Results 161 to 170 of about 2,532,057 (298)

Genetically Encoding Stimulated Raman-Scattering Probes for Cell Imaging Using Infrared Fluorescent Proteins. [PDF]

J Phys Chem Lett
Regan D, Aksakal O, McLarnon JJK, Zitti A, Lipka-Lloyd M, Rizkallah PJ, Warren AJ, Watson PD, Langbein W, Jones DD, Borri P.   +10 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Intensity modulation of trichromatic split fluorescent proteins for live cell mapping. [PDF]

Cell Rep Methods
Ishii M, Kinjo T, Kondo Y, Terai K, Aoki K, Kuhlman B, Matsuda M.   +6 more
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Development of far-red fluorescent proteins for temporal domain multiplexing and super-resolution imaging. [PDF]

Cell Rep Methods
Fakorede O, Rong Z, Wang R, Papadaki S, Wang X, Cao J, Subach FV, Köster RW, Namikawa K, Piatkevich KD.   +9 more
europepmc   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Dark-State-Mediated Photobleaching in mCherry-Based Red Fluorescent Proteins. [PDF]

J Phys Chem Lett
Manna P, Hix MA, Mukherjee S, Walker AR, Jimenez R.   +4 more
europepmc   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Intensity-Based Estimation of Monomeric Brightness for Fluorescent Proteins. [PDF]

Int J Mol Sci
Stoneman MR, Bista S, Killeen TD, Popa I, Raicu V.   +4 more
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source