Results 131 to 140 of about 1,609,713 (317)

Geotechnology as a Planning Tool in the Determination of Forest Extraction Costs

open access: yesFloresta e Ambiente
The objective of this work was to use the geotechnology study as a planning tool in determining the costs of forest extraction. The research was carried out in areas of the Forest Company that operates in the southern region of Bahia, Brazil.
Francisco de Assis Costa Ferreira   +3 more
doaj   +1 more source

HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein

open access: yesFEBS Open Bio, EarlyView.
Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

open access: yesFEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn   +4 more
wiley   +1 more source

PARP inhibitors induce a senescence phenotype in non‐small cell lung carcinoma cell lines

open access: yesFEBS Open Bio, EarlyView.
Talazoparib is the most potent inducer of senescence among different PARP1 inhibitors in human NSCLC cells. In the absence of PARP, no senescence phenotype was observed, demonstrating that PARP1 is necessary for the induction of senescence by this inhibitor.
Camille Huart   +7 more
wiley   +1 more source

Mathematical Modeling of Forwarder Operation When Collecting Logs in the Forest Swath

open access: yesTehnički Glasnik
Optimization of the machines allows you to increase their productivity and reduce the labor costs for performing individual elements of the technological cycle of their work in the forest.
Konstantin P. Rukomojnikov   +2 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Effects of Forwarder Operation on Soil Physical Characteristics: a Case Study in the Italian Alps

open access: yesCroatian Journal of Forest Engineering, 2016
One of the most important issues in ground based wood extraction in forestry is to minimize the soil damage caused by heavy forestry machines. Generally, harvesting effects include changes in vegetation nutrient availability, soil microclimate/structure ...
Stefano Grigolato   +4 more
doaj  

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