the probe sequence, mainly due to large variations in free-probe fluorescence. Here we study three probes where the fluorescence in free state varies more than 50-fold.
N, Svanvik +3 more
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Novel fluorescent colloids as a DNA fluorescence probe
Analytical and Bioanalytical Chemistry, 2003Fluorescent perylene colloids in the 80-90 nm size range have been prepared by the reprecipitation method. These nanoparticles were modified by cetyltrimethylammonium bromide (CTAB) which inhibited their growth. The nanoparticles also readily interacted with DNA. The fluorescence emission was measured at lambda(ex)/ lambda(em)=400/565 nm.
Liu, Jinshui +4 more
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Fluorescent probes for bioimaging applications
Current Opinion in Chemical Biology, 2008Fluorescent probes based on small organic molecules have become indispensable tools in modern biology because they provide dynamic information concerning the localization and quantity of the molecules of interest, without the need of genetic engineering of the sample.
Takuya, Terai, Tetsuo, Nagano
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Fluorescent probes for opioid receptors
Life Sciences, 1983Naloxone, naltrexone and oxymorphone were labeled in position 6 with fluorescein by coupling their hydrazone analogs with fluorescein isothiocyanate. Naloxone was also coupled in a similar way with rhodamine-B. The compounds thus obtained were: "6-FN", [1-(N)-fluoresceinyl naloxone thiosemicarbazone]; "6-FNX", [1-(N)-fluoresceinyl naltrexone ...
V M, Kolb, A, Koman, L, Terenius
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Fluorescent probes for detecting cysteine
Methods and Applications in Fluorescence, 2018Cysteine plays a crucial role in physiological processes. Therefore, it is necessary to develop a method for detecting cysteine. Fluorimetry has the advantages of convenient detection, short response time, high sensitivity and good selectivity. In this review, fluorescent probes that detect cysteine over the past three years are summarized based on ...
Fanyong Yan +6 more
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Fluorescence Microscopy and Fluorescence Probes
Microscopy and Microanalysis, 1998Fluorescence Microscopy and Fluorescence Probes, edited by Jan Slavik, 1996. Plenum Press, New York and London. 306 pages. (hardback, $95)This volume is a compilation of abstracts of papers presented at the Fluorescence Microscopy and Fluorescence Probes Conference held in Prague in June 1995.
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Fluorescent Probes for Cancer Detection
Journal of Clinical Laser Medicine & Surgery, 1990Several new fluorescent dyes, derivatives of pyrene and of coumarin, were synthesized that have excitation and emission wavelength maxima considerably red-shifted as compared to most pyrene and coumarin compounds. These new fluorescent compounds have high extinction coefficients and high quantum yields, and they also are very environmentally sensitive,
Tong, A., Dowben, R. M., Lin, Tsung-I
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Fluorescent probes for sensing and imaging
Nature Methods, 2011A diverse array of small molecule–based fluorescent probes is available for many different types of biological experiments. Here we examine the history of these probes and discuss some of the most interesting applications.
Tasuku, Ueno, Tetsuo, Nagano
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