Results 211 to 220 of about 1,328,987 (298)

Chromophore charge-state switching through copper-dependent homodimerisation of an engineered green fluorescent protein. [PDF]

Chem Sci
Ahmed RD, Vitsupakorn D, Hartwell KD, Albalawi K, Rizkallah PJ, Watson PD, Jones DD.   +6 more
europepmc   +1 more source

Long‐term culture of skin biopsies: maintenance of fibroblast production and competency of reprogramming

FEBS Open Bio, EarlyView.
Skin biopsies taken from a patient with an ultra‐rare disorder as well as controls were cultured for up to 473 days. The chunks of skin were serially transferred to a new culture plate when confluent with fibroblasts. Different generations of fibroblasts were analyzed for cell and molecular properties, proliferation, and competence for reprogramming to
Sudiksha Rathan‐Kumar, Michael A. Ripperger, Grant M. Westlake, Kevin C. Ess   +3 more
wiley   +1 more source

Use of blue fluorescent protein Electra2 for live-cell imaging in <i>Dictyostelium discoideum</i>. [PDF]

MicroPubl Biol
Hashimura H, Nakagawa H, Sawai S.
europepmc   +1 more source

Diabetes‐induced vascular calcification is associated with low pyrophosphate and its oral supplementation prevents calcification in diabetic mice

FEBS Open Bio, EarlyView.
Induction of diabetes in three different mouse strains uniformly resulted in an increase in TNAP activity and a reduction in pyrophosphate (PPi) in the circulation. Inhibition of TNAP restored plasma PPi. Diabetes‐induced calcification in the media layer of the aorta was detected only in the Abcc6−/− strain, which is predisposed to ectopic ...
Krisztina Fülöp, Eszter Kozák, Natália Tőkési, Lilian Kocsis, Anikó Kelemen, Zsuzsanna Geszti, Adriána Kutás, Mariann Harangi, Ágnes Diószegi, Zsolt Rapi, József Balla, Olivier Le Saux, András Váradi, Viola Pomozi   +13 more
wiley   +1 more source

A Set of Fluorescent Protein-Based Markers for Major Vesicle Coat Proteins in Yeast. [PDF]

Membranes (Basel)
Cui XF, Zhang ZT, Zhu J, Cui L, Xie Z.
europepmc   +1 more source

Evaluation of lentiviral vector-based green fluorescent protein expression in human gastric cancer cell line [PDF]

, 2019
Mojtaba Saedi-Marghmaleki, Mohammad-Taghi Moradi, Payam Ghasemi-Dehkordi, Leyla Hashemi, Ali Karimi   +4 more
openalex   +1 more source

The photoswitchable cannabinoid azo‐HU308 enables optical control of Ca2+ dynamics in INS‐1 β‐cells via off‐target effects on TRPC channels

FEBS Open Bio, EarlyView.
Light activation of the photoswitchable cannabinoid ligand azo‐HU308 triggers Ca2+ influx in pancreatic β‐cells through TRPC channels, independent of CB2 cannabinoid receptors. This reveals a non‐GPCR pathway for cannabinoid modulation of β‐cell Ca2+ dynamics and establishes azo‐HU308 as an optical tool to study cannabinoid signaling through TRP ...
Alexander E. G. Viray, James A. Frank
wiley   +1 more source

Ultrafast spectroscopic signatures for <i>off</i>-to-<i>on</i> photoswitchable species in a green-to-red photoconvertible fluorescent protein. [PDF]

Chem Sci
Krueger TD, Chen C, Fang C.
europepmc   +1 more source

Fixation-resistant photoactivatable fluorescent proteins for CLEM

, 2015
Maria G Paez-Segala, Mei Sun, Gleb Shtengel, Sarada Viswanathan, Michelle A. Baird, J. J. Macklin, Ronak Patel, John R. Allen, Elizabeth S. Howe, Grzegorz Piszczek, Harald F. Hess, Michael W. Davidson, Yalin Wang, Loren L. Looger   +13 more
openalex   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source