Results 201 to 210 of about 1,328,987 (298)

MOESM6 of Effect of secretory pathway gene overexpression on secretion of a fluorescent reporter protein in Aspergillus nidulans

, 2016
Martin Schalén, Diana Chinyere Anyaogu, Jakob Blæsbjerg Hoof, Mhairi Workman   +3 more
openalex   +1 more source

Insulin-like Growth Factor Receptor 1 (IGF1R) Gene Copy Number Is Associated With Survival in Operable Non–Small-Cell Lung Cancer: A Comparison Between IGF1R Fluorescent In Situ Hybridization, Protein Expression, and mRNA Expression [PDF]

, 2010
Rafał Dziadziuszko, Daniel T. Merrick, Samir E. Witta, Adelita D. Mendoza, Barbara Szostakiewicz, Amelia Szymanowska, Witold Rzyman, Katarzyna Dziadziuszko, Jacek Jassem, Paul A. Bunn, Marileila Varella‐Garcia, Fred R. Hirsch   +11 more
openalex   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

A fluorescent carbapenem for structure function studies of penicillin-binding proteins, β-lactamases, and β-lactam sensors

, 2014
Cynthia M. June, Robert M. Vaughan, Lucas S. Ulberg, Robert A. Bonomo, Laurie Witucki, David A. Leonard   +5 more
openalex   +1 more source

Ground-state proton transfer in the photoswitching reactions of the fluorescent protein Dronpa [PDF]

, 2013
Mark M. Warren, Marius Kaučikas, Ann Fitzpatrick, P. M. Champion, J. Timothy Sage, Jasper J. van Thor   +5 more
openalex   +1 more source

Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays

FEBS Open Bio, EarlyView.
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul, Alan Strunga, Jiri Damborsky, Zbynek Prokop   +3 more
wiley   +1 more source

Next-generation orange-to-far-red photoconvertible fluorescent protein for single-molecule microscopy and protein dynamic tracking. [PDF]

Sci Rep
Subach OM, Olumayowa F, Tang M, Qiao Y, Zhang Y, Vlaskina AV, Nikolaeva AY, Kononikhin AS, Agapova YK, Belousov A, Borshchevskiy V, Samygina VR, Perfilov MM, Mishin AS, Piatkevich KD, Subach FV.   +15 more
europepmc   +1 more source

Overview of molecular signatures of senescence and associated resources: pros and cons

FEBS Open Bio, EarlyView.
Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas, Sylvia Vagena, Pavlos Pantelis, Giorgos Theocharous, Russel Petty, Konstantinos Evangelou, Vassilis G. Gorgoulis   +6 more
wiley   +1 more source

Tissue-Specific Fluorescent Protein Turnover in Free-Moving Flies. [PDF]

Insects
Bell KS, Ko S, Ali S, Bognar B, Khmelkov M, Rau N, Peng OK, Eyuboglu M, Paine J, Tong A, Saria A, Agrawal S, Davies KJA, Tower J.   +13 more
europepmc   +1 more source

Molecular determinants of signal transduction in tropomyosin receptor kinases

FEBS Open Bio, EarlyView.
Tropomyosin receptor kinases control critical neuronal functions, but how do the same receptors produce diverse cellular responses? This review explores the structural mechanisms behind Trk signaling diversity, focusing on allosteric modulation and ligand bias.
Giray Enkavi
wiley   +1 more source