Results 31 to 40 of about 2,340 (247)
Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
Discerning protein pools by selective staining with self‐labeling tags
FEBS Letters, EarlyView.Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley +1 more source
FEBS Open Bio, EarlyView.Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley +1 more source
Quantum Reports, 2023 In this work, an alternative attempt to motivate the Many-Worlds Interpretation (MWI) is undertaken. The usual way of arguing for MWI mostly revolves around how it might solve the measurement problem in a more straightforward and concise manner than ...
Michael Huber
doaj +1 more source
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Optimal tests of genuine multipartite nonlocality
New Journal of Physics, 2022 We propose an optimal and efficient numerical test for witnessing genuine multipartite nonlocality based on a geometric approach. In particular, we consider two non-equivalent models of local hidden variables, namely the Svetlichny and the no-signaling ...
Mahasweta Pandit +4 more
doaj +1 more source
FEBS Open Bio, EarlyView.The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong +3 more
wiley +1 more source
Conservation of information and the foundations of quantum mechanics
EPJ Web of Conferences, 2015 We review a recent approach to the foundations of quantum mechanics inspired by quantum information theory [1, 2]. The approach is based on a general framework, which allows one to address a large class of physical theories which share basic information ...
Chiribella Giulio, Maria Scandolo Carlo
doaj +2 more sources
Quantum Superpositions of Causal Structures
Critical Hermeneutics, 2020 This presentation provides a non-technical overview of the notion of quantum superposition of causal structures, of its applications, and of its proposed physical realizations.
Giulio Chiribella
doaj +1 more source