Results 141 to 150 of about 2,668,291 (311)

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Homotopy Perturbation Method Combined with ZZ Transform to Solve Some Nonlinear Fractional Differential Equations

International Journal of Analysis and Applications, 2019
The idea proposed in this work is to extend the ZZ transform method to resolve the nonlinear fractional partial differential equations by combining them with the so-called homotopy perturbation method (HPM).
Lakhdar Riabi, Kacem Belghaba, Mountassir Hamdi Cherif, Djelloul Ziane   +3 more
doaj   +2 more sources

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

The closed-form solution by the exponential rational function method for the nonlinear variable-order fractional differential equations

Frontiers in Physics
The symmetry features of fractional differential equations allow effective explanation of physical and biological phenomena in nature. The generalized form of the fractional differential equations is the variable-order fractional differential equations ...
Mashael M. ALBaidani, Umair Ali, Abdul Hamid Ganie   +2 more
doaj   +1 more source

Applications of the Fractional Calculus: On a Discretization of Fractional Diffusion Equation in One Dimension

, 2010
The paper discusses the problem of classical and fractional diffusion models. It is known that the classical model fails in heterogeneous structures with locations where particles move at a large speed over a long distance.
Tomas Kisela
core   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Continuous extensions of numerical methods for Stochastic Fractional Differential Equations

, 2021
We present a technique to provide continuous-time extension of numerical methods solving Stochastic Fractional Differential Equations (SFDEs). The basic idea we follow is closely related to the classic scenario of deterministic collocation methods for ...
Giordano Giuseppe, Paternoster Beatrice, D'Ambrosio Raffaele, Conte Dajana   +3 more
core  

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

Fractional Time-Delayed Differential Equations: Applications in Cosmological Studies

Fractal and Fractional
Fractional differential equations model processes with memory effects, providing a realistic perspective on complex systems. We examine time-delayed differential equations, discussing first-order and fractional Caputo time-delayed differential equations.
Bayron Micolta-Riascos, Byron Droguett, Gisel Mattar Marriaga, Genly Leon, Andronikos Paliathanasis, Luis del Campo, Yoelsy Leyva   +6 more
doaj   +1 more source