Results 1 to 10 of about 16,044 (237)

A Low-Cost, Portable, Multi-Cancer Screening Device Based on a Ratio Fluorometry and Signal Correlation Technique [PDF]

open access: yesBiosensors
The autofluorescence of erythrocyte porphyrins has emerged as a potential method for multi-cancer early detection (MCED). With this method’s dependence on research-grade spectrofluorometers, significant improvements in instrumentation are necessary to ...
Abdulaziz S. Alghamdi   +1 more
doaj   +4 more sources

A Four-Wavelength Flow-Through Fluorescence–Scatterometric Sensor That Allows for Real-Time Determination of Fat and Protein Content in Milk–Air Mixtures with High Accuracy [PDF]

open access: yesSensors
(1) Background: Currently, there is a problem of prompt determination of fat and protein content in the milk–air mixture of milking machines.
Maxim E. Astashev   +10 more
doaj   +2 more sources

A simple, reliable and easily generalizable cell-based assay for screening potential drugs that inhibit lipid accumulation [PDF]

open access: yesCurrent Research in Toxicology
Ectopic lipid deposition in the hepatocyte plays an important role in the development of nonalcoholic fatty liver disease (NAFLD), which has become one of the most common causes of chronic liver disease worldwide yet no approved drugs are currently ...
Weili Yang   +4 more
doaj   +2 more sources

Skeletal muscle ribosome analysis: A comparison of common assay methods and utilization of a novel RiboAb antibody cocktail [PDF]

open access: yesPhysiological Reports
While total RNA concentrations putatively represent ribosome content, there is a need to homologize various quantification approaches. Thus, total RNA concentrations ([RNA]) provided through UV–Vis spectroscopy (UV), fluorometry‐only (Fluor), and ...
Joshua S. Godwin   +4 more
doaj   +2 more sources

Chlorophyll Fluorescence in Wheat Breeding for Heat and Drought Tolerance [PDF]

open access: yesPlants
The constantly growing need to increase the production of agricultural products in changing climatic conditions makes it necessary to accelerate the development of new cultivars that meet the modern demands of agronomists. Currently, the breeding process
Firuz Abdullaev   +3 more
doaj   +2 more sources

Validation and standardization of a fluorometric method for alpha glucosidase activity assay in the detection of Pompe disease [PDF]

open access: yesReviews in Clinical Medicine, 2023
Introduction: Pompe Disease is a type of lysosomal storage disease that is caused by a deficiency of the lysosomal alpha glucosidase. Pompe disease, as a multi-systemic disorder has a broad spectrum of clinical symptoms.
Armin Mokhtariye   +4 more
doaj   +1 more source

Conformational decoupling in acid-sensing ion channels uncovers mechanism and stoichiometry of PcTx1-mediated inhibition

open access: yeseLife, 2022
Acid-sensing ion channels (ASICs) are trimeric proton-gated cation channels involved in fast synaptic transmission. Pharmacological inhibition of ASIC1a reduces neurotoxicity and stroke infarct volumes, with the cysteine knot toxin psalmotoxin-1 (PcTx1 ...
Stephanie A Heusser   +3 more
doaj   +1 more source

Accurate and sensitive probing of onset of micellization based on absolute aggregation‐caused quenching effect

open access: yesAggregate, 2022
Probing the onset of micellization, or determining the critical micelle concentration (CMC), is of crucial importance while remains to be challenged by growing demand for extraordinary sensitivity and accuracy.
Haisheng He   +8 more
doaj   +1 more source

Agarose gel electrophoresis to assess PCR product yield: comparison with spectrophotometry, fluorometry and qPCR

open access: yesBioTechniques, 2022
Agarose gel electrophoresis is a relatively easy to use method, commonly applied to evaluate PCR reaction success. Intercalating agents or dyes are used to visualize the amplified fragments. However, it is uncertain to what extent the brightness of bands
Patrick Wittmeier, Susanne Hummel
doaj   +1 more source

The Activity of NADP(H) Oxidoreductase of Elicitated Cell Culture of Cinchona ledgeriana Moens

open access: yesJurnal Ilmu Pertanian Indonesia, 2020
Cinchona ledgeriana Moens is an industrial plant producing secondary metabolite quinoline alkaloids. To maintain and moreover, to increase the quinoline production especially quinine, in vitro culture system through cell culture could be a potential ...
Maulidiyah Utami   +3 more
doaj   +1 more source

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