Results 51 to 60 of about 274,568 (262)
Journal of Pharmacological Sciences, 2005 Fluorescence resonance energy transfer (FRET) with green fluorescent protein (GFP) variants has become widely used for biochemical research. In order to expand the choice of fluorescent range in FRET analysis, we designed various color versions of the ...
Hiroshi Kawai +12 more
doaj +1 more source
Molecules, 2022 Carbon monoxide (CO) is a vital endogenous gaseous transmitter molecule involved in the regulation of various physiological and pathological processes in living biosystems.
Can Xie +5 more
doaj +1 more source
FEBS Letters, EarlyView.In this study, we found that human cervical‐derived adipocytes maintain intracellular iron level by regulating the expression of iron transport‐related proteins during adrenergic stimulation. Melanotransferrin is predicted to interact with transferrin receptor 1 based on in silico analysis.
Rahaf Alrifai +9 more
wiley +1 more source
Recent Progress in the Rational Design of Biothiol-Responsive Fluorescent Probes
Molecules, 2023 Biothiols such as cysteine, homocysteine, and glutathione play significant roles in important biological activities, and their abnormal concentrations have been found to be closely associated with certain diseases, making their detection a critical task.
Wenzhi Xie +5 more
doaj +1 more source
Tau acetylation at K331 has limited impact on tau pathology in vivo
FEBS Letters, EarlyView.We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto +3 more
wiley +1 more source
Fluorescent Probes of Biological Membranes [PDF]
Proceedings of the National Academy of Sciences, 1970 Fluorescent and phosphorescent probes that have readily interpretable emission properties can be specifically inserted into biological macromolecules to reveal facets of their structure and dynamics: (1) Proximity . Singlet-singlet and triplet-singlet energy transfer can serve as spectroscopic rulers in the 10-65 Å ...
A S, Waggoner, L, Stryer
openaire +2 more sources
Calpain small subunit homodimerization is robust and calcium‐independent
FEBS Letters, EarlyView.Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy +4 more
wiley +1 more source
Sensing Performance and Efficiency of Two Energy Transfer-Based Two-Photon Fluorescent Probes for pH
Sensors, 2018 The design and synthesis of fluorescent probes for monitoring pH values inside living cells have attracted great attention, due to the important role pH plays in many biological processes. In this study, the optical properties of two different two-photon
Yujin Zhang, Wei Hu
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Recent Progress in Fluorescent Probes For Metal Ion Detection
Frontiers in Chemistry, 2022 All forms of life have absolute request for metal elements, because metal elements are instrumental in various fundamental processes. Fluorescent probes have been widely used due to their ease of operation, good selectivity, high spatial and temporal ...
Luanjing Li +4 more
doaj +1 more source
FEBS Letters, EarlyView.Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura +2 more
wiley +1 more source