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Pulse Amplitude Modulation (PAM) fluorometry, based on chlorophyll a fluorescence, is a frequently used technique in algal bioassays to assess toxicity of single compounds or complex field samples.
Wim Admiraal +2 more
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International audienceAlthough ecotoxicological studies tend to address the toxicity thresholds of uranium in freshwaters, there is a lack of information on the effects of the metal on physiological processes, particularly in aquatic plants. Knowing that
Rodolphe Gilbin, Jean-Marc Bonzom
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Clinical applications of fluorometry
Documenta Ophthalmologica, 1983An instrument has been developed that measures in ten seconds the level of fluorescein along a scanning line from the retina to the cornea. With spatial resolving power of 1.1 mm in the eye, concentrations of 1 X 10(-8) gr/ml fluorescein and higher are detectable. In this way an indication of the integrity of the blood-ocular barriers is achieved.
Blanksma, L.J. +3 more
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Microcapillary Fluorometry and Standardization for Microscope Fluorometry
1973Standardization in fluorometry is still connected with difficulties. The theoretical and practical reasons for this have recently been discussed during the National Bureau of Standards conference in Washington (Conference on Accuracy). In the field of microscope fluorometry standardization is even more difficult because of the tiny volumes and amounts ...
M. Sernetz, A. Thaer
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Filterless Fluorometry with Enhanced Sensitivity
Journal of Fluorescence, 2008We describe a novel approach for fluorometry with light-emitting-diodes (LEDs) as excitation source that capitalizes on the principle of light shielding and total internal reflection to operate without optical filters. This scheme is practicable, demonstrated to significantly reduce the amount of excitation light overlapping the fluorescence signals at
Han Yen, Tan, Tuck Wah, Ng, Oi Wah, Liew
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DNA-fluorometry of mammalian sperm
Histochemistry, 1977The Dna-content of sperm and testicular cells was measured by pulse-cytophotometry with high resolution. From flat sperm symmetric and narrow fluorescene distributions were obtained. Enzymatic treatment with papain or pronase and staining with an ethidiumbromide-mithramycin dye solution generate stoichiometric DNA-staining including that of mature ...
J, Zante +3 more
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Sensitive Detection of Genes by Sandwich Hybridization and Time-Resolved Fluorometry
Europium has been used as a non-radioactive marker in immunoassays as this metal can be detected with high sensitivity by time-resolved fluorometry. In this work streptavidin labeled with europium was used to detect biotinylated probes in a sandwich ...
Ann-Christine Syvänen +2 more
exaly +2 more sources
Application of fluorometry in the determination of Vitamin A
Analytical Biochemistry, 1968Abstract A micromethod is described for the determination of Vitamin A in blood and tissues, based upon application of fluorometric and thin-layer chromatographic techniques. The method is sensitive and relatively rapid. The vitamin can be quantitatively estimated in a blood sample of 0.1 ml, obtained from a finger-tip puncture or in a tissue sample
B D, Drujan, R, Castillon, E, Guerrero
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The Voltage-Clamp Fluorometry Technique
2008Ion channels are the cell's gatekeepers. These proteins selectively allow ionic current to flow down its electrochemical gradient. In some cases, specialized chemical or voltage sensing domains respond to environmental changes and signal the cell to adjust its internal chemistry in response to its surroundings.
Chris S, Gandhi, Riccardo, Olcese
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Functional Site-Directed Fluorometry
2015Initially developed in the mid-1990s to examine the conformational changes of the canonical Shaker voltage-gated potassium channel, functional site-directed fluorometry has since been expanded to numerous other voltage-gated and ligand-gated ion channels as well as transporters, pumps, and other integral membrane proteins.
Michael, Priest, Francisco, Bezanilla
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