Results 181 to 190 of about 16,044 (237)
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Filterless Fluorometry with Enhanced Sensitivity
Journal of Fluorescence, 2008We describe a novel approach for fluorometry with light-emitting-diodes (LEDs) as excitation source that capitalizes on the principle of light shielding and total internal reflection to operate without optical filters. This scheme is practicable, demonstrated to significantly reduce the amount of excitation light overlapping the fluorescence signals at
Han Yen, Tan, Tuck Wah, Ng, Oi Wah, Liew
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DNA-fluorometry of mammalian sperm
Histochemistry, 1977The Dna-content of sperm and testicular cells was measured by pulse-cytophotometry with high resolution. From flat sperm symmetric and narrow fluorescene distributions were obtained. Enzymatic treatment with papain or pronase and staining with an ethidiumbromide-mithramycin dye solution generate stoichiometric DNA-staining including that of mature ...
J, Zante +3 more
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EVALUATION OF THE BURN WOUND WITH PERFUSION FLUOROMETRY
The Journal of Trauma: Injury, Infection, and Critical Care, 1982Determination of depth of burn injury using vital dyes has been unsatisfactory. The present study evaluated the ability of the fiberoptic perfusion fluorometer to assess the depth of burn in the early postburn period. Sixty-three burns were examined with the fluorometer after intravenous administration of sodium fluorescein.
J E, Gatti +3 more
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The Voltage-Clamp Fluorometry Technique
2008Ion channels are the cell's gatekeepers. These proteins selectively allow ionic current to flow down its electrochemical gradient. In some cases, specialized chemical or voltage sensing domains respond to environmental changes and signal the cell to adjust its internal chemistry in response to its surroundings.
Chris S, Gandhi, Riccardo, Olcese
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Functional Site-Directed Fluorometry
2015Initially developed in the mid-1990s to examine the conformational changes of the canonical Shaker voltage-gated potassium channel, functional site-directed fluorometry has since been expanded to numerous other voltage-gated and ligand-gated ion channels as well as transporters, pumps, and other integral membrane proteins.
Michael, Priest, Francisco, Bezanilla
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Spectrophotometry and Fluorometry
1981Before going into detail, it may be advisable to provide an overview of the principles that underlie the techniques to be discussed in this chapter. The most important physical principles involve the relationships between the wave and particle properties of both light and matter and the interaction of light with matter.
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Journal of Physics E: Scientific Instruments, 1988
The authors demonstrate the simultaneous measurement of fluorescence decays at different emission wavelengths using single-photon timing array detection.
D J S Birch +4 more
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The authors demonstrate the simultaneous measurement of fluorescence decays at different emission wavelengths using single-photon timing array detection.
D J S Birch +4 more
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Fluorometry and Phosphorimetry in Clinical Chemistry
1970Publisher Summary Fluorometry and phosphorimetry are analytical techniques now widely used in clinical chemistry. Fluorometry depends upon the generation of light energy by reactions involving the analytical sample. Analysis by phosphorescence, although less popular, has some unusual characteristics, which lead to the expectation that it can find a ...
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Oftalmologia (Bucharest, Romania : 1990), 2007
Vitreous fluorophotometry is an investigation that facilitates the quantitative study of the blood-retinal barrier (thus allowing a better understanding of its physiopathology and opening new therapeutical research fields). It can be performed for the vast majority of the ocular diseases: diabetic retinopathy, arterial hypertension, uveitis, post ...
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Vitreous fluorophotometry is an investigation that facilitates the quantitative study of the blood-retinal barrier (thus allowing a better understanding of its physiopathology and opening new therapeutical research fields). It can be performed for the vast majority of the ocular diseases: diabetic retinopathy, arterial hypertension, uveitis, post ...
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Fluorometry of the Anterior Segment
1990The anterior segment includes four transparent tissues and fluids—tear film, cornea, aqueous humor, and lens—that can be directly illuminated and observed by optical systems distant from the eye. It forms a system well adapted to the measurement of fluorescence in a noninvasive manner.
Richard F. Brubaker +2 more
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