Results 1 to 10 of about 8,111 (160)

Agarose gel electrophoresis to assess PCR product yield: comparison with spectrophotometry, fluorometry and qPCR

BioTechniques, 2022
Agarose gel electrophoresis is a relatively easy to use method, commonly applied to evaluate PCR reaction success. Intercalating agents or dyes are used to visualize the amplified fragments. However, it is uncertain to what extent the brightness of bands
Patrick Wittmeier, Susanne Hummel
doaj   +2 more sources

Comparison of UV spectrometry and fluorometry-based methods for quantification of cell-free DNA in red cell components

Asian Journal of Transfusion Science, 2019
BACKGROUND: Stress and shear force applied on blood components during processing and storage may induce cellular damage leading to release of cell-free DNA (cfDNA).
Dheeraj Khetan, Nitesh Gupta, Rajendra Chaudhary, Jai Shankar Shukla   +3 more
doaj   +2 more sources

High-Sensitivity Fluorometry to Resolve Ion Channel Conformational Dynamics

Cell Reports, 2018
Summary: Fluorescent labels offer the capability to follow conformational dynamics of membrane proteins, but signal detection in such recordings is inherently difficult to achieve in a cell membrane and lacks sufficient time resolution to follow ...
Matthias Wulf, Stephan Alexander Pless
doaj   +2 more sources

Coumarin fluorometry to quantitatively detectable OH radicals in ultrasound aqueous medium

Ultrasonics Sonochemistry, 2016
When ultrasound (US) was exposed to aqueous coumarin solution in air atmosphere, the UV-visible and fluorescence spectra of the probe were measured at different US exposure times.
Takaomi Kobayashi, Kohsuke Hirano
exaly   +2 more sources

Recent developments in the use of differential scanning fluorometry in protein and small molecule discovery and characterization

Expert Opinion on Drug Discovery, 2013
Introduction: Despite tremendous advances in the application of biophysical methods in drug discovery, the preponderance of instruments and techniques still require sophisticated analyses by dedicated personnel and/or large amounts of frequently hard-to ...
Anton Simeonov
exaly   +2 more sources

Validation and standardization of a fluorometric method for alpha glucosidase activity assay in the detection of Pompe disease [PDF]

Reviews in Clinical Medicine, 2023
Introduction: Pompe Disease is a type of lysosomal storage disease that is caused by a deficiency of the lysosomal alpha glucosidase. Pompe disease, as a multi-systemic disorder has a broad spectrum of clinical symptoms.
Armin Mokhtariye, Lida Hagh-Nazari, Abdol-Reza Varasteh, Hadi Mozafari, Fatemeh Keyfi   +4 more
doaj   +1 more source

Toward bio‐optical phenotyping of reef‐forming corals using Light‐Induced Fluorescence Transient‐Fast Repetition Rate fluorometry

Limnology and Oceanography : Methods, 2022
Active chlorophyll a fluorometry is a well‐established tool for noninvasively diagnosing coral functional state, but has not yet been developed as a rapid phenotyping (functional screening) platform as for agriculture and forestry.
D. Suggett, M. Nitschke, D. Hughes, Natasha Bartels, E. Camp, Nicole J. Dilernia, J. Edmondson, S. Fitzgerald, Amanda J. Grima, Ayla Sage, M. Warner   +10 more
semanticscholar   +1 more source

Conformational decoupling in acid-sensing ion channels uncovers mechanism and stoichiometry of PcTx1-mediated inhibition

eLife, 2022
Acid-sensing ion channels (ASICs) are trimeric proton-gated cation channels involved in fast synaptic transmission. Pharmacological inhibition of ASIC1a reduces neurotoxicity and stroke infarct volumes, with the cysteine knot toxin psalmotoxin-1 (PcTx1 ...
Stephanie A Heusser, Christian B Borg, Janne M Colding, Stephan A Pless   +3 more
doaj   +1 more source

Assessing Chlorophyll a Spatiotemporal Patterns Combining In Situ Continuous Fluorometry Measurements and Landsat 8/OLI Data across the Barataria Basin (Louisiana, USA)

Water, 2021
The acquisition of reliable and accurate data to assess environmental changes over large spatial scales is one of the main limitations to determine the impact of eutrophication, and the effectiveness of management strategies in coastal systems.
Ivan Vargas-Lopez, V. H. Rivera-Monroy, J. Day, J. Whitbeck, K. Maiti, C. Madden, A. Trasviña-Castro   +6 more
semanticscholar   +1 more source

Accurate and sensitive probing of onset of micellization based on absolute aggregation‐caused quenching effect

Aggregate, 2022
Probing the onset of micellization, or determining the critical micelle concentration (CMC), is of crucial importance while remains to be challenged by growing demand for extraordinary sensitivity and accuracy.
Haisheng He, Chang Liu, Jiang Ming, Yongjiu Lv, Jianping Qi, Yi Lu, Xiaochun Dong, Weili Zhao, Wei Wu   +8 more
doaj   +1 more source