Results 51 to 60 of about 124,436 (296)

Extracellular vesicle and particle isolation from human and murine cell lines, tissues, and bodily fluids

STAR Protocols, 2021
Summary: We developed a modified protocol, based on differential ultracentrifugation (dUC), to isolate extracellular vesicles and particles (specifically exomeres) (EVPs) from various human and murine sources, including cell lines, surgically resected ...
Linda Bojmar, Han Sang Kim, Gabriel C. Tobias, Fanny A. Pelissier Vatter, Serena Lucotti, Kofi Ennu Gyan, Candia M. Kenific, Zurong Wan, Kyung-A Kim, DooA Kim, Jonathan Hernandez, Virginia Pascual, Todd E. Heaton, Michael P. La Quaglia, David Kelsen, Tanya M. Trippett, David R. Jones, William R. Jarnagin, Irina R. Matei, Haiying Zhang, Ayuko Hoshino, David Lyden   +21 more
doaj   +1 more source

Development of an advanced on-line position-specific stable carbon isotope system and application to methyl tert-butyl ether [PDF]

, 2009
We present an advanced system for on-line position-specific carbon isotope analysis. The main limitation of on-line intramolecular isotope ratio measurements has been that optimal pyrolytic fragments are obtained mostly at temperatures where the analyte ...
Abelson, Barrie, Barth, Benson, Coplen, Corso, Corso, Corso, Daly, Dempster, Dias, Elsner, Elsner, Fischer, Gray, Hall, Hall, Harrington, Hayes, Hirschorn, Huang, Hunkeler, Jasper, Jeffrey, Kolhatkar, Kuder, Mancini, Mariotti, Matthews, Meckenstock, Meier-Augenstein, Meier-Augenstein, Merritt, Miller, Monson, Morrison, O'Sullivan, Poulson, Richnow, Rosell, Sacks, Sano, Schmidt, Schmidt, Schüth, Slater, Slater, Slater, Smallwood, Somsamak, Somsamak, Vogler, Wang, Wolyniak, Wolyniak, Yamada, Zwank   +56 more
core   +1 more source

Plecstatin inhibits hepatocellular carcinoma tumorigenesis and invasion through cytolinker plectin

Molecular Oncology, EarlyView.
The ruthenium‐based metallodrug plecstatin exerts its anticancer effect in hepatocellular carcinoma (HCC) primarily through selective targeting of plectin. By disrupting plectin‐mediated cytoskeletal organization, plecstatin inhibits anchorage‐dependent growth, cell polarization, and tumor cell dissemination.
Zuzana Outla, Jan Kosla, Magdalena Prechova, Lukas Frick, Patricia Bortel, Yasmin Borutzki, Andrea Bileck, Christopher Gerner, Samuel M. Meier‐Menches, Selma Osmanagic‐Myers, Martin Gregor   +10 more
wiley   +1 more source

Isospin fractionation in the nucleon emissions and fragment emissions in the intermediate energy heavy ion collisions

, 2003
The degree of isospin fractionation is measured by $(N/Z)_{n}$ / $(N/Z)_{N_{imf}}$, where $(N/Z)_{n}$ and $(N/Z)_{N_{imf}}$ are the saturated neutron-proton ratio of nucleon emissions (gas phase) and that of fragment emissions (liquid phase) in heavy ion
Aichelin, Aichelin, Alkahzov, Baran, Bertsch, Colonna, Danielewicz, Di Toro, Hang Liu, Jian-Ye Liu, Klakow, Kunde, Li, Li, Li, Li, Li, Liu, Liu, Liu, Miller, Pak, Pak, Pak, Pak, Rami, Reinhard, Sobotka, Sobotka, Tsang, Udo Schröder, Wada, Wen-Jun Guo, Westfall, Westfall, Xu, Yang, Yennello, Yennello, Yong Zhong Xing   +39 more
core   +1 more source

Deciphering transcriptional plasticity in pancreatic ductal adenocarcinoma reveals alterations in sensory neuron innervation

Molecular Oncology, EarlyView.
Pancreatic sensory neurons innervating healthy and PDAC tissue were retrogradely labeled and profiled by single‐cell RNA sequencing. Tumor‐associated innervation showed a dominant neurofilament‐positive subtype, altered mitochondrial gene signatures, and reduced non‐peptidergic neurons.
Elena Genova, Michele Montrone, Uday Rangaswamy, Francesco Diversi, Irene Schiavo, Denise Ferrarini, Roberta Di Florio, Irene Longo, Michele Coscia, Nicola Zamboni, Giorgia Demontis, Lisa Veghini, Vincenzo Corbo, Remo Sanges, Paul Heppenstall   +14 more
wiley   +1 more source

Preparation of tailor-made supported catalysts using asymmetric flow field flow fractionation and their application in hydrogenation

Nanotechnology Reviews, 2014
The optical, chemical, and catalytic properties of metallic nanoparticles (NPs) depend strongly on their particle size and shape. Therefore, the preparation of monodisperse metallic NPs is very important for fundamental studies and practical applications.
Baumgard Jens, Pohl Marga-Martina, Kragl Udo, Steinfeldt Norbert   +3 more
doaj   +1 more source

Accelerated Particle Separation in a DLD Device at Re > 1 Investigated by Means of µPIV

Micromachines, 2019
A pressure resistant and optically accessible deterministic lateral displacement (DLD) device was designed and microfabricated from silicon and glass for high-throughput fractionation of particles between 3.0 and 7.0 µm comprising array segments of ...
Jonathan Kottmeier, Maike Wullenweber, Sebastian Blahout, Jeanette Hussong, Ingo Kampen, Arno Kwade, Andreas Dietzel   +6 more
doaj   +1 more source

Application of Af4-Multidetection to Liraglutide in Its Formulation: Preserving and Representing Native Aggregation

Molecules, 2022
Aggregation is among the most critical parameters affecting the pharmacological and safety profile of peptide Active Pharmaceutical Ingredients (APIs).
Valentina Marassi, Marco Macis, Stefano Giordani, Lucia Ferrazzano, Alessandra Tolomelli, Barbara Roda, Andrea Zattoni, Antonio Ricci, Pierluigi Reschiglian, Walter Cabri   +9 more
doaj   +1 more source

E2A selectively regulates TGF‐β–induced apoptosis in KRAS‐mutant non‐small cell lung cancer

Molecular Oncology, EarlyView.
Ability to induce apoptosis by TGF‐β is frequently lost in advanced lung adenocarcinoma despite intact TGF‐β signaling. We identify E2A as a mutant KRAS–dependent mediator of resistance to TGF‐β–induced apoptosis. TGF‐β induces E2A via SMAD3 in mutant KRAS cells, and E2A silencing restores apoptosis and enhances radiation response in cell lines ...
Sergei Chuikov, Shiva Krishna Katkam, Zhefan Wang, Venkateshwar G. Keshamouni   +3 more
wiley   +1 more source

Aptamer–Target–Gold Nanoparticle Conjugates for the Quantification of Fumonisin B1

Biosensors, 2021
Fumonisin B1 (FB1), a mycotoxin classified as group 2B hazard, is of high importance due to its abundance and occurrence in varied crops. Conventional methods for detection are sensitive and selective; however, they also convey disadvantages such as long
Vicente Antonio Mirón-Mérida, Yadira González-Espinosa, Mar Collado-González, Yun Yun Gong, Yuan Guo, Francisco M. Goycoolea   +5 more
doaj   +1 more source