Results 81 to 90 of about 887,812 (350)

Peroxidasin enables melanoma immune escape by inhibiting natural killer cell cytotoxicity

Molecular Oncology, EarlyView.
Peroxidasin (PXDN) is secreted by melanoma cells and binds the NK cell receptor NKG2D, thereby suppressing NK cell activation and cytotoxicity. PXDN depletion restores NKG2D signaling and enables effective NK cell–mediated melanoma killing. These findings identify PXDN as a previously unrecognized immune evasion factor and a potential target to improve
Hsu‐Min Sung, David Bickel, Lena C. M. Krause, Daria Ezeriņa, Christian Ickes, Julian Wojtachnia, Christine S. Gibhardt, Magdalena Shumanska, Khadija Wahni, Andrea Paluschkiwitz, Julia Malo Pueyo, Ekaterina Baranova, Wim Vranken, Hedwig Stanisz, Ioana Stejerean‐Todoran, Michael P. Schön, Joris Messens, Ivan Bogeski   +17 more
wiley   +1 more source

Gel filtration chromatography [PDF]

, 2011
Gel-filtration chromatography is a popular and versatile technique that permits the effective separation of proteins and other biological molecules in high yield. Here, the basis of the method is described and typical matrix types are contrasted.
Cummins, Phil, O'Connor, Brendan, Ó Fágáin, Ciarán   +2 more
core  

Efficiency of size-dependent particle separation by pinched flow fractionation

, 2012
Pinched flow fractionation is shown to be an efficient and selective way to quickly separate particles by size in a very polydisperse semi-concentrated suspension. In an effort to optimize the method, we discuss the quantitative influence of the pinching
Coupier, Gwennou, Podgorski, Thomas, Srivastav, Aparna   +2 more
core   +4 more sources

Soil methane sink capacity response to a long-term wildfire chronosequence in Northern Sweden [PDF]

, 2015
Boreal forests occupy nearly one fifth of the terrestrial land surface and are recognised as globally important regulators of carbon (C) cycling and greenhouse gas emissions.
A Liptay, A Priemé, AJ Soja, Andy Stott, APS Adamsen, ASK Chan, C Knief, CJA Bradshaw, DA Wardle, DA Wardle, DA Wardle, DA Wardle, David A. Wardle, DS Reay, E Chuvieco, EA Holland, ES Kasischke, ES Kasischke, G Girardin, J. Colin Murrell, K Jaatinen, KA Smith, M Ohlson, MA Gathany, Md. Tanvir Rahman, N Pluchon, Niall P. McNamara, Nick J. Ostle, NP McNamara, PJ Maxfield, PSA Beck, R. Michael Lehman, RA Burke, Richard D. Bardgett, Ruth Gregg, S Sjögersten, SC Tyler, Simon Oakley, T Nakano, WS Reeburgh, Y-H Zhang, YA Teh, YA Trotsenko   +42 more
core   +3 more sources

Rethinking plastic waste: innovations in enzymatic breakdown of oil‐based polyesters and bioplastics

FEBS Open Bio, EarlyView.
Plastic pollution remains a critical environmental challenge, and current mechanical and chemical recycling methods are insufficient to achieve a fully circular economy. This review highlights recent breakthroughs in the enzymatic depolymerization of both oil‐derived polyesters and bioplastics, including high‐throughput protein engineering, de novo ...
Elena Rosini, Nicolò Antonelli, Gianluca Molla   +2 more
wiley   +1 more source

Characterization of seed nuclei in glucagon aggregation using light scattering methods and field-flow fractionation

Journal of Biological Engineering, 2008
Background Glucagon is a peptide hormone with many uses as a therapeutic agent, including the emergency treatment of hypoglycemia. Physical instability of glucagon in solution leads to problems with the manufacture, formulation, and delivery of this ...
Kirsch Lee E, Nguyen Lida T, Hoppe Cindy C, Wiencek John M   +3 more
doaj   +1 more source

Asymmetric flow field‐flow fractionation of liposomes: optimization of fractionation variables [PDF]

Journal of Separation Science, 2009
AbstractThe purpose of this study was to investigate the influence of ionic strength of the carrier liquid, cross flow rate, focus flow rate, and sample load on the retention behavior of liposomes in asymmetric flow field‐flow fractionation (AF4). Two differently prepared samples of large unilamellar vesicles (LUV) were used. Experiments were performed
Stefan, Hupfeld, Dominik, Ausbacher, Martin, Brandl   +2 more
openaire   +3 more sources

Intercomparison of soil pore water extraction methods for stable isotope analysis [PDF]

, 2016
Funded by NSERC Discovery Grant U.S. Forest Service U.S. Department of Energy's Office of Energy Efficiency and Renewable Energy, Bioenergy Technologies OfficePeer ...
McDonnell, Jeffery J., Orlowski, Natalie, Pratt, Dylan L.   +2 more
core   +1 more source

Hands‐on protocol for preparing water‐soluble fractions from agri‐food samples for NMR‐based metabolomics analysis

FEBS Open Bio, EarlyView.
This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso, Jaime Hiniesta‐Valero, Alejandra Guerra‐Castellano, Miguel A. De la Rosa, Irene Díaz‐Moreno   +4 more
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source