Results 181 to 190 of about 27,523 (221)

Functional Site-Directed Fluorometry

2015
Initially developed in the mid-1990s to examine the conformational changes of the canonical Shaker voltage-gated potassium channel, functional site-directed fluorometry has since been expanded to numerous other voltage-gated and ligand-gated ion channels as well as transporters, pumps, and other integral membrane proteins.
Michael, Priest, Francisco, Bezanilla
openaire   +2 more sources

Filterless Fluorometry with Enhanced Sensitivity

Journal of Fluorescence, 2008
We describe a novel approach for fluorometry with light-emitting-diodes (LEDs) as excitation source that capitalizes on the principle of light shielding and total internal reflection to operate without optical filters. This scheme is practicable, demonstrated to significantly reduce the amount of excitation light overlapping the fluorescence signals at
Han Yen, Tan, Tuck Wah, Ng, Oi Wah, Liew
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Microcapillary Fluorometry and Standardization for Microscope Fluorometry

1973
Standardization in fluorometry is still connected with difficulties. The theoretical and practical reasons for this have recently been discussed during the National Bureau of Standards conference in Washington (Conference on Accuracy). In the field of microscope fluorometry standardization is even more difficult because of the tiny volumes and amounts ...
M. Sernetz, A. Thaer
openaire   +1 more source

Library Quantification Using PicoGreen Fluorometry

Cold Spring Harbor Protocols, 2016
This protocol describes the quantification of DNA using PicoGreen and a fluorometer to determine the concentration of DNA sample for downstream processing. Because dye-based methods will not detect degraded or short DNA fragments, PicoGreen requires DNAs ≥50 bp, but can less reliably detect fragments as small as 20 bp.
Elaine, Mardis, W Richard, McCombie
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Front-face fluorometry of liquid samples

Analytical Biochemistry, 1979
Abstract Quantitative analytical spectrofluorometry is usually performed with solutions which are optically dilute at the excitation wavelength and employs square cuvettes for observation at right angles to the excitation beam. In many biochemical applications, particularly when studying fluorophores in blood, front-face fluorometry of optically ...
J, Eisinger, J, Flores
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The Voltage-Clamp Fluorometry Technique

2008
Ion channels are the cell's gatekeepers. These proteins selectively allow ionic current to flow down its electrochemical gradient. In some cases, specialized chemical or voltage sensing domains respond to environmental changes and signal the cell to adjust its internal chemistry in response to its surroundings.
Chris S, Gandhi, Riccardo, Olcese
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[Vitreal fluorometry].

Oftalmologia (Bucharest, Romania : 1990), 2007
Vitreous fluorophotometry is an investigation that facilitates the quantitative study of the blood-retinal barrier (thus allowing a better understanding of its physiopathology and opening new therapeutical research fields). It can be performed for the vast majority of the ocular diseases: diabetic retinopathy, arterial hypertension, uveitis, post ...
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Visible semiconductor laser fluorometry

Analytical Chemistry, 1989
Un laser oscillant a 670 nm est utilise comme source d'excitation en spectrometrie de fluorescence. Une limite de detection de 4×10 −12 M a ete obtenue pour la rhodamine 800, legerement meilleure que celle obtenue avec un laser semiconducteur proche ...
Totaro. Imasaka, Atsushi. Tsukamoto, Nobuhiko. Ishibashi   +2 more
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Detector nonlinearity in frequency-domain fluorometry

Applied Optics, 1993
Frequency-domain fluorometry relies on the measurement of the phase and amplitudes of the Fourier components of the time-dependent fluorescence signal. Experimental results that show that a conventional photomultiplier is subject to intensity-dependent phase shifts are presented.
M J, Wirth, J D, Burbage, S L, Zulli
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Stopped-flow fluorometry with available instrumentation

Analytical Biochemistry, 1969
Abstract Stopped-flow fluorescence experiments were conducted on a microscale in a spectrofluorometer by using an air-driven mixer attachment. All parts of the system employed, including the electronic components, are commercially available. There are many biochemical reactions of interest which take place in 0.05 to 10 seconds, that can be studied ...
R F, Chen, A N, Schechter, R L, Berger
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