Results 161 to 170 of about 17,962 (220)

Laboratory Algal Bioassays Using PAM Fluorometry: Effects of Test Conditions on the Determination of Herbicide and Field Sample Toxicity

open access: yesEnvironmental Toxicology and Chemistry, 2014
Pulse Amplitude Modulation (PAM) fluorometry, based on chlorophyll a fluorescence, is a frequently used technique in algal bioassays to assess toxicity of single compounds or complex field samples.
Wim Admiraal   +2 more
exaly   +2 more sources

Sensitivity Evaluation of the Green Alga Chlamydomonas reinhardtii to Uranium by Pulse Amplitude Modulated (PAM) Fluorometry

open access: yesAquatic Toxicology, 2013
International audienceAlthough ecotoxicological studies tend to address the toxicity thresholds of uranium in freshwaters, there is a lack of information on the effects of the metal on physiological processes, particularly in aquatic plants. Knowing that
Rodolphe Gilbin, Jean-Marc Bonzom
exaly   +2 more sources
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Clinical applications of fluorometry

Documenta Ophthalmologica, 1983
An instrument has been developed that measures in ten seconds the level of fluorescein along a scanning line from the retina to the cornea. With spatial resolving power of 1.1 mm in the eye, concentrations of 1 X 10(-8) gr/ml fluorescein and higher are detectable. In this way an indication of the integrity of the blood-ocular barriers is achieved.
Blanksma, L.J.   +3 more
openaire   +3 more sources

Microcapillary Fluorometry and Standardization for Microscope Fluorometry

1973
Standardization in fluorometry is still connected with difficulties. The theoretical and practical reasons for this have recently been discussed during the National Bureau of Standards conference in Washington (Conference on Accuracy). In the field of microscope fluorometry standardization is even more difficult because of the tiny volumes and amounts ...
M. Sernetz, A. Thaer
openaire   +1 more source

Filterless Fluorometry with Enhanced Sensitivity

Journal of Fluorescence, 2008
We describe a novel approach for fluorometry with light-emitting-diodes (LEDs) as excitation source that capitalizes on the principle of light shielding and total internal reflection to operate without optical filters. This scheme is practicable, demonstrated to significantly reduce the amount of excitation light overlapping the fluorescence signals at
Han Yen, Tan, Tuck Wah, Ng, Oi Wah, Liew
openaire   +2 more sources

DNA-fluorometry of mammalian sperm

Histochemistry, 1977
The Dna-content of sperm and testicular cells was measured by pulse-cytophotometry with high resolution. From flat sperm symmetric and narrow fluorescene distributions were obtained. Enzymatic treatment with papain or pronase and staining with an ethidiumbromide-mithramycin dye solution generate stoichiometric DNA-staining including that of mature ...
J, Zante   +3 more
openaire   +2 more sources

Sensitive Detection of Genes by Sandwich Hybridization and Time-Resolved Fluorometry

open access: yesMolecular and Cellular Probes, 1987
Europium has been used as a non-radioactive marker in immunoassays as this metal can be detected with high sensitivity by time-resolved fluorometry. In this work streptavidin labeled with europium was used to detect biotinylated probes in a sandwich ...
Ann-Christine Syvänen   +2 more
exaly   +2 more sources

Application of fluorometry in the determination of Vitamin A

Analytical Biochemistry, 1968
Abstract A micromethod is described for the determination of Vitamin A in blood and tissues, based upon application of fluorometric and thin-layer chromatographic techniques. The method is sensitive and relatively rapid. The vitamin can be quantitatively estimated in a blood sample of 0.1 ml, obtained from a finger-tip puncture or in a tissue sample
B D, Drujan, R, Castillon, E, Guerrero
openaire   +2 more sources

The Voltage-Clamp Fluorometry Technique

2008
Ion channels are the cell's gatekeepers. These proteins selectively allow ionic current to flow down its electrochemical gradient. In some cases, specialized chemical or voltage sensing domains respond to environmental changes and signal the cell to adjust its internal chemistry in response to its surroundings.
Chris S, Gandhi, Riccardo, Olcese
openaire   +2 more sources

Functional Site-Directed Fluorometry

2015
Initially developed in the mid-1990s to examine the conformational changes of the canonical Shaker voltage-gated potassium channel, functional site-directed fluorometry has since been expanded to numerous other voltage-gated and ligand-gated ion channels as well as transporters, pumps, and other integral membrane proteins.
Michael, Priest, Francisco, Bezanilla
openaire   +2 more sources

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