Results 301 to 310 of about 1,322,177 (348)

Engineering Fluorescent Proteins

2005
Green fluorescent protein from the jellyfish Aequorea victora (GFP) and GFP-like proteins from Anthozoa species encode light-absorbing chromophores intrinsically within their respective protein sequences. Recent studies have made progress in obtaining bright variants of these proteins which develop chromophores quickly and efficiently, as well as novel
Atsushi, Miyawaki, Takeharu, Nagai, Hideaki, Mizuno   +2 more
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GREEN FLUORESCENT PROTEIN

Photochemistry and Photobiology, 1995
Abstract— Several bioluminescent coelenterates use a secondary fluorescent protein, the green fluorescent protein (GFP), in an energy transfer reaction to produce green light. The most studied of these proteins have been the GFPs from the jellyfish Aequorea victoria and the sea pansy Renilla reniformis.
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Optimization of Fluorescent Proteins

2013
Nowadays, fluorescent protein (FP) variants have been engineered to fluoresce in all different colors; to display photoswitchable, or photochromic, behavior; or to show yet other beneficial properties that enable or enhance a still growing set of new fluorescence spectroscopy and microcopy techniques.
Bindels, D.S., Goedhart, J., Hink, M.A., van Weeren, L., Joosen, L., Gadella (jr.), T.W.J.   +5 more
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TYROSINE FLUORESCENCE IN PROTEINS

Annals of the New York Academy of Sciences, 1969
SummaryThe problem of observing tyrosine emission in proteins containing tryptophan is related to both the absorption and emission properties of these two amino acids. First, the absorption of tryptophan is several times larger than tyrosine at all wavelengths.
H, Edelhoch, R L, Perlman, M, Wilchek
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Deconstructing Green Fluorescent Protein

Journal of the American Chemical Society, 2008
Green fluorescent protein (GFP) has been reassembled from two pieces, a large fragment 214 amino acids in length that is produced recombinantly (GFP 1-10) and a short synthetic peptide corresponding to the 11th stave of the beta-barrel that is 16 amino acids long (synthetic GFP 11), following a system developed by Waldo and co-workers (Cabantous, S ...
Kevin P, Kent, William, Childs, Steven G, Boxer   +2 more
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Fluorescent Labeling of Proteins

2011
Many single-molecule experimental techniques exploit fluorescence as a tool to investigate conformational dynamics and molecular interactions or track the movement of proteins in order to gain insight into their biological functions. A prerequisite to these experimental approaches is to graft one or more fluorophores on the protein of interest with the
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Emerging fluorescent protein technologies

Current Opinion in Chemical Biology, 2015
Fluorescent proteins (FPs), such as the Aequorea jellyfish green FP (GFP), are firmly established as fundamental tools that enable a wide variety of biological studies. Specifically, FPs can serve as versatile genetically encoded markers for tracking proteins, organelles, or whole cells, and as the basis for construction of biosensors that can be used ...
Jhon Ralph Enterina, Lanshi Wu, Robert E Campbell   +2 more
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[Orange fluorescent protein--modification of green fluorescent protein GFPxm].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2010
Recently, we have reported a new gfp gene isolated from Aequorea macrodactyla. The protein purified from expressed E. coli exhibited an excitation peak at 476 nm and an emission peak at 496 nm. However, the drawback of only maturing to fluorescence at low temperature limited its applications.
Wen-Xin, Luo, Min, Chen, Tong, Cheng, Bao-Quan, Guan, Shao-Wei, Li, Shao-Jing, Li, Jun, Zhang, Ning-Shao, Xia   +7 more
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The Role of the Protein Matrix in Green Fluorescent Protein Fluorescence

Photochemistry and Photobiology, 2006
ABSTRACTIn the ground state of the highly conjugated green fluorescent protein (GFP), the chromophore should be planar. However, numerous crystal structures of GFP and GFP‐like proteins have been reported with slightly twisted chromophores. We have previously shown that the protein cavity surrounding the chromophore in wild‐type GFP is not ...
Scott L, Maddalo, Marc, Zimmer
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Fluorescent Protein Tracking and Detection: Fluorescent Protein Structure and Color Variants

Cold Spring Harbor Protocols, 2009
INTRODUCTIONThe rapidly growing arsenal of genetically encoded fluorescent proteins (FPs) obtained from sea creatures has launched and fueled a revolution in live cell imaging. The diverse array of applications benefiting from FPs ranges from markers targeted at organelles and protein fusions designed to monitor intracellular dynamics to reporters of ...
Mark A, Rizzo, Michael W, Davidson, David W, Piston   +2 more
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