Identification of (20R)‐protopanaxadiol from Panax ginseng as a novel anti‐SARS‐CoV‐2 compound
FEBS Open Bio, EarlyView.We established a noninfectious BAC‐based SARS‐CoV‐2 replicon that enables antiviral screening under BSL‐2 conditions. Using this platform, we screened 373 food‐derived compounds and identified (20R)‐protopanaxadiol from Panax ginseng as a novel inhibitor of SARS‐CoV‐2 RNA replication, highlighting the value of safe replicon systems for antiviral ...
Midori Takeda +4 more
wiley +1 more source
Fragmentation of Agricultural Land Parcels [PDF]
This study seeks to take fragmentation research in a new direction by looking at exurban sprawl and fragmentation of ownership. The primary objective of this study is to identify the location and magnitude of fragmentation of agricultural land parcels ...
Neal, Kalyn +2 more
core
FEBS Open Bio, EarlyView.Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
What does the evidence tell us about fragmentation and outsourcing [PDF]
The paper studies fragmentation and outsourcing at the international level.Fragmentation ...
Ronald Jones; Henryk Kierzkowski; Chen Lurong
core
Fragment Coverage: Unified Visualization and Quantitative Assessment of MS/MS Fragmentation Supports Metabolite Identification in Macromolecule Drug Metabolism Studies. [PDF]
Rapid Commun Mass SpectromCifuentes P +3 more
europepmc +1 more source
Adenosine triphosphate as a modulator of protein interactions and stability
FEBS Open Bio, EarlyView.ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley +1 more source
Implementation of Nano Flow Chromatography Coupled to Mass Spectrometry as a Reliable and Sensitive Discovery Lipidomics Platform. [PDF]
Rapid Commun Mass SpectromSallowicz CJ +4 more
europepmc +1 more source
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
The Impact of Production Fragmentation on Industry Skill Upgrading: New Evidence from Japanese Manufacturing [PDF]
This paper examines the hypothesis that industries engaged in international fragmentation of production experience greater skill upgrading using a panel dataset of Japanese manufacturing over the period 1980-2000.
Nobuaki Yamashita
core
Revisiting the Fragmentation of Ketoconazole by Positive Electrospray Ionization Tandem Mass Spectrometry and Density Functional Theory Calculations. [PDF]
Rapid Commun Mass SpectromCarnevale Neto F, Vessecchi R.
europepmc +1 more source