Purification of Starch Granules from Arabidopsis Leaves and Determination of Granule-Bound Starch Synthase Activity [PDF]
Bio-Protocol, 2014 Starch constitutes the most important carbon reserve in plants and is composed of branched amylopectin and linear amylose. The latter is synthesized exclusively by the Granule-Bound Starch Synthase (GBSS, EC 2.4.1.21).
Tomás Albi +4 more
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Plastid 2-Cys peroxiredoxins are essential for embryogenesis in Arabidopsis [PDF]
Redox Biology, 2023 The redox couple formed by NADPH-dependent thioredoxin reductase C (NTRC) and 2-Cys peroxiredoxins (Prxs) allows fine-tuning chloroplast performance in response to light intensity changes.
Antonia M. Gallardo-Martínez +4 more
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The contribution of glutathione peroxidases to chloroplast redox homeostasis in Arabidopsis [PDF]
Redox Biology, 2023 Oxidizing signals mediated by the thiol-dependent peroxidase activity of 2-Cys peroxiredoxins (PRXs) plays an essential role in fine-tuning chloroplast redox balance in response to changes in light intensity, a function that depends on NADPH-dependent ...
Azahara Casatejada +3 more
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Enhanced sucrose production by controlling carbon flux through CfrA expression in Synechocystis sp. PCC 6803 [PDF]
Microbial Cell FactoriesBackground Cyanobacteria, as phototrophic organisms with low nutritional requirements and great metabolic versatility, are attractive for the sustainable production of value-added chemicals from CO2 and sunlight.
María Teresa Domínguez-Lobo +3 more
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Isolation of Ribosomal Particles from the Unicellular Cyanobacterium Synechocystis sp. PCC 6803 [PDF]
Bio-Protocol, 2017 Isolation of ribosomal particles is an essential step in the study of ribosomal components as well as in the analysis of trans-acting factors that interact with the ribosome to regulate protein synthesis and modulate the expression profile of the cell in
Carla Galmozzi, M. Isabel Muro-Pastor
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Determination of Soluble Sugars in Arabidopsis thaliana Leaves by Anion Exchange Chromatography [PDF]
Bio-Protocol, 2014 Determination of soluble sugars is basic for the study of carbon metabolism in plants. Soluble sugar quantitation can be achieved by enzymatic methods implying different coupled reactions.
M. Ortiz-Marchena +3 more
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Combined Effect of Temperature and Different Light Regimes on the Photosynthetic Activity and Lipid Accumulation in the Diatom Phaeodactylum tricornutum [PDF]
PlantsThe aim of this study was to investigate the combined effects of temperature and light on the photosynthetic parameters and lipid accumulation in the diatom Phaeodactylum tricornutum, a model organism widely used for studies on diatom physiology, ecology,
Encarnación Díaz-Santos +6 more
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Intercellular communication in the fern endosymbiotic cyanobacterium Nostoc azollae [PDF]
mBioThe water fern Azolla spp. harbors as an endobiont the N2-fixing, filamentous, heterocyst-forming cyanobacterium Nostoc azollae. N. azollae provides the fern with fixed nitrogen permitting its growth in nitrogen-poor environments.
Cristina Sarasa-Buisán +5 more
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New Insights on the Role of ß-Cyanoalanine Synthase CAS-C1 in Root Hair Elongation through Single-Cell Proteomics [PDF]
Plants, 2023 (1) Background: Root hairs are specialized structures involved in water and plant nutrient uptake. They elongate from epidermal cells following a complex developmental program.
Lucía Arenas-Alfonseca +3 more
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Evolution of multicellularity genes in Cyanobacteria in the lead up to the great oxidation event [PDF]
Communications BiologyCyanobacteria, considered responsible for the Great Oxidation Event (GOE) that shaped the Earth for the evolution of complex life, are among the most morphologically diverse prokaryotic phyla.
Joanne S. Boden +5 more
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