Results 1 to 10 of about 12,307 (185)

Validation and standardization of a fluorometric method for alpha glucosidase activity assay in the detection of Pompe disease [PDF]

open access: yesReviews in Clinical Medicine, 2023
Introduction: Pompe Disease is a type of lysosomal storage disease that is caused by a deficiency of the lysosomal alpha glucosidase. Pompe disease, as a multi-systemic disorder has a broad spectrum of clinical symptoms.
Armin Mokhtariye   +4 more
doaj   +1 more source

Conformational decoupling in acid-sensing ion channels uncovers mechanism and stoichiometry of PcTx1-mediated inhibition

open access: yeseLife, 2022
Acid-sensing ion channels (ASICs) are trimeric proton-gated cation channels involved in fast synaptic transmission. Pharmacological inhibition of ASIC1a reduces neurotoxicity and stroke infarct volumes, with the cysteine knot toxin psalmotoxin-1 (PcTx1 ...
Stephanie A Heusser   +3 more
doaj   +1 more source

Accurate and sensitive probing of onset of micellization based on absolute aggregation‐caused quenching effect

open access: yesAggregate, 2022
Probing the onset of micellization, or determining the critical micelle concentration (CMC), is of crucial importance while remains to be challenged by growing demand for extraordinary sensitivity and accuracy.
Haisheng He   +8 more
doaj   +1 more source

The Activity of NADP(H) Oxidoreductase of Elicitated Cell Culture of Cinchona ledgeriana Moens

open access: yesJurnal Ilmu Pertanian Indonesia, 2020
Cinchona ledgeriana Moens is an industrial plant producing secondary metabolite quinoline alkaloids. To maintain and moreover, to increase the quinoline production especially quinine, in vitro culture system through cell culture could be a potential ...
Maulidiyah Utami   +3 more
doaj   +1 more source

Atmospheric nitrogen deposition and canopy retention influences on photosynthetic performance at two high nitrogen deposition Swiss forests [PDF]

open access: yesTellus: Series B, Chemical and Physical Meteorology, 2012
Portable chlorophyll fluorometry measurements, providing plant photosynthetic efficiency (PE) data, were carried out at two contrasting Swiss forests experiencing high nitrogen (N) deposition. Fluorometry data were obtained in conjunction with controlled
E. Wortman   +7 more
doaj   +1 more source

Recent Advances in Biomolecular Detection Based on Aptamers and Nanoparticles

open access: yesBiosensors, 2023
The fast, accurate detection of biomolecules, ranging from nucleic acids and small molecules to proteins and cellular secretions, plays an essential role in various biomedical applications. These include disease diagnostics and prognostics, environmental
Ruiting Xu   +4 more
doaj   +1 more source

Agarose gel electrophoresis to assess PCR product yield: comparison with spectrophotometry, fluorometry and qPCR

open access: yesBioTechniques, 2022
Agarose gel electrophoresis is a relatively easy to use method, commonly applied to evaluate PCR reaction success. Intercalating agents or dyes are used to visualize the amplified fragments. However, it is uncertain to what extent the brightness of bands
Patrick Wittmeier, Susanne Hummel
doaj   +1 more source

Multifrequency Phase and Modulation Fluorometry [PDF]

open access: yesAnnual Review of Biophysics and Bioengineering, 1984
Ann. Rev. Biophys. Bioeng. 1984. 13: 105-24 MULTIFREQUENCY PHASE AND MODULATION FLUOROMETRY1 Enrico Gratton Department of Physics, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801 David M. Jameson Department of Pharmacology, The University of Texas Health Science Center at Dallas, Dallas, Texas 75235 Robert D.
Gratton, E, Jameson, DM, Hall, RD
openaire   +4 more sources

International Intercomparison of In Situ Chlorophyll-a Measurements for Data Quality Assurance of the Swedish Monitoring Program

open access: yesFrontiers in Remote Sensing, 2022
Chlorophyll-a is an essential climate variable. Chlorophyll-a in situ measurements are usually used for the validation of satellite images. Previous intercomparisons have shown that there can be substantial differences between in situ laboratories.
Susanne Kratzer   +3 more
doaj   +1 more source

Light Stress Responses by the Eelgrass, Zostera marina (L)

open access: yesFrontiers in Environmental Science, 2018
Zostera marina is the dominant seagrass species in the Northern Hemisphere where it grows in sheltered bays and estuaries. As a consequence of its distribution its conservation is commonly threatened by poor coastal water quality.
Chiara M. Bertelli   +1 more
doaj   +1 more source

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